LOCUS       CDS12_mScarletI-linker  2789 bp ds-DNA     circular     04-NOV-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-C02" "Type.plasmid:L0"          
            "Description:CDS12_mScarletI(noStop)-SerGly10xAlaSer" "Use:FP for
            N-term fusion with a CTAG without linker" "Plasmid size, bp:2789"
            "Bacteria resistance:Cm" "Plant resistance" "Phytozome.ID.v3"
            "Reference:Sauret-Gueto S, Frangedakis E, et al (in preparation)"
            "Primary publication:https\://doi.org/10.1038/nmeth.4074"
            "Registry.ID.JHLab:HMO_p0_o990" "Name.JHLab"
FEATURES             Location/Qualifiers
     misc_feature    236..241
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(377..395)
                     /label="mCherry-R"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     645..664
                     /label="mCherry-F"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    940..978
                     /label="linker SG10xAlaS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(2130..2789)
                     /label="cat / CmR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     Replication_ori complement(1244..1832)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             244..939
                     /label="mScarlet-I no Stop"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(980..985)
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Terminator      complement(2015..2109)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    975..978
                     /label="L0 fusion site TTCG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct
      241 cGAatggtga gcaagggcga ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg
      301 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      361 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttctcctgg
      421 gacatcctgt cccctcagtt catgtacggc tccagggcct tcatcaagca ccccgccgac
      481 atccccgact actataagca gtccttcccc gagggcttca agtgggagcg cgtgatgaac
      541 ttcgaggacg gcggcgccgt gaccgtgacc caggacacct ccctggagga cggcaccctg
      601 atctacaagg tgaagctccg cggcaccaac ttccctcctg acggccccgt aatgcagaag
      661 aagacaatgg gctgggaagc gtccaccgag cggttgtacc ccgaggacgg cgtgctgaag
      721 ggcgacatta agatggccct gcgcctgaag gacggcggcc gctacctggc ggacttcaag
      781 accacctaca aggccaagaa gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag
      841 ttggacatca cctcccacaa cgaggactac accgtggtgg aacagtacga acgctccgag
      901 ggccgccact ccaccggcgg catggacgag ctgtacaagt ccggagctgc ggccgctgcc
      961 gctgcggcag cggcttcgTg agAccCTGCA GTCCGGCAAA AAAGGGCAAG GTGTCACCAC
     1021 CCTGCCCTTT TTCTTTAAAA CCGAAAAGAT TACTTCGCGT TATGCAGGCT TCCTCGCTCA
     1081 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG
     1141 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC
     1201 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAC AGGCTCCGCC
     1261 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
     1321 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
     1381 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA
     1441 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
     1501 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA
     1561 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG
     1621 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA
     1681 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG
     1741 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC
     1801 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT
     1861 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA
     1921 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT
     1981 ATGAGTAAAC TTGGTCTGAC AGCTCGAGGC TTGGATTCTC ACCAATAAAA AACGCCCGGC
     2041 GGCAACCGAG CGTTCTGAAC AAATCCAGAT GGAGTTCTGA GGTCATTACT GGATCTATCA
     2101 ACAGGAGTCC AAGCGAGCTC GATATCAAAT TACGCCCCGC CCTGCCACTC ATCGCAGTAC
     2161 TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAAACGGC ATGATGAACC
     2221 TGAATCGCCA GCGGCATCAG CACCTTGTCG CCTTGCGTAT AATATTTGCC CATGGTGAAA
     2281 ACGGGGGCGA AGAAGTTGTC CATATTGGCC ACGTTTAAAT CAAAACTGGT GAAACTCACC
     2341 CAGGGATTGG CTGAGACGAA AAACATATTC TCAATAAACC CTTTAGGGAA ATAGGCCAGG
     2401 TTTTCACCGT AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG GAAATCGTCG
     2461 TGGTATTCAC TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC GGTGTAACAA
     2521 GGGTGAACAC TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG AAATTCCGGA
     2581 TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT
     2641 TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT
     2701 TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG
     2761 GTGGTATATC CAGTGATTTT TTTCTCCAT
//