LOCUS       CTAG_mTurquoise2        2778 bp ds-DNA     linear       04-NOV-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-D06" "Type.plasmid:L0"          
            "Description:CTAG_mTurquoise2(noATG)" "Use:FP for C-term fusion
            with CDS12" "Plasmid size, bp:2778" "Bacteria resistance:Cm" "Plant
            resistance" "Phytozome.ID.v3" "Reference:Sauret-Gueto S,
            Frangedakis E, et al (in preparation)" "Primary
            publication:https\://pubs.acs.org/doi/10.1021/bi0516273"
            "Registry.ID.JHLab:HMO_p0_o069" "Name.JHLab"
FEATURES             Location/Qualifiers
     misc_feature    complement(969..974)
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Replication_ori complement(1233..1821)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    964..967
                     /label="CTAG overhang"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(2119..2778)
                     /label="cat / CmR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    236..241
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             247..963
                     /label="mTurquoise2 no ATG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Terminator      complement(2004..2098)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    243..246
                     /label="CTAG overhang"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct
      241 cAttcggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      301 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      361 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      421 accctcgtga ccaccctgtc ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg
      481 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      541 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      601 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      661 cacaagctgg agtacaacta ctttagcgac aacgtctata tcaccgccga caagcagaag
      721 aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc
      781 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      841 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
      901 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
      961 taagcttCga gAccCTGCAG TCCGGCAAAA AAGGGCAAGG TGTCACCACC CTGCCCTTTT
     1021 TCTTTAAAAC CGAAAAGATT ACTTCGCGTT ATGCAGGCTT CCTCGCTCAC TGACTCGCTG
     1081 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA
     1141 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC
     1201 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCACA GGCTCCGCCC CCCTGACGAG
     1261 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC
     1321 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC
     1381 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT
     1441 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC
     1501 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA
     1561 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA
     1621 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA
     1681 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA
     1741 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG
     1801 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     1861 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     1921 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     1981 TGGTCTGACA GCTCGAGGCT TGGATTCTCA CCAATAAAAA ACGCCCGGCG GCAACCGAGC
     2041 GTTCTGAACA AATCCAGATG GAGTTCTGAG GTCATTACTG GATCTATCAA CAGGAGTCCA
     2101 AGCGAGCTCG ATATCAAATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC
     2161 ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG
     2221 CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA
     2281 GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC
     2341 TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA
     2401 ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT
     2461 CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT
     2521 ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGA AATTCCGGAT GAGCATTCAT
     2581 CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT
     2641 CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA
     2701 CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC
     2761 AGTGATTTTT TTCTCCAT
//