LOCUS       PROM5_35S               2923 bp ds-DNA     circular     04-NOV-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-E01" "Type.plasmid:L0"          
            "Description" "Use:35S CaMV Strong promoter that drives expression
            everywhere in Mp gemmae but much weaker in the notches" "Plasmid
            size, bp:2923" "Bacteria resistance:Cm" "Plant resistance"
            "Phytozome.ID.v3" "Reference:Sauret-Gueto S, Frangedakis E, et al
            (in preparation)" "Primary
            publication:https\://doi.org/10.1105/tpc.13.3.495  Transgenic
            Research April 2014, Volume 23, Issue 2, pp 235–244
            https\://doi.org/10.1007/s11248-013-9746-z"
            "Registry.ID.JHLab:HMO_p0_o984" "Name.JHLab"
FEATURES             Location/Qualifiers
     promoter        1030..1075
                     /label="min 35S pro"
                     /ApEinfo_revcolor=#5573ff
                     /ApEinfo_fwdcolor=#5573ff
     misc_feature    1109..1112
                     /label="L0 fusion site AATG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    236..241
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    247..1081
                     /label="p5-35S"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(1114..1119)
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1046..1052
                     /label="TATA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Terminator      complement(2149..2243)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Replication_ori complement(1378..1966)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             complement(2264..2923)
                     /label="cat / CmR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    243..246
                     /label="L0 fusion site GGAG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct
      241 cGGGAGAGAT TAGCCTTTTC AATTTCAGAA AGAATGCTAA CCCACAGATG GTTAGAGAGG
      301 CTTACGCAGC AAGTCTCATC AAGACGATCT ACCCGAGCAA TAATCTACAG GAAATCAAAT
      361 ACCTTCCCAA GAAGGTTAAA GATGCAGTCA AAAGATTCAG GACTAACTGC ATCAAGAACA
      421 CAGAGAAAGA TATATTTCTC AAGATCAGAA GTACTATTCC AGTATGGACG ATTCAAGGCT
      481 TGCTTCACAA ACCAAGGCAA GTAATAGAGA TTGGAGTCTC TAAAAAGGTA GTTCCCACTG
      541 AATCAAAGGC CATGGAGTCA AAGATTCAAA TAGAGGACCT AACAGAACTC GCCGTAAAGA
      601 CTGGCGAACA GTTCATACAG AGTCTCTTAC GACTCAATGA CAAGAAGAAA ATCTTCGTCA
      661 ACATGGTGGA GCACGACACA CTTGTCTACT CCAAAAATAT CAAAGATACA GTCTCAGTAG
      721 ACCAAAGGGC AATTGAGACT TTTCAACAAA GGGTAATATC CGGAAACCTC CTCGGATTCC
      781 ATTGCCCAGC TATCTGTCAC TTTATTGTGA AGATAGTGGA AAAGGAAGGT GGCTCCTACA
      841 AATGCCATCA TTGCGATAAA GGAAAGGCCA TCGTTGAAGA TGCCTCTGCC GACAGTGGTC
      901 CCAAAGATGG ACCCCCACCC ACGAGGAGCA TCGTGGAAAA AGAAAACGTT CCAACCACGT
      961 CTACAAAGCA AGTGGATTGA TGTGATATCT CCACTGACGT AAGGGATGAC GCACAATCCC
     1021 ACTATCCTTC GCAAGACCCT TCCTCTATAT AAGGAAGTTC ATTTCATTTG GAGAGAACAC
     1081 GGGGGACTCT AGAGGATCCA ACATCACCAA TGTgagaccC TGCAGTCCGG CAAAAAAGGG
     1141 CAAGGTGTCA CCACCCTGCC CTTTTTCTTT AAAACCGAAA AGATTACTTC GCGTTATGCA
     1201 GGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC
     1261 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT
     1321 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
     1381 CCACAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     1441 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1501 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1561 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1621 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1681 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     1741 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     1801 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     1861 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     1921 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
     1981 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
     2041 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
     2101 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGCTCG AGGCTTGGAT TCTCACCAAT
     2161 AAAAAACGCC CGGCGGCAAC CGAGCGTTCT GAACAAATCC AGATGGAGTT CTGAGGTCAT
     2221 TACTGGATCT ATCAACAGGA GTCCAAGCGA GCTCGATATC AAATTACGCC CCGCCCTGCC
     2281 ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA
     2341 CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT
     2401 TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC
     2461 TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG
     2521 GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT
     2581 GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA
     2641 AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA
     2701 TACGAAATTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA
     2761 ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT
     2821 GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT
     2881 GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CAT
//