LOCUS       L2_NOP1gRNA-Cas9-CsA   17533 bp ds-DNA     circular     11-NOV-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-G05" "Type.plasmid:L2"          
            "Description:p5-35Sx2\:HygR p5-MpU6\:NOP1gRNA1 p5-MpEF1a\:NLS-Cas9"
            "Use:Plasmid with NOP1-gRNA to tranform Mp as CRIPSR control.
            Contains Cas9 and HygR" "Plasmid size, bp:18389" "Bacteria
            resistance:Sm" "Plant resistance:Hyg" "Phytozome.ID.v3:EF1a
            Mapoly0024s0116, NOP1 Mapoly0008s0036" "Reference:Sauret-Gueto S,
            Frangedakis E, et al (in preparation)" "Primary publication:this
            study" "Registry.ID.JHLab:HM_L2_o259" "Name.JHLab:L2_280-CsA"
FEATURES             Location/Qualifiers
     misc_feature    2575..2774
                     /label="Odd-spacer"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    505..829
                     /label="35S repetition 1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2567..2569
                     /label="Loop b fusion site GCA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             6680..10819
                     /label="AtcoCas9 (Puchta)STOP"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4895..4901
                     /label="gRNA-PolTer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    468..468
                     /label="a > C"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    12652..13281
                     /label="pVS1 StaA stability protein"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    481..483
                     /label="Loop a fusion site ATG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      2358..2561
                     /label="3Term_35S (MoClo)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        505..1255
                     /label="p5-35Sx2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5947..6678
                     /label="5UTR_MpEF1a"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    6306..6665
                     /label=" Intron in 5UTR_MpEF1a"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1295..1326
                     /label="this part is not in p5-35Sx2v2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    433..458
                     /label="LB"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      11097..11300
                     /label="35ST (MoClo)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    15713..16301
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    6497..6502
                     /label="BsaI mut"
                     /ApEinfo_revcolor=#f96381
                     /ApEinfo_fwdcolor=#f96381
     promoter        complement(17400..17533)
                     /label="Promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4825..4894
                     /label="gRNA scaffold"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    11275..11275
                     /label="mut C to T"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4797..4799
                     /label="TCG fusion site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5947..6305
                     /label="exon 5UTR_MpEF1a"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    14849..15043
                     /label="pVS1 oriV origin of replication"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     polyA_site      10828..11300
                     /label="3Term_Nos-35S"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    10796..10816
                     /label="NLS_SV40"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2788..4799
                     /label="p5-MpU6 Sugano"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    4800..4818
                     /label="NOP1 target_1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4819..4824
                     /label="gRNA scaffold"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    6666..6678
                     /label="exon 5UTR_MpEF1a"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    476..479
                     /label="Loop A fusion site, GGAG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(16389..17399)
                     /label="aadA-1 / Sm resistance"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    13710..14783
                     /label="pVS1 RepA  replication protein"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    469..474
                     /label="BsaI fusion site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     None            10828..11081
                     /label="nosT (MoClo)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    12413..13413
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(11315..11320)
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    11327..11351
                     /label="RB"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    11306..11308
                     /label="Loop o fusion site GGT"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    830..1154
                     /label="35S repetition 2"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    6293..6296
                     /label="fusion site PROM-5UTR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    4937..4939
                     /label="Loop d fusion site CAG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    11310..11313
                     /label="Loop B fusion site, TACT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    15387..15527
                     /label="basis of mobility region from pBR322"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2780..2782
                     /label="Loop g fusion site TAC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4945..5946
                     /label="PROM_MpEF1a"
                     /ApEinfo_revcolor=#f96381
                     /ApEinfo_fwdcolor=#f96381
     CDS             1328..2353
                     /label="hptII / HygR (seq a bit diff from MoClo, but same AA, except Q>K)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
       61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
      121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
      181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
      241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
      301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
      361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
      421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acggatcCgg tctcaggaga
      481 atgaggaggc tagagcagct tgccaacatg gtggagcacg acactctcgt ctactccaag
      541 aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
      601 atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca
      661 gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt
      721 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
      781 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga
      841 gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc
      901 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
      961 tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca
     1021 ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg
     1081 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
     1141 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
     1201 gcaagaCcct tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca
     1261 ccagtctctc tctacaaatc tatctctctc gagctttcgc agatccgggg ggcaatgaga
     1321 tTCACCAATG aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     1381 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     1441 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     1501 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     1561 tgacattggg gagtttagcg agagcctgac ctattgcatc tcccgccgtt cacagggtgt
     1621 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctacaaccgg tcgcggaggc
     1681 tatggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     1741 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     1801 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     1861 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     1921 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     1981 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     2041 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     2101 atcgccacga ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     2161 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     2221 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     2281 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     2341 ggcaaagaaa tagGCTTctc tagctagagt cgatcgacaa gctcgagttt ctccataata
     2401 atgtgtgagt agttcccaga taagggaatt agggttccta tagggtttcg ctcatgtgtt
     2461 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     2521 ttctaattcc taaaaCcaaa atccagtact aaaatccaga tcgctagcaa ggagTCTGAG
     2581 CAGGACAACT CGCGTAGTGA GAGTTACATG TTCGTTGGGT TCTTCCGACA CGGACCTGAG
     2641 TTGGCCAACG TCCCACCTGA GGTCTGTGCC CCGGTGATGA GAAGTGTGCA TCTCGTTCTT
     2701 GCAGCTCGTC AGTACTTTCA GAATCATGGC GTGCATGGTA GAATGACCCT TATAACGGAC
     2761 TTCGACATGG CAATcgctat acaggagTAT TCATTCAAAA GAGATTTTTA AAGATCAATT
     2821 TTTCTTTCTC ATAAGTCTAA TACGTCATGA TAATAACTTT AAAAAAAATT GAACTCGTTA
     2881 AAAAATTTGA TCAAATTTTA ATTTATTATT AGATCATATA TTACATATCA GTTTCATAAT
     2941 GTTATACTAA GATCTTTCAT ATATATTTTA ACCCACGAGA CAAAATATGT TTATATCTTT
     3001 ATTTATTTAA ATATTTTTTA GCTCAAAACA TCGCAAACTC AGCTATCATA ATGCTTTTTA
     3061 ATGAAAAAAA CAAATTTTCC TATCATACTC TCATTGTTTA TAATTTTTTA GAAAAAAAAC
     3121 TTGAAGATAA AATAACAACA ATAATAGTTT CAAAAGCACA TTTTTGTTGT AAATTTCAAG
     3181 AAAAATACAA TCATATTACT GATTGACTAC AAAAATTTGT AAATTAATCC AGGCTACATG
     3241 TTTAAGATGT GTCAAAACTG CATCATCTAC ATCTCATTTT AATTTTTCTA AACTACATAC
     3301 ATAGTATCAA TAACAATTGