LOCUS       CDS12_TP-MpSIG2         2360 bp ds-DNA     circular     04-NOV-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-C07" "Type.plasmid:L0"          
            "Description" "Use:Chloroplast transit peptide from MpSIG2. For
            N-term fusion with a CTAG" "Plasmid size, bp:2360" "Bacteria
            resistance:Cm" "Plant resistance" "Phytozome.ID.v3:SIG2
            Mapoly0214s0004" "Reference:Sauret-Gueto S, Frangedakis E, et al
            (in preparation)" "Primary publication:Plant Cell Physiol. 54(10)\:
            1736–1748 (2013) doi\:10.1093/pcp/pct119,"
            "Registry.ID.JHLab:HMO_p0_o036" "Name.JHLab"
FEATURES             Location/Qualifiers
     misc_feature    546..549
                     /label="L0 fusion site TTCG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    236..241
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    244..543
                     /label="TP-MpSIG2"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     Replication_ori complement(815..1403)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             complement(1701..2360)
                     /label="cat / CmR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    243..246
                     /label="L0 fusion site "
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     Terminator      complement(1586..1680)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(551..556)
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    339..345
                     /label="Restriction site removed"
                     /ApEinfo_revcolor=#f96381
                     /ApEinfo_fwdcolor=#f96381
     misc_feature    323..329
                     /label="Restriction site removed"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCGGTCT
      241 CAaatggcgg ttgttgctac gagcgagctg ctgcgcttgc acgactcgat cacctgctcg
      301 ccgcatgctt cgtcccgtga tggctcgtcc ggtgttgtga aaagcttgag gcgaagttct
      361 gggaccagca ggaagcgagt tactctcaag caacagagag cttttggacc taattcttct
      421 cggtttagcg tagaaactac ttgtgcacct tcgagcattt cttacagccc ctgtcgaagg
      481 ccatccaggt tgtcggcctt tgatcgaatt caaaatttgc ctgtcactgc tgcatctgcc
      541 ggggcttcgC GAGACCCTGC AGTCCGGCAA AAAAGGGCAA GGTGTCACCA CCCTGCCCTT
      601 TTTCTTTAAA ACCGAAAAGA TTACTTCGCG TTATGCAGGC TTCCTCGCTC ACTGACTCGC
      661 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT
      721 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG
      781 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA CAGGCTCCGC CCCCCTGACG
      841 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT
      901 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
      961 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT
     1021 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC
     1081 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA
     1141 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG
     1201 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG
     1261 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT
     1321 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA
     1381 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC
     1441 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA
     1501 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA
     1561 CTTGGTCTGA CAGCTCGAGG CTTGGATTCT CACCAATAAA AAACGCCCGG CGGCAACCGA
     1621 GCGTTCTGAA CAAATCCAGA TGGAGTTCTG AGGTCATTAC TGGATCTATC AACAGGAGTC
     1681 CAAGCGAGCT CGATATCAAA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT
     1741 TCATTAAGCA TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC
     1801 AGCGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG
     1861 AAGAAGTTGT CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG
     1921 GCTGAGACGA AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG
     1981 TAACACGCCA CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA
     2041 CTCCAGAGCG ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA
     2101 CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GAAATTCCGG ATGAGCATTC
     2161 ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG
     2221 GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT
     2281 GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT
     2341 CCAGTGATTT TTTTCTCCAT
//