LOCUS       L1_KanR-Ck1             9123 bp ds-DNA     circular     18-NOV-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-F01" "Type.plasmid:L1"          
            "Description:p5-MpEF1a\:nptII_3t35S" "Use:L1 in position 1 (next to
            LB) for KanR" "Plasmid size, bp:9123" "Bacteria resistance:Kan"
            "Plant resistance" "Phytozome.ID.v3:EF1a Mapoly0024s0116"
            "Reference:Sauret-Gueto S, Frangedakis E, et al (in preparation)"
            "Primary publication:this study" "Registry.ID.JHLab:HM_L1_o128"
            "Name.JHLab:L1_030-Ck1"
FEATURES             Location/Qualifiers
     misc_feature    6768..6962
                     /label="pVS1 oriV origin of replication"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3225..3228
                     /label="L0 fusion site CGCT"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2909..2915
                     /label="SapI site removed"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    7632..8220
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      3021..3224
                     /label="3Term_35S (MoClo)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4571..5200
                     /label="pVS1 StaA stability protein"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(3234..3240)
                     /label="SapI recog site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1822..1828
                     /label="SapI mut"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4332..5332
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(8308..9123)
                     /label="nptI / KanR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1439..1445
                     /label="TATA box"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5629..6702
                     /label="pVS1 RepA  replication protein"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3230..3232
                     /label="Loop b fusion site GCA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3017..3020
                     /label="L0 fusion site GCTT"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2205..2217
                     /label="exon 5UTR_MpEF1a"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    484..1485
                     /label="PROM_MpEF1a"
                     /ApEinfo_revcolor=#f96381
                     /ApEinfo_fwdcolor=#f96381
     misc_feature    2699..2705
                     /label="SapI site removed"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    480..483
                     /label="L0 fusion site GGAG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1845..2204
                     /label=" Intron in 5UTR_MpEF1a"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    7306..7446
                     /label="basis of mobility region from pBR322"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    476..478
                     /label="Loop a fusion site ATG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1486..1844
                     /label="exon 5UTR_MpEF1a"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3246..3270
                     /label="RB"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    468..474
                     /label="SapI recog site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    484..2217
                     /label="PROM5_MpEF1a"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2036..2041
                     /label="BsaI mut, like Guru"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2218..2221
                     /label="L0 fusion site AATG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     gene            2219..3016
                     /label="nptII / KanR"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    433..458
                     /label="LB"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
       61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
      121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
      181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
      241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
      301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
      361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
      421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcaatgag
      481 gagcaaatga gtcacacaca ttgttgagag acatatcaaa ataactcaat tgtaaggtaa
      541 gttgatagat ttcccagtta aatgcattat tttaacaata tttgtatctc aaattttcaa
      601 aatcatatca aatttgcaga tcttgatgat gattcacaac tctgtcttcg atttttgatt
      661 gttaacgggt atcgatattt gggtaactta aaaagaattt gctaaagatc ttaaaaaaca
      721 gattaaaaaa acctttcaaa atagctaatc ctttaaatcc gtttcacaat ataatgctca
      781 ttgatgataa tcgaaaagat tccattttaa agtgcatata aattgcttaa ttatctcttt
      841 ctgtctcgta atattttgtt ctctcctatt ggatcggacc cgcatcttct cgatcagttc
      901 actatactaa tatttggtca acttcaagag agattagaac ttcgttctcc aaagtgtaaa
      961 ggccgacaag cagaaaagga aacttcaatc agaagccttc taggttttcc cgcgtcccaa
     1021 aaagcgaagg gaggacgggg aggacggtga ggccagaaga agaaagcaac agtaaaatct
     1081 catgtgattg cgtgcatgtc atggatctat gggatgctag tccaccgttt ggaagtatgt
     1141 gcagaaggag aaaggcgcgg gaggccaaaa gcgacttcgg ccacctctct actttctaga
     1201 aagtttgctc gcagcttgta ataagcgcgg aaaaggtact cgaaaaaccg gagcaatcgg
     1261 tgtggtatca gcagtcgggt tcgctaaacc tacgtcttct tattttggaa tgttaaattg
     1321 aaagggcgtg ccggccctaa gcacggacga tgtgacagac ggaggagtgt gagcaattcg
     1381 gatgacgatg ctggccggca gtcacgacaa agagtccgaa ggggcatgga cagggcaata
     1441 tataaatgga aaagaggctt tgaatattcc taacccttcg tcgtcggcct cccaacttgc
     1501 cctcgcatgc tgaattggcc gcctatctgc gaatggccgg ccgccgaccc taagtcgcaa
     1561 taacttgaac ggagccgatg caaagtcctt atcgaacgct cgttattata aacggggcag
     1621 acctaggtat ttaattatat caaaaccctc cgcaatttcg ctaatcattc cctaatttct
     1681 tctactttgg cgtctagtgc tgtggtcgcg cttgctctca aagctcgctg ggaggcagtg
     1741 gagcgtctgg cttaaaatcg atcgccgcaa ccctagagtt cctttcactt ctttctctct
     1801 tgcgtggaga gcatcgacat cgaggagctg catcttgtct tctggtatgc gccttgtgcc
     1861 ctcgtctctg tacgtcgtta cgattgctcg atattgtgat tgtctagacc tgaaacccga
     1921 ctggagcttt tacgtggtgt cgattggggc ctcgagcgag tgatttttta ggttcgtttt
     1981 agccaagtac atcccacttt ggtcagtcct gttatctcgg taaggtgttc agttcggtca
     2041 ctggtgcgga ggtgcctcgt aagtttcctg catgatagat ttgtctatcc ttgacttgaa
     2101 gaggagtcga aggtcagcga ggttggctgt gtgctatgga cgtcggtgct ccttggttcc
     2161 gtgcttctat gggtgttgcc tcaacagtat tgatgtgcct gcagaaaggt tgtcaccaat
     2221 ggggattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     2281 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt ttcggctgtc
     2341 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     2401 ccaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     2461 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     2521 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     2581 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     2641 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     2701 agAAcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     2761 cggcgaggat ctcgtcgtga cacatggcga tgcctgcttg ccgaatatca tggtggaaaa
