LOCUS       CTAG_linker-Lti6b       2280 bp ds-DNA     circular     04-NOV-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-D01" "Type.plasmid:L0"          
            "Description:CTAG_AlaSerGly10xAla_Lti6b" "Use:Plasma Membrane
            targeting protein for C-term fusion with CDS12 without linker."
            "Plasmid size, bp:2280" "Bacteria resistance:Cm" "Plant resistance"
            "Phytozome.ID.v3" "Reference:Sauret-Gueto S, Frangedakis E, et al
            (in preparation)" "Primary
            publication:https\://doi.org/10.1073/pnas.97.7.3718
            https\://doi.org/10.1371/journal.pone.0138876"
            "Registry.ID.JHLab:HMO_p0_o978" "Name.JHLab"
FEATURES             Location/Qualifiers
     misc_feature    321..485
                     /label="AtRCI2B AT3G05890"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             303..485
                     /label="Lti6b"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Overhang        489..492
                     /label="L0 fusion site GCTT"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Replication_ori 1692..2280
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             735..1394
                     /label="cat / CmR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     Overhang        266..269
                     /label="L0 fusion site TTCG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Terminator      1415..1509
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(494..499)
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    259..264
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    267..302
                     /label="linker SG10xAla"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(500..500)
                     /label="here, keep"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG
       61 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT
      121 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCCTGC
      181 ATAACGCGAA GTAATCTTTT CGGTTTTAAA GAAAAAGGGC AGGGTGGTGA CACCTTGCCC
      241 TTTTTTGCCG GACTGCAGgg tctcGttcgg gagctgcggc agctgccgct gcggcagcgg
      301 ccgaattcaa gcgcttgaaa atgagtacag ccactttcgt agagattatt cttgctatca
      361 tcttgcctcc tctcggcgtc tttctcaaat ttggttgcaa ggttgagttt tggatatgtt
      421 tgattttgac gctgtttggt tatcttcccg gaatccttta cgctctttat atcatcacct
      481 tttgatgagc ttTgagaccG AATTCCAGAA ATCATCCTTA GCGAAAGCTA AGGATTTTTT
      541 TTATCTGAAA TTCTGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT
      601 GGTTTCTTAG ACGTCAGGTG GCACTCGAGT TGATCGGGCA CGTAAGAGGT TCCAACTTTC
      661 ACCATAATGA AATAAGATCA CTACCGGGCG TATTTTTTGA GTTATCGAGA TTTTCAGGAG
      721 CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT
      781 GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA
      841 CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT
      901 ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTTCGTATGG
      961 CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC
     1021 ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT
     1081 TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA
     1141 AAGGGTTTAT TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT
     1201 TTGATTTAAA CGTGGCCAAT ATGGACAACT TCTTCGCCCC CGTTTTCACC ATGGGCAAAT
     1261 ATTATACGCA AGGCGACAAG GTGCTGATGC CGCTGGCGAT TCAGGTTCAT CATGCCGTTT
     1321 GTGATGGCTT CCATGTCGGC AGAATGCTTA ATGAATTACA ACAGTACTGC GATGAGTGGC
     1381 AGGGCGGGGC GTAATTTGAT ATCGAGCTCG CTTGGACTCC TGTTGATAGA TCCAGTAATG
     1441 ACCTCAGAAC TCCATCTGGA TTTGTTCAGA ACGCTCGGTT GCCGCCGGGC GTTTTTTATT
     1501 GGTGAGAATC CAAGCCTCGA GCTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT
     1561 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG
     1621 ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC
     1681 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA
     1741 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG
     1801 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA
     1861 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA
     1921 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG
     1981 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG
     2041 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG
     2101 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG
     2161 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC
     2221 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTGTGGAAA
//