LOCUS       3TERM_Nos-35S           2534 bp ds-DNA     circular     04-NOV-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-E06" "Type.plasmid:L0"          
            "Description" "Use:3TERM after CDS/ CDS12-CTAG" "Plasmid size,
            bp:2534" "Bacteria resistance:Cm" "Plant resistance"
            "Phytozome.ID.v3" "Reference:Sauret-Gueto S, Frangedakis E, et al
            (in preparation)" "Primary
            publication:https\://doi.org/10.1111/nph.15625"
            "Registry.ID.JHLab:HMO_p0_o004" "Name.JHLab"
FEATURES             Location/Qualifiers
     misc_feature    720..723
                     /label="L0 fusion site CGCT"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    247..719
                     /label="3Term_Nos-35S"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(1875..2534)
                     /label="cat / CmR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    243..246
                     /label="3TERM overhang"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(725..730)
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     None            247..500
                     /label="nosT (MoClo)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      516..719
                     /label="35ST (MoClo)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Replication_ori complement(989..1577)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    236..241
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Terminator      complement(1760..1854)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCGGTCT
      241 CAgcttgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
      301 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
      361 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
      421 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
      481 tcatctatgt tactagatcg atccgtatcg atagcctcta gctagagtcg atcgacaagc
      541 tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata
      601 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
      661 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc
      721 gctCGAGACC CTGCAGTCCG GCAAAAAAGG GCAAGGTGTC ACCACCCTGC CCTTTTTCTT
      781 TAAAACCGAA AAGATTACTT CGCGTTATGC AGGCTTCCTC GCTCACTGAC TCGCTGCGCT
      841 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA
      901 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA
      961 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCACAGGCT CCGCCCCCCT GACGAGCATC
     1021 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
     1081 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT
     1141 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT
     1201 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC
     1261 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG
     1321 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG
     1381 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG
     1441 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG
     1501 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA
     1561 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA
     1621 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA
     1681 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT
     1741 CTGACAGCTC GAGGCTTGGA TTCTCACCAA TAAAAAACGC CCGGCGGCAA CCGAGCGTTC
     1801 TGAACAAATC CAGATGGAGT TCTGAGGTCA TTACTGGATC TATCAACAGG AGTCCAAGCG
     1861 AGCTCGATAT CAAATTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA
     1921 AGCATTCTGC CGACATGGAA GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC
     1981 ATCAGCACCT TGTCGCCTTG CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG
     2041 TTGTCCATAT TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG
     2101 ACGAAAAACA TATTCTCAAT AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC
     2161 GCCACATCTT GCGAATATAT GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG
     2221 AGCGATGAAA ACGTTTCAGT TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC
     2281 CATATCACCA GCTCACCGTC TTTCATTGCC ATACGAAATT CCGGATGAGC ATTCATCAGG
     2341 CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT
     2401 AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA
     2461 AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG
     2521 ATTTTTTTCT CCAT
//