LOCUS       CDS12_mScarlet-I        2756 bp ds-DNA     linear       04-NOV-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-C01" "Type.plasmid:L0"          
            "Description:CDS12_mScarletI(noStop)" "Use:FP for N-term fusion
            with a CTAG" "Plasmid size, bp:2756" "Bacteria resistance:Cm"
            "Plant resistance" "Phytozome.ID.v3" "Reference:Sauret-Gueto S,
            Frangedakis E, et al (in preparation)" "Primary
            publication:https\://doi.org/10.1038/nmeth.4074"
            "Registry.ID.JHLab:HMO_p0_o948" "Name.JHLab"
FEATURES             Location/Qualifiers
     primer_bind     645..664
                     /label="mCherry-F"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Replication_ori complement(1211..1799)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    243..246
                     /label="L0 fusion site AATG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     Overhang        942..945
                     /label="CDS- overhang"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(2097..2756)
                     /label="cat / CmR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    236..241
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             244..939
                     /label="mScarlet-I no Stop"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(377..395)
                     /label="mCherry-R"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Terminator      complement(1982..2076)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(947..952)
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCGGTCT
      241 CaAatggtga gcaagggcga ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg
      301 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      361 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttctcctgg
      421 gacatcctgt cccctcagtt catgtacggc tccagggcct tcatcaagca ccccgccgac
      481 atccccgact actataagca gtccttcccc gagggcttca agtgggagcg cgtgatgaac
      541 ttcgaggacg gcggcgccgt gaccgtgacc caggacacct ccctggagga cggcaccctg
      601 atctacaagg tgaagctccg cggcaccaac ttccctcctg acggccccgt aatgcagaag
      661 aagacaatgg gctgggaagc gtccaccgag cggttgtacc ccgaggacgg cgtgctgaag
      721 ggcgacatta agatggccct gcgcctgaag gacggcggcc gctacctggc ggacttcaag
      781 accacctaca aggccaagaa gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag
      841 ttggacatca cctcccacaa cgaggactac accgtggtgg aacagtacga acgctccgag
      901 ggccgccact ccaccggcgg catggacgag ctgtacaagg cttcgtGAGA CCCTGCAGTC
      961 CGGCAAAAAA GGGCAAGGTG TCACCACCCT GCCCTTTTTC TTTAAAACCG AAAAGATTAC
     1021 TTCGCGTTAT GCAGGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC
     1081 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG
     1141 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT
     1201 TGCTGGCGTT TTTCCACAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA
     1261 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT
     1321 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC
     1381 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG
     1441 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT
     1501 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG
     1561 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA
     1621 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA
     1681 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG
     1741 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG
     1801 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG
     1861 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT
     1921 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGC TCGAGGCTTG
     1981 GATTCTCACC AATAAAAAAC GCCCGGCGGC AACCGAGCGT TCTGAACAAA TCCAGATGGA
     2041 GTTCTGAGGT CATTACTGGA TCTATCAACA GGAGTCCAAG CGAGCTCGAT ATCAAATTAC
     2101 GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG
     2161 AAGCCATCAC AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT
     2221 TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG
     2281 TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA
     2341 ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT
     2401 ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA
     2461 GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG
     2521 TCTTTCATTG CCATACGAAA TTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA
     2581 AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC
     2641 AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT
     2701 TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCAT
//