LOCUS       L1_HyR-Ck1              8479 bp ds-DNA     circular     04-NOV-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-F03" "Type.plasmid:L1"          
            "Description:p5-35S\:hptII_3t35S" "Use:L1 in position 1 (next to
            LB) for HygR" "Plasmid size, bp:8479" "Bacteria resistance:Kan"
            "Plant resistance" "Phytozome.ID.v3" "Reference:Sauret-Gueto S,
            Frangedakis E, et al (in preparation)" "Primary publication:this
            study" "Registry.ID.JHLab:HM_L1_o072" "Name.JHLab:L1_071-Ck1"
FEATURES             Location/Qualifiers
     terminator      2377..2580
                     /label="3Term_35S (MoClo)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    468..474
                     /label="SapI recog site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    484..1318
                     /label="p5-35S"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    6662..6802
                     /label="basis of mobility region from pBR322"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    476..478
                     /label="Loop a fusion site ATG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1347..2372
                     /label="hptII / HygR (seq a bit diff from MoClo, but same AA, except Q>K)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    433..458
                     /label="LB"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4985..6058
                     /label="pVS1 RepA  replication protein"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2602..2626
                     /label="RB"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3927..4556
                     /label="pVS1 StaA stability protein"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    6988..7576
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2586..2588
                     /label="Loop b fusion site GCA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    6124..6318
                     /label="pVS1 oriV origin of replication"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1346..1349
                     /label="L0 fusion site AATG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    480..483
                     /label="L0 fusion site GGAG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2590..2596)
                     /label="SapI recog site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2581..2584
                     /label="L0 fusion site CGCT"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2373..2376
                     /label="L0 fusion site GCTT"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        1267..1312
                     /label="min 35S pro"
                     /ApEinfo_revcolor=#5573ff
                     /ApEinfo_fwdcolor=#5573ff
     misc_feature    3688..4688
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1283..1289
                     /label="TATA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(7664..8479)
                     /label="nptI / KanR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
       61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
      121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
      181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
      241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
      301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
      361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
      421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcaatgag
      481 gagAGATTAG CCTTTTCAAT TTCAGAAAGA ATGCTAACCC ACAGATGGTT AGAGAGGCTT
      541 ACGCAGCAAG TCTCATCAAG ACGATCTACC CGAGCAATAA TCTACAGGAA ATCAAATACC
      601 TTCCCAAGAA GGTTAAAGAT GCAGTCAAAA GATTCAGGAC TAACTGCATC AAGAACACAG
      661 AGAAAGATAT ATTTCTCAAG ATCAGAAGTA CTATTCCAGT ATGGACGATT CAAGGCTTGC
      721 TTCACAAACC AAGGCAAGTA ATAGAGATTG GAGTCTCTAA AAAGGTAGTT CCCACTGAAT
      781 CAAAGGCCAT GGAGTCAAAG ATTCAAATAG AGGACCTAAC AGAACTCGCC GTAAAGACTG
      841 GCGAACAGTT CATACAGAGT CTCTTACGAC TCAATGACAA GAAGAAAATC TTCGTCAACA
      901 TGGTGGAGCA CGACACACTT GTCTACTCCA AAAATATCAA AGATACAGTC TCAGTAGACC
      961 AAAGGGCAAT TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATTCCATT
     1021 GCCCAGCTAT CTGTCACTTT ATTGTGAAGA TAGTGGAAAA GGAAGGTGGC TCCTACAAAT
     1081 GCCATCATTG CGATAAAGGA AAGGCCATCG TTGAAGATGC CTCTGCCGAC AGTGGTCCCA
     1141 AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AAACGTTCCA ACCACGTCTA
     1201 CAAAGCAAGT GGATTGATGT GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT
     1261 ATCCTTCGCA AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGAACACGGG
     1321 GGACTCTAGA GGATCCAACA TCACCAATGa aaaagcctga actcaccgcg acgtctgtcg
     1381 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     1441 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     1501 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     1561 tcccgattcc ggaagtgctt gacattgggg agtttagcga gagcctgacc tattgcatct
     1621 cccgccgttc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     1681 tacaaccggt cgcggaggct atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     1741 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     1801 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     1861 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     1921 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     1981 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     2041 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     2101 ggcatccgga gcttgcagga tcgccacgac tccgggcgta tatgctccgc attggtcttg
     2161 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     2221 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     2281 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     2341 gccccagcac tcgtccgagg gcaaagaaat agGCTTctct agctagagtc gatcgacaag
     2401 ctcgagtttc tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat
     2461 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
     2521 atacttctat caataaaatt tctaattcct aaaaCcaaaa tccagtacta aaatccagat
     2581 cgctagcatg aagagcaatt ctgacaggat atattggcgg gtaaacctaa gagaaaagag
     2641 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
     2701 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct
     2761 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcatctg aaaacgacat
     2821 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct
     2881 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc
     2941 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca
     3001 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga
     3061 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg
     3121 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct
     3181 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc
     3241 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc
     3301 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc
     3361 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg
     3421 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca
     3481 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag
     3541 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga
     3601 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt
     3661 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc
     3721 gcgcacctct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg
     3781 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga
     3841 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta
     3901 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg
     3961 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg
     4021 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg
     4081 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg
     4141 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg
     4201 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca
     4261 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa
     4321 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg
     4381 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg
     4441 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg
     4501 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta
     4561 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc
     4621 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
     4681 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
     4741 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa
     4801 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac
     4861 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg
     4921 taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat
     4981 cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga
     5041 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc
     5101 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc
     5161 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt
     5221 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc
     5281 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc
     5341 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga
     5401 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa
     5461 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg
     5521 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac
     5581 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc
     5641 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga
     5701 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc
     5761 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct
     5821 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat
     5881 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct
     5941 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc
     6001 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg
     6061 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggactctt
     6121 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta
     6181 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa
     6241 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac
     6301 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagtgctgc aaaaagcgcc
     6361 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc
     6421 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc
     6481 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg
     6541 atgacggtga aaacctctga cacatgcagc tcccggtgac ggtcacagct tgtctgtaag
     6601 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     6661 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
     6721 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     6781 aaggagaaaa taccgcatca ggcgctcatc cgcttcctcg ctcactgact cgctgcgctc
     6841 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6901 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6961 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7021 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7081 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7141 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7201 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7261 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7321 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7381 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     7441 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7501 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7621 cgaaaactca cgttaaggga ttttggtcat gcattctagg tgattagaaa aactcatcga
     7681 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     7741 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     7801 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     7861 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     7921 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     7981 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagtcgaa
     8041 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     8101 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     8161 atgctgtttt ccctgggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     8221 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     8281 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     8341 gcttcccata caatcggtag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     8401 tatacccata taaatcagca tccatgttgg aatttaatcg cggccttgag caagacgttt
     8461 cccgttgaat atggctcat
//