LOCUS       L1_Cas9-Ck4_           12750 bp ds-DNA     circular     27-MAR-2020
DEFINITION  .
KEYWORDS    "Addgene:submission in process" "OP.kit.position:Plate01-G01"      
            "Type.plasmid:L1" "Description:p5-MpEF1a\:Cas9_3tNos-35S" "Use:L1
            in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR
            vectors" "Plasmid size, bp:12750" "Bacteria resistance:Kan" "Plant
            resistance" "Phytozome.ID.v3:EF1a Mapoly0024s0116"
            "Reference:Sauret-Gueto et al. (2020)" "Primary publication:this
            study" "Registry.ID.JHLab:HM_L1_o273" "Name.JHLab:L1_404-Ck4"
FEATURES             Location/Qualifiers
     polyA_site      6379..6851
                     /label="3Term_Nos-35S"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    6857..6859
                     /label="Loop o fusion site GGT"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1845..2204
                     /label=" Intron in 5UTR_MpEF1a"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    468..474
                     /label="SapI recog site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    8198..8827
                     /label="pVS1 StaA stability protein"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    6826..6826
                     /label="mut C to T"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             2219..6358
                     /label="AtcoCas9 (Puchta)STOP"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    476..478
                     /label="Loop e fusion site CAG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    480..483
                     /label="L0 fusion site GGAG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1832..1835
                     /label="L0 fusion site TACT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    6852..6855
                     /label="L0 fusion site CGCT"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    6873..6897
                     /label="RB"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2205..2217
                     /label="exon 5UTR_MpEF1a"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(6861..6867)
                     /label="SapI recog site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    6379..6632
                     /label="nosT (MoClo)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    10395..10589
                     /label="pVS1 oriV origin of replication"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    6335..6355
                     /label="NLS_SV40"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    11259..11847
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    10933..11073
                     /label="basis of mobility region from pBR322"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1486..2217
                     /label="5UTR_MpEF1a"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1486..1844
                     /label="exon 5UTR_MpEF1a"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(11935..12750)
                     /label="nptI / KanR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      6648..6851
                     /label="35ST (MoClo)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    484..1485
                     /label="PROM_MpEF1a"
                     /ApEinfo_revcolor=#f96381
                     /ApEinfo_fwdcolor=#f96381
     misc_feature    2218..2221
                     /label="L0 fusion site AATG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    433..458
                     /label="LB"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    9256..10329
                     /label="pVS1 RepA  replication protein"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    6359..6362
                     /label="L0 fusion site GCTT"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2036..2041
                     /label="BsaI mut"
                     /ApEinfo_revcolor=#f96381
                     /ApEinfo_fwdcolor=#f96381
     misc_feature    7959..8959
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
       61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
      121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
      181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
      241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
      301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
      361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
      421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcacagag
      481 gagcaaatga gtcacacaca ttgttgagag acatatcaaa ataactcaat tgtaaggtaa
      541 gttgatagat ttcccagtta aatgcattat tttaacaata tttgtatctc aaattttcaa
      601 aatcatatca aatttgcaga tcttgatgat gattcacaac tctgtcttcg atttttgatt
