LOCUS       3TERM_MpNOP1            3174 bp ds-DNA     circular     04-NOV-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-E09" "Type.plasmid:L0"          
            "Description" "Use:3TERM after CDS/ CDS12-CTAG" "Plasmid size,
            bp:3174" "Bacteria resistance:Cm" "Plant resistance"
            "Phytozome.ID.v3:NOP1 Mapoly0008s0036" "Reference:Sauret-Gueto S,
            Frangedakis E, et al (in preparation)" "Primary
            publication:https\://doi.org/10.1105/tpc.113.117051"
            "Registry.ID.JHLab:HMO_p0_o976" "Name.JHLab"
FEATURES             Location/Qualifiers
     misc_feature    1360..1363
                     /label="L0 fusion site CGCT"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    243..246
                     /label="L0 fusion site GCTT"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Terminator      complement(2400..2494)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1365..1370)
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Replication_ori complement(1629..2217)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1311..1311
                     /label="domestication A to G"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    247..1359
                     /label="3TERM MpNOP1 Tak"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(2515..3174)
                     /label="cat / CmR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    236..241
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      1110..1359
                     /label="terminator"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     utr3            247..1109
                     /label="3UTR_MpNOP1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct
      241 cGGCTTgagt gcagggcaga gttgtggtcc ttccactttc aaatccagcc gaggctggtc
      301 tgaagtttaa gcagcaaggt gcgccaactt gcacctttta taacagcggt ggcagacgcg
      361 tgccaccggc cgcaagcctg cctgcctgcc aagccggaat tgttctgatg cgttgtactt
      421 ggattgattc ctttcatacc gacgaagcct ggtgaaagtg atgttgatga attggacgag
      481 ggctccacga cttctcggtg taacatgtca aaatacgagt tgaacctgaa cagcccagag
      541 tcggtcttgg cgacggatgc gccctcggag cagcagcagg cgagtttggt cgatcgccgg
      601 gttcatcggt tagggtttgt tagattatct gtgattacga atttaaaact taactccata
      661 ggtgatagaa acatcaaaag atttagcagt gagactaggc ccaaccccaa ccccatgtat
      721 ttacaccgag cattatacaa aaagctcctc acatcgagat ggggtcaagt ggggctgacc
      781 tttctgccgg tagccgggct ggagtcgtcg aatttgcgag ccagtagcgt ccatctgatt
      841 gaccatgatc tgatctccga ctctcaagac gtcccctacc catataagct tacaggtttt
      901 tttagatgta agacttgatt atatgtcagc tccatgtcaa cgaaggtgct ctcccctcca
      961 tctccctctt tttaaatgaa gcatattctt taagcgtatg tcagtcaaaa tcctccattt
     1021 cgaagcttgg aaagctttgc gatttgtaac cgtcggctct gagcctgttc agttgaatat
     1081 tcagactacc agcccaggct ttctgtgcag gttctgttca gaatttgtgc accgtgtgtg
     1141 tcatgtgtgt gtgtgtttgt ttggccttaa ggcagaactc tttggcgcac gttgtcgctt
     1201 gcaaaattgc acgtcgacgt tgtatctgtg gaccgtctca cgcagagaac atgcttacga
     1261 gaacagtgat ctcgagccag cttacggacg gggctggtcg ctccagtgga Ggagcaaggg
     1321 ctctatccac tcggatccgc ttcgtcaaca agtgagcgtC GCTTgagacc CTGCAGTCCG
     1381 GCAAAAAAGG GCAAGGTGTC ACCACCCTGC CCTTTTTCTT TAAAACCGAA AAGATTACTT
     1441 CGCGTTATGC AGGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA
     1501 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA
     1561 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
     1621 CTGGCGTTTT TCCACAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT
     1681 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC
     1741 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT
     1801 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC
     1861 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA
     1921 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA
     1981 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG
     2041 TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG
     2101 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT
     2161 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA
     2221 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     2281 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     2341 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGCTC GAGGCTTGGA
     2401 TTCTCACCAA TAAAAAACGC CCGGCGGCAA CCGAGCGTTC TGAACAAATC CAGATGGAGT
     2461 TCTGAGGTCA TTACTGGATC TATCAACAGG AGTCCAAGCG AGCTCGATAT CAAATTACGC
     2521 CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA
     2581 GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG
     2641 CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT
     2701 TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT
     2761 AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT
     2821 GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT
     2881 TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC
     2941 TTTCATTGCC ATACGAAATT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA
     3001 GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG
     3061 CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT
     3121 ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCAT
//