LOCUS       Exported                4788 bp ds-DNA     circular SYN 08-NOV-2019
DEFINITION  5UTR for cloning after PROM_MpNOP1.
ACCESSION   .
VERSION     .
KEYWORDS    5UTR_MpNOP1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4788)
  TITLE     Marchantia polymorpha Type IIS DNA Toolkit
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 18, 2020 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     162..180
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /note="color: black; sequence: AATAGGCGTATCACGAGGC"
     terminator      184..227
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
                     /note="color: #ffffff"
     terminator      2991..3048
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
                     /note="color: #ffffff"
     primer_bind     complement(3072..3089)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     rep_origin      complement(3243..3831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(3323..3342)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     terminator      4014..4108
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
                     /note="color: #ffffff"
     CDS             complement(4129..4788)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4703..4722
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
                     /note="color: black; sequence: GCAACTGACTGAAATGCCTC"
ORIGIN
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcggtct
      241 cgtactggct tgaaccttgt gacttggctg cctagctgtc cattttgtcc gactggttct
      301 cctgggcctt gatggtgcac acaatggagt ccgtgcgagt cagtgggcgg gcagacacag
      361 ctagagcccc cgcactgtga gcagccttca agggaaggcc aggggccgag agaaatgcgc
      421 tccaacggcc cggccccgat gggatggcat ggcatgggag gaggacgagc agcagctggt
      481 tcgcctggag gtgaagaaga gtagaggtgg agtttagtga gtccagttca cagaggaggg
      541 cgccggaaaa gactcctgac tcatgactgg gatcagacac atgatggcgg atgacctttc
      601 tgccttcctg cgcatgactc tgagctcaag tctcatcttg tagtcgaggt gagcgaatga
      661 ctgtgtatgc tcgtagttta catgtttgtt gctcggcctt cgttctgcct caagttcagc
      721 agcaagctca gtcctgggct cagcttcccc cgtctcctct ctctctctat ctccctccct
      781 cgtttctatt tcctctctcg tggtcttcgg tcccgttcgg tctgttctgt agggtggatg
      841 ctattatggt ctgttgaagg gcatcatggg atgggttgga ttgggacgag cagctggaat
      901 ggagtgagtc tgaagctctt attcgggctc gcctcccgct cttggagctg caaacgaagc
      961 cgcgcagcgt cgtgcgtcag ctccacaagt ctgggacgga gggattgatg catatttact
     1021 aagtttacag ccccttcggt tggatcctcg tcggcctctg tcatcattcg ttctgttcgt
     1081 tcgtccgagg cggtgctgtg aaagaacggg tcgtcgctgg gttgggttgg gttgactttc
     1141 gggccaattt gcgagccccg aaccggggac ggacctggct gggccttgat tcttcaattc
     1201 ggccttgacc acgtcacgcc ctcccgcttg tccttgctcg ctgcctcgac cccgcccgga
     1261 cagctagaac gagattcctg gagcccgcag gtggagctta tctcctcctc cccgcgcact
     1321 ccgaattttc ctcgcctctg ctgcctcgga ctgtcgctcg tcccgcccgc gcccaggaga
     1381 cgaggtcccg tgccatctct cgtctgtgtc tctctcggtc ctgttgtgct gctctcttgg
     1441 ccactggagg cgagcaagcg agcaagcgag aaaggaagga agtaaggata ggtgcctgac
     1501 ggcggacgga gccgcagctg gagctggggt cgttcttctt ctttgcgtgt agggagacga
     1561 cgaagtgccg gcattgtaga gatcagcggt gtcacactca cgcctgtgcg aatagtatcg
     1621 gaatgaaata ggtttatgct tgagctctgg ggcgaggggg ctttctgcat tgagttgagc
     1681 gtcggcccga gaaggcgggg aggcaggcag tccaccgagg tcggccatag ctttccttcc
     1741 caacctcgta tgtgctcgcc ggaatgaagg aagagaatga tcatgatact gccgagatgt
     1801 tgcaagagcc cgtggtggga tcagagcatg gagtttgatt tttggtaagc agtatgagtt
     1861 atggttcaga gagtggaagg tggaaagggt gagggagtgg cagtgagcac agcagggtcg
     1921 agcaggctat gtagctggaa tggagtgcga gcaagctcaa gagttttctc ctttggattt
     1981 tccggtagat ttgtaatgct cgagagtaga aattccaggc caactccctc atttgatgtc
     2041 ccgaggggag caagggtctt cttctcatgg gaacggcggt caaattctcg gtggattgaa
     2101 acggggccta gctgggaatt tctcatcgaa agaaattttc agcgctgaaa tacgtccgag
     2161 agatcaagta aggttttgat aacagtgcag aaggggatat tgtctggtgg gttgggattg
     2221 ttgctggcgc agtcaaacga gggaaatact cctaagcgtg gttctgctgt ctttgtcata
     2281 tttcaaagtc ttcatctgtt tttcatcacc cagcgagtgt gagaagtctg aatgtgtgag
     2341 cagaaataaa agggaatttc gagccataag aaccagctaa agaacatcaa acgagctgtg
     2401 agcggactcg tctcttagac ttaagattca ctcaaacgtc attgcgtgta acttgttcct
     2461 aggactgctt ttttggtttg ctgccgtcga tatcgtcaca cgttttgtag tcttccattc
     2521 tcgtgattac cccggaatta ttttcgtctt cacctgaacg attttcttac aaagtggacg
     2581 agatgaacgt gcttgttctc tcgagggccc cggatcgacc ttctgctttt tcgtcctcgt
     2641 ttgatctcct gggatccgag agcttggtca ctcgtcgatg ttttcctcgt tagaaaaagt
     2701 gtaatgggtt agcagtcccc tgcatcagac tgtcaatttc catttgtttt aattggaaga
     2761 aggcccggag tttcccgggt tcgtctttac tctgttcgta tagtgtcgcg tgtgcccagt
     2821 tgttgtattt ccagagtgta ccttgcggtg gagttgactt tttcgtgcca catgaatgta
     2881 aaccgtttga gcactattat ctgttaaggc tgctaatcaa cactgacctc tcaattgcgc
     2941 tgtatgtgga tgcagttagg agccttgtcg aataatgtga gaccctgcag tccggcaaaa
     3001 aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt
     3061 atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     3121 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3181 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3241 tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3301 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3361 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3421 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3481 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3541 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3601 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3661 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3721 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3781 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3841 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3901 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3961 aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct tggattctca
     4021 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     4081 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
     4141 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     4201 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     4261 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     4321 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     4381 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     4441 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     4501 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     4561 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     4621 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     4681 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     4741 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccat
//