      661 gttaacgggt atcgatattt gggtaactta aaaagaattt gctaaagatc ttaaaaaaca
      721 gattaaaaaa acctttcaaa atagctaatc ctttaaatcc gtttcacaat ataatgctca
      781 ttgatgataa tcgaaaagat tccattttaa agtgcatata aattgcttaa ttatctcttt
      841 ctgtctcgta atattttgtt ctctcctatt ggatcggacc cgcatcttct cgatcagttc
      901 actatactaa tatttggtca acttcaagag agattagaac ttcgttctcc aaagtgtaaa
      961 ggccgacaag cagaaaagga aacttcaatc agaagccttc taggttttcc cgcgtcccaa
     1021 aaagcgaagg gaggacgggg aggacggtga ggccagaaga agaaagcaac agtaaaatct
     1081 catgtgattg cgtgcatgtc atggatctat gggatgctag tccaccgttt ggaagtatgt
     1141 gcagaaggag aaaggcgcgg gaggccaaaa gcgacttcgg ccacctctct actttctaga
     1201 aagtttgctc gcagcttgta ataagcgcgg aaaaggtact cgaaaaaccg gagcaatcgg
     1261 tgtggtatca gcagtcgggt tcgctaaacc tacgtcttct tattttggaa tgttaaattg
     1321 aaagggcgtg ccggccctaa gcacggacga tgtgacagac ggaggagtgt gagcaattcg
     1381 gatgacgatg ctggccggca gtcacgacaa agagtccgaa ggggcatgga cagggcaata
     1441 tataaatgga aaagaggctt tgaatattcc taacccttcg tcgtcggcct cccaacttgc
     1501 cctcgcatgc tgaattggcc gcctatctgc gaatggccgg ccgccgaccc taagtcgcaa
     1561 taacttgaac ggagccgatg caaagtcctt atcgaacgct cgttattata aacggggcag
     1621 acctaggtat ttaattatat caaaaccctc cgcaatttcg ctaatcattc cctaatttct
     1681 tctactttgg cgtctagtgc tgtggtcgcg cttgctctca aagctcgctg ggaggcagtg
     1741 gagcgtctgg cttaaaatcg atcgccgcaa ccctagagtt cctttcactt ctttctctct
     1801 tgcgtggaga gcatcgacat cgaggagctg cTACTtgtct tctggtatgc gccttgtgcc
     1861 ctcgtctctg tacgtcgtta cgattgctcg atattgtgat tgtctagacc tgaaacccga
     1921 ctggagcttt tacgtggtgt cgattggggc ctcgagcgag tgatttttta ggttcgtttt
     1981 agccaagtac atcccacttt ggtcagtcct gttatctcgg taaggtgttc agttcggtcA
     2041 ctggtgcgga ggtgcctcgt aagtttcctg catgatagat ttgtctatcc ttgacttgaa
     2101 gaggagtcga aggtcagcga ggttggctgt gtgctatgga cgtcggtgct ccttggttcc
     2161 gtgcttctat gggtgttgcc tcaacagtat tgatgtgcct gcagaaaggt tgtcaccaat
     2221 ggataagaag tactctatcg gactcgatat cggaactaac tctgtgggat gggctgtgat
     2281 caccgatgag tacaaggtgc catctaagaa gttcaaggtt ctcggaaaca ccgataggca
     2341 ctctatcaag aaaaacctta tcggtgctct cctcttcgat tctggtgaaa ctgctgaggc
     2401 taccagactc aagagaaccg ctagaagaag gtacaccaga agaaagaaca ggatctgcta
     2461 cctccaagag atcttctcta acgagatggc taaagtggat gattcattct tccacaggct
     2521 cgaagagtca ttcctcgtgg aagaagataa gaagcacgag aggcacccta tcttcggaaa
     2581 catcgttgat gaggtggcat accacgagaa gtaccctact atctaccacc tcagaaagaa
     2641 gctcgttgat tctactgata aggctgatct caggctcatc tacctcgctc tcgctcacat
     2701 gatcaagttc agaggacact tcctcatcga gggtgatctc aaccctgata actctgatgt
     2761 ggataagttg ttcatccagc tcgtgcagac ctacaaccag cttttcgaag agaaccctat
     2821 caacgcttca ggtgtggatg ctaaggctat cctctctgct aggctctcta agtcaagaag
     2881 gcttgagaac ctcattgctc agctccctgg tgagaagaag aacggacttt tcggaaactt
     2941 gatcgctctc tctctcggac tcacccctaa cttcaagtct aacttcgatc tcgctgagga
     3001 tgcaaagctc cagctctcaa aggataccta cgatgatgat ctcgataacc tcctcgctca
     3061 gatcggagat cagtacgctg atttgttcct cgctgctaag aacctctctg atgctatcct
     3121 cctcagtgat atcctcagag tgaacaccga gatcaccaag gctccactct cagcttctat
     3181 gatcaagaga tacgatgagc accaccagga tctcacactt ctcaaggctc ttgttagaca
     3241 gcagctccca gagaagtaca aagagatttt cttcgatcag tctaagaacg gatacgctgg
     3301 ttacatcgat ggtggtgcat ctcaagaaga gttctacaag ttcatcaagc ctatcctcga
     3361 gaagatggat ggaaccgagg aactcctcgt gaagctcaat agagaggatc ttctcagaaa
     3421 gcagaggacc ttcgataacg gatctatccc tcatcagatc cacctcggag agttgcacgc
     3481 tatccttaga aggcaagagg atttctaccc attcctcaag gataacaggg aaaagattga
     3541 gaagattctc accttcagaa tcccttacta cgtgggacct ctcgctagag gaaactcaag
     3601 attcgcttgg atgaccagaa agtctgagga aaccatcacc ccttggaact tcgaagaggt
     3661 ggtggataag ggtgctagtg ctcagtcttt catcgagagg atgaccaact tcgataagaa
     3721 ccttccaaac gagaaggtgc tccctaagca ctctttgctc tacgagtact tcaccgtgta
     3781 caacgagttg accaaggtta agtacgtgac cgagggaatg aggaagcctg cttttttgtc
     3841 aggtgagcaa aagaaggcta tcgttgatct cttgttcaag accaacagaa aggtgaccgt
     3901 gaagcagctc aaagaggatt acttcaagaa aatcgagtgc ttcgattcag ttgagatttc
     3961 tggtgttgag gataggttca acgcatctct cggaacctac cacgatctcc tcaagatcat
     4021 taaggataag gatttcttgg ataacgagga aaacgaggat atcttggagg atatcgttct
     4081 taccctcacc ctctttgaag atagagagat gattgaagaa aggctcaaga cctacgctca
     4141 tctcttcgat gataaggtga tgaagcagtt gaagagaaga agatacactg gttggggaag
     4201 gctctcaaga aagctcatta acggaatcag ggataagcag tctggaaaga caatccttga
     4261 tttcctcaag tctgatggat tcgctaacag aaacttcatg cagctcatcc acgatgattc
     4321 tctcaccttt aaagaggata tccagaaggc tcaggtttca ggacagggtg atagtctcca
