LOCUS       CDS_LUC                 3711 bp ds-DNA     linear       29-OCT-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-B09" "Type.plasmid:L0"          
            "Description" "Use:Firefly luciferase" "Plasmid size, bp:3711"
            "Bacteria resistance:Cm" "Plant resistance" "Phytozome.ID.v3"
            "Reference:Sauret-Gueto S, Frangedakis E, et al (in preparation)"
            "Primary publication:New Phytologist(2017)216\:576–590doi\:
            10.1111/nph.14487" "Registry.ID.JHLab:HMO_p0_o043" "Name.JHLab"
FEATURES             Location/Qualifiers
     misc_feature    247..1896
                     /label="firefly luciferase"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(1902..1907)
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Terminator      complement(2937..3031)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1897..1900
                     /label="L0 fusion site GCTT"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(3052..3711)
                     /label="cat / CmR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    236..241
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    243..246
                     /label="L0 fusion site AATG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     Replication_ori complement(2166..2754)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1051..1057
                     /label="Restriction site removed"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct
      241 cAaatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat
      301 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
      361 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg
      421 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc
      481 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga
      541 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc
      601 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg
      661 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag
      721 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac
      781 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct
      841 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc
      901 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt
      961 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga
     1021 tttcgagtcg tcttaatgta tagatttgaa gaggagctgt ttctgaggag ccttcaggat
     1081 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact
     1141 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc
     1201 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga
     1261 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg
     1321 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg
     1381 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc
     1441 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat
     1501 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag
     1561 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc
     1621 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa
     1681 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg
     1741 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg
     1801 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc
     1861 ataaaggcca agaagggcgg aaagatcgcc gtgtaagctt CgagaccCTG CAGTCCGGCA
     1921 AAAAAGGGCA AGGTGTCACC ACCCTGCCCT TTTTCTTTAA AACCGAAAAG ATTACTTCGC
     1981 GTTATGCAGG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG
     2041 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA
     2101 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
     2161 GCGTTTTTCC ACAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG
     2221 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
     2281 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG
     2341 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT
     2401 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
     2461 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC
     2521 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
     2581 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA
     2641 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC
     2701 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT
     2761 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     2821 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     2881 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGCTCGAG GCTTGGATTC
     2941 TCACCAATAA AAAACGCCCG GCGGCAACCG AGCGTTCTGA ACAAATCCAG ATGGAGTTCT
     3001 GAGGTCATTA CTGGATCTAT CAACAGGAGT CCAAGCGAGC TCGATATCAA ATTACGCCCC
     3061 GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC
     3121 ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT
     3181 ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA
     3241 ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA
     3301 CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG
     3361 TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG
     3421 CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT
     3481 CATTGCCATA CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC
     3541 CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG
     3601 AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG
     3661 ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA T
//