LOCUS       CDS_GUS                 3870 bp ds-DNA     circular     04-NOV-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-B10" "Type.plasmid:L0"          
            "Description" "Use:For histochemical β-glucuronidase activity
            assays. This GUS CDS does not have an intron." "Plasmid size,
            bp:3867" "Bacteria resistance:Cm" "Plant resistance"
            "Phytozome.ID.v3" "Reference:Sauret-Gueto S, Frangedakis E, et al
            (in preparation)" "Primary
            publication:https\://doi.org/10.1002/j.1460-2075.1987.tb02730.x
            https\://doi.org/10.1093/pcp/pcn085"
            "Registry.ID.JHLab:HMO_p0_o971" "Name.JHLab"
FEATURES             Location/Qualifiers
     Terminator      complement(3096..3190)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1146..1146
                     /label="A>G"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    236..241
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Replication_ori complement(2325..2913)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2056..2059
                     /label="L0 fusion site GCTT"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(3211..3870)
                     /label="cat / CmR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    244..2055
                     /label="uidA / GUS"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1078..1078
                     /label="C>G , seq AO was not ok"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1501..1501
                     /label="insertion-diff with AO lab"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1518..1518
                     /label="insertion -diff with seq from AO lab"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    243..246
                     /label="L0 fusion site AATG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2061..2066)
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct
      241 cGAatgttac gtcctgtaga aaccccaacc cgtgaaatca aaaaactcga cggcctgtgg
      301 gcattcagtc tggatcgcga aaactgtgga attgatcagc gttggtggga aagcgcgtta
      361 caagaaagcc gggcaattgc tgtgccaggc agttttaacg atcagttcgc cgatgcagat
      421 attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag tctttatacc gaaaggttgg
      481 gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc attacggcaa agtgtgggtc
      541 aataatcagg aagtgatgga gcatcagggc ggctatacgc catttgaagc cgatgtcacg
      601 ccgtatgtta ttgccgggaa aagtgtacgt atcaccgttt gtgtgaacaa cgaactgaac
      661 tggcagacta tcccgccggg aatggtgatt accgacgaaa acggcaagaa aaagcagtct
      721 tacttccatg atttctttaa ctatgccggg atccatcgca gcgtaatgct ctacaccacg
      781 ccgaacacct gggtggacga tatcaccgtg gtgacgcatg tcgcgcaaga ctgtaaccac
      841 gcgtctgttg actggcaggt ggtggccaat ggtgatgtca gcgttgaact gcgtgatgcg
      901 gatcaacagg tggttgcaac tggacaaggc actagcggga ctttgcaagt ggtgaatccg
      961 cacctctggc aaccgggtga aggttatctc tatgaactgt gcgtcacagc caaaagccag
     1021 acagagtgtg atatctaccc gcttcgcgtc ggcatccggt cagtggcagt gaagggcGaa
     1081 cagttcctga ttaaccacaa accgttctac tttactggct ttggtcgtca tgaagatgcg
     1141 gacttGcgtg gcaaaggatt cgataacgtg ctgatggtgc acgaccacgc attaatggac
     1201 tggattgggg ccaactccta ccgtacctcg cattaccctt acgctgaaga gatgctcgac
     1261 tgggcagatg aacatggcat cgtggtgatt gatgaaactg ctgctgtcgg ctttaacctc
     1321 tctttaggca ttggtttcga agcgggcaac aagccgaaag aactgtacag cgaagaggca
     1381 gtcaacgggg aaactcagca agcgcactta caggcgatta aagagctgat agcgcgtgac
     1441 aaaaaccacc caagcgtggt gatgtggagt attgccaacg aaccggatac ccgtccgcaa
     1501 Ggtgcacggg aatatttCGc gccactggcg gaagcaacgc gtaaactcga cccgacgcgt
     1561 ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca ccgataccat cagcgatctc
     1621 tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg tccaaagcgg cgatttggaa
     1681 acggcagaga aggtactgga aaaagaactt ctggcctggc aggagaaact gcatcagccg
     1741 attatcatca ccgaatacgg cgtggatacg ttagccgggc tgcactcaat gtacaccgac
     1801 atgtggagtg aagagtatca gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc
     1861 gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg attttgcgac ctcgcaaggc
     1921 atattgcgcg ttggcggtaa caagaaaggg atcttcactc gcgaccgcaa accgaagtcg
     1981 gcggcttttc tgctgcaaaa acgctggact ggcatgaact tcggtgaaaa accgcagcag
     2041 ggaggcaaac aatgaGCTTT gagaccCTGC AGTCCGGCAA AAAAGGGCAA GGTGTCACCA
     2101 CCCTGCCCTT TTTCTTTAAA ACCGAAAAGA TTACTTCGCG TTATGCAGGC TTCCTCGCTC
     2161 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
     2221 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC
     2281 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA CAGGCTCCGC
     2341 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
     2401 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
     2461 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
     2521 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG
     2581 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
     2641 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
     2701 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT
     2761 AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
     2821 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG
     2881 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG
     2941 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     3001 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     3061 TATGAGTAAA CTTGGTCTGA CAGCTCGAGG CTTGGATTCT CACCAATAAA AAACGCCCGG
     3121 CGGCAACCGA GCGTTCTGAA CAAATCCAGA TGGAGTTCTG AGGTCATTAC TGGATCTATC
     3181 AACAGGAGTC CAAGCGAGCT CGATATCAAA TTACGCCCCG CCCTGCCACT CATCGCAGTA
     3241 CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC
     3301 CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA
     3361 AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC
     3421 CCAGGGATTG GCTGAGACGA AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG
     3481 GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC
     3541 GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA
     3601 AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GAAATTCCGG
     3661 ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT
     3721 TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA
     3781 TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC
     3841 GGTGGTATAT CCAGTGATTT TTTTCTCCAT
//