     4381 tgagcatatc gctaacctcg ctggatctcc tgcaatcaag aagggaatcc tccagactgt
     4441 gaaggttgtg gatgagttgg tgaaggtgat gggaaggcat aagcctgaga acatcgtgat
     4501 cgaaatggct agagagaacc agaccactca gaagggacag aagaactcta gggaaaggat
     4561 gaagaggatc gaggaaggta tcaaagagct tggatctcag atcctcaaag agcaccctgt
     4621 tgagaacact cagctccaga atgagaagct ctacctctac tacctccaga acggaaggga
     4681 tatgtatgtg gatcaagagt tggatatcaa caggctctct gattacgatg ttgatcatat
     4741 cgtgccacag tcattcttga aggatgattc tatcgataac aaggtgctca ccaggtctga
     4801 taagaacagg ggtaagagtg ataacgtgcc aagtgaagag gttgtgaaga aaatgaagaa
     4861 ctattggagg cagctcctca acgctaagct catcactcag agaaagttcg ataacttgac
     4921 taaggctgag aggggaggac tctctgaatt ggataaggca ggattcatca agaggcagct
     4981 tgtggaaacc aggcagatca ctaagcacgt tgcacagatc ctcgattcta ggatgaacac
     5041 caagtacgat gagaacgata agttgatcag ggaagtgaag gttatcaccc tcaagtcaaa
     5101 gctcgtgtct gatttcagaa aggatttcca attctacaag gtgagggaaa tcaacaacta
     5161 ccaccacgct cacgatgctt accttaacgc tgttgttgga accgctctca tcaagaagta
     5221 tcctaagctc gagtcagagt tcgtgtacgg tgattacaag gtgtacgatg tgaggaagat
     5281 gatcgctaag tctgagcaag agatcggaaa ggctaccgct aagtatttct tctactctaa
     5341 catcatgaat ttcttcaaga ccgagattac cctcgctaac ggtgagatca gaaagaggcc
     5401 actcatcgag acaaacggtg aaacaggtga gatcgtgtgg gataagggaa gggatttcgc
     5461 taccgttaga aaggtgctct ctatgccaca ggtgaacatc gttaagaaaa ccgaggtgca
     5521 gaccggtgga ttctctaaag agtctatcct ccctaagagg aactctgata agctcattgc
     5581 taggaagaag gattgggacc ctaagaaata cggtggtttc gattctccta ccgtggctta
     5641 ctctgttctc gttgtggcta aggttgagaa gggaaagagt aagaagctca agtctgttaa
     5701 ggaacttctc ggaatcacta tcatggaaag gtcatctttc gagaagaacc caatcgattt
     5761 cctcgaggct aagggataca aagaggttaa gaaggatctc atcatcaagc tcccaaagta
     5821 ctcactcttc gaactcgaga acggtagaaa gaggatgctc gcttctgctg gtgagcttca
     5881 aaagggaaac gagcttgctc tcccatctaa gtacgttaac tttctttacc tcgcttctca
     5941 ctacgagaag ttgaagggat ctccagaaga taacgagcag aagcaacttt tcgttgagca
     6001 gcacaagcac tacttggatg agatcatcga gcagatctct gagttctcta aaagggtgat
     6061 cctcgctgat gcaaacctcg ataaggtgtt gtctgcttac aacaagcaca gagataagcc
     6121 tatcagggaa caggcagaga acatcatcca tctcttcacc cttaccaacc tcggtgctcc
     6181 tgctgctttc aagtacttcg atacaaccat cgataggaag agatacacct ctaccaaaga
     6241 agtgctcgat gctaccctca tccatcagtc tatcactgga ctctacgaga ctaggatcga
     6301 tctctcacag ctcggtggtg attcaagggc tgatcctaag aagaagagga aggtttgagc
     6361 ttgagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat
     6421 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
     6481 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     6541 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     6601 tcgcgcgcgg tgtcatctat gttactagat cGATCCGTAT CGATAGCctc tagctagagt
     6661 cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga taagggaatt
     6721 agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
     6781 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaTcaaa atccagtact
     6841 aaaatccaga tCGCTaggtt gaagagcaat tctgacagga tatattggcg ggtaaaccta
     6901 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     6961 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
     7021 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcatct
     7081 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
     7141 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
     7201 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
     7261 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
     7321 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
     7381 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
     7441 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
     7501 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
     7561 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
     7621 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
     7681 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
     7741 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
     7801 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
     7861 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
     7921 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
     7981 tcgagccgcc cgcgcacctc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
     8041 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
     8101 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
     8161 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
     8221 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
     8281 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
     8341 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