     2821 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     2881 catagcgttg gctacccgtg atattgctga agAActtggc ggcgaatggg ctgaccgctt
     2941 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     3001 tgacgagttc ttctgagctt ctctagctag agtcgatcga caagctcgag tttctccata
     3061 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt
     3121 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
     3181 aatttctaat tcctaaaaCc aaaatccagt actaaaatcc agatcgctag catgaagagc
     3241 aattctgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
     3301 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
     3361 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
     3421 gtcggcttct gacgttcagt gcagccgtca tctgaaaacg acatgtcgca caagtcctaa
     3481 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
     3541 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
     3601 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
     3661 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
     3721 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
     3781 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
     3841 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
     3901 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
     3961 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
     4021 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
     4081 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
     4141 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
     4201 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
     4261 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
     4321 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ctctcaaccg
     4381 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
     4441 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
     4501 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
     4561 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
     4621 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
     4681 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
     4741 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
     4801 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
     4861 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
     4921 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
     4981 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
     5041 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
     5101 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
     5161 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
     5221 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
     5281 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
     5341 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
     5401 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
     5461 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
     5521 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc
     5581 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc
     5641 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg
     5701 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg
     5761 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg
     5821 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc
     5881 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg
     5941 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag
     6001 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg
     6061 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc
     6121 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga
     6181 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg
     6241 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg
     6301 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag
     6361 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt
     6421 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc
     6481 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc
     6541 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac
     6601 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc
     6661 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta
     6721 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt ggatagcacg
     6781 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag
     6841 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat
     6901 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca
     6961 taactgtctg gccagcgcac agccgaagtg ctgcaaaaag cgcctaccct tcggtcgctg
     7021 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg
     7081 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac
     7141 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     7201 ctgacacatg cagctcccgg tgacggtcac agcttgtctg taagcggatg ccgggagcag
     7261 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     7321 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     7381 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     7441 atcaggcgct catccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7501 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7561 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7621 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7681 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7741 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7801 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7861 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7921 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7981 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8041 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8101 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8161 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8221 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8281 gggattttgg tcatgcattc taggtgatta gaaaaactca tcgagcatca aatgaaactg
     8341 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     8401 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     8461 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     8521 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat
     8581 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     8641 aaccaaaccg ttattcattc gtgattgcgc ctgagcgagt cgaaatacgc gatcgctgtt
     8701 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     8761 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttccctgg
     8821 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     8881 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     8941 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg
     9001 gtagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     9061 agcatccatg ttggaattta atcgcggcct tgagcaagac gtttcccgtt gaatatggct
     9121 cat
//