LOCUS       PROM5_MpU6              4069 bp ds-DNA     circular     04-NOV-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-E10" "Type.plasmid:L0"          
            "Description" "Use:Mp U6 promoter (type III RNA polymerase
            promoter) to drive expression of gRNA for CRISPR/Cas9" "Plasmid
            size, bp:4069" "Bacteria resistance:Cm" "Plant resistance"
            "Phytozome.ID.v3" "Reference:Sauret-Gueto S, Frangedakis E, et al
            (in preparation)" "Primary
            publication:https\://doi.org/10.1093/pcp/pcu014"
            "Registry.ID.JHLab:HMO_p0_o981" "Name.JHLab"
FEATURES             Location/Qualifiers
     misc_feature    243..246
                     /label="L0 fusion site GGAG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(3410..4069)
                     /label="cat / CmR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(2260..2265)
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2255..2258
                     /label="TCCG fusion site "
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    236..241
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Replication_ori complement(2524..3112)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Terminator      complement(3295..3389)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    247..2258
                     /label="p5-MpU6 Sugano"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct
      241 cGGGAGTATT CATTCAAAAG AGATTTTTAA AGATCAATTT TTCTTTCTCA TAAGTCTAAT
      301 ACGTCATGAT AATAACTTTA AAAAAAATTG AACTCGTTAA AAAATTTGAT CAAATTTTAA
      361 TTTATTATTA GATCATATAT TACATATCAG TTTCATAATG TTATACTAAG ATCTTTCATA
      421 TATATTTTAA CCCACGAGAC AAAATATGTT TATATCTTTA TTTATTTAAA TATTTTTTAG
      481 CTCAAAACAT CGCAAACTCA GCTATCATAA TGCTTTTTAA TGAAAAAAAC AAATTTTCCT
      541 ATCATACTCT CATTGTTTAT AATTTTTTAG AAAAAAAACT TGAAGATAAA ATAACAACAA
      601 TAATAGTTTC AAAAGCACAT TTTTGTTGTA AATTTCAAGA AAAATACAAT CATATTACTG
      661 ATTGACTACA AAAATTTGTA AATTAATCCA GGCTACATGT TTAAGATGTG TCAAAACTGC
      721 ATCATCTACA TCTCATTTTA ATTTTTCTAA ACTACATACA TAGTATCAAT AACAATTGAA
      781 CAATGCTAGT TAATGTGTTA TACTCATTAG CTTGTAATAT ATCTTCAACT TGATGAATCG
      841 GATTGATCAT AAAATTTCAC AAACTTATGT GCTGATTAAT AAAAAATATA ATCAGATTTT
      901 TCATGAATAA TTAAGATAAA TAACACATGC AAGTCTCAAT TAGAATTTAA CAATCTGACT
      961 ATAAAAAGTC GGTTTATCTT GATTTTCACA AAAATTTAAC ATAGTTTCAA GGTGAATGGG
     1021 CTTTTATTTT AATCCAATCA ACTATTTGAC ACATACATCT TGCAAATTTA TTTATTTTTT
     1081 ATGGAAAAAA ACATGATTTA TGTTTTTCTT TGAGAAAGTA TATTGTGGAT TGTCAAAAAA
     1141 AACCTATGGA AGATTTATAT GTAAGAGTTA AAAATTTATT TTCAAATATG TCAACAATCC
     1201 ACAATATCGA AGAAAATTTT CAAAAAATCA TCACATATGA TGTAAATATA ATATGAATAT
     1261 GTGCAATCTT ATTTCATTTT ACTCTGAAAA CTTAATTTCT TTTTATAGAG CATTCTGTAC
     1321 TTACTGGTGT CAAATATACA GATAAAATGC AGTCCATAAC GTATCACTCT ATAAGCAAAA
     1381 TCATAAACTT CGTGATCTCT CACAGTTCAA ACGAGAACTA TAGAAACCCT TCAACTAGCA
     1441 AAAAACTCAC AACTTTCTAT TCCAATATCT TTAATTGTCT GACGATTTAC AAATAGAAAC
     1501 GAAAACCAGA CACACACACA CACGCAAACT ACAAAGTAAA AACACAACCA CCTGACATTA
     1561 GACAGTGAGA TTATTAAACA TCCTAAACTA AAAAACAAAC AAATTAATTC TCTATGTACA
     1621 AGTCCCCATC GTGATCAATT TTTGCATCCC AATATAGTGG CGGTTAGTAT AAGAGGACCT
     1681 TCGTCTATTC ACTCTCCATG TTTGTCAAAC TATAGTACAG TACATATATG TATGGGAACA
     1741 AATTCCGAAC ACTCTAGTAC ACTCCTCAAC GCAAGTTCTA TCTCATATCT CTGCAAGAGC
     1801 TTATTCCAAG AAACTTTCTT GTGCTTCTAT GCATGCCCAG GTACCTTAAG TCGCGCGCAT
     1861 AGTCACTTCA CAGCCTTTTC AGTTCTCCCT TTGATGGCTC AGAGTGACGC AACTGCACAA
     1921 AAATAAGAAA AAAGTCGAAG CGTCACGAGA GATGTATCCG GCTAGTTTCC TTGGAGTTCG
     1981 GATTGCTCTC TTTCTCTGTC TCCCCGAACC ATATGGAATT TATGAACTGC TGTTCACTAG
     2041 TCAGTCACTT GTTTCAGAAC TTTTGAGTGG TCGATTTATC CACCTTCTTG TTTCTTCCTG
     2101 GGTCCAGAAT CTGACTTCTT GAGACAACAA ACAGTGCACA GTGGATCGAA GAATGACCAC
     2161 AGTTGAGACA CGTGGCGCTG GCCTCATTTA TTGCACCATA CGTTTAGTGA AGAACTGAAT
     2221 AATGGTGGAT ATAAAGCAGA GTTGCACCCA GCCTCTCGTg agaccCTGCA GTCCGGCAAA
     2281 AAAGGGCAAG GTGTCACCAC CCTGCCCTTT TTCTTTAAAA CCGAAAAGAT TACTTCGCGT
     2341 TATGCAGGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT
     2401 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
     2461 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC
     2521 GTTTTTCCAC AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     2581 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     2641 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     2701 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     2761 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     2821 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     2881 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     2941 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     3001 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     3061 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
     3121 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
     3181 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
     3241 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGCTCGAGGC TTGGATTCTC
     3301 ACCAATAAAA AACGCCCGGC GGCAACCGAG CGTTCTGAAC AAATCCAGAT GGAGTTCTGA
     3361 GGTCATTACT GGATCTATCA ACAGGAGTCC AAGCGAGCTC GATATCAAAT TACGCCCCGC
     3421 CCTGCCACTC ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT
     3481 CACAAACGGC ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG CCTTGCGTAT
     3541 AATATTTGCC CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC ACGTTTAAAT
     3601 CAAAACTGGT GAAACTCACC CAGGGATTGG CTGAGACGAA AAACATATTC TCAATAAACC
     3661 CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA TATATGTGTA
     3721 GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT TCAGTTTGCT
     3781 CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA CCGTCTTTCA
     3841 TTGCCATACG AAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG
     3901 GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA
     3961 CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT
     4021 GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCAT
//