     8401 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
     8461 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
     8521 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
     8581 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
     8641 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
     8701 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
     8761 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
     8821 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
     8881 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
     8941 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
     9001 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
     9061 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
     9121 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
     9181 ttggccaggc gtaagcggct gggttgCctg ccggccctgc aatggcactg gaacccccaa
     9241 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
     9301 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
     9361 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
     9421 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
     9481 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
     9541 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
     9601 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
     9661 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
     9721 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
     9781 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
     9841 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
     9901 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
     9961 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
    10021 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
    10081 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
    10141 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
    10201 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
    10261 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
    10321 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
    10381 aaaggactct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
    10441 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
    10501 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
    10561 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagtgctg
    10621 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
    10681 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
    10741 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
    10801 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggtga cggtcacagc
    10861 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    10921 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
    10981 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
    11041 cacagatgcg taaggagaaa ataccgcatc aggcgctcat ccgcttcctc gctcactgac
    11101 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    11161 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    11221 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    11281 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    11341 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    11401 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    11461 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    11521 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    11581 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    11641 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
    11701 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    11761 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    11821 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    11881 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag gtgattagaa
    11941 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
    12001 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
    12061 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
    12121 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
    12181 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
    12241 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
    12301 agcgagtcga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
    12361 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
    12421 taatacctgg aatgctgttt tccctgggat cgcagtggtg agtaaccatg catcatcagg
    12481 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
    12541 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
    12601 tggcgcatcg ggcttcccat acaatcggta gattgtcgca cctgattgcc cgacattatc
    12661 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggccttga
    12721 gcaagacgtt tcccgttgaa tatggctcat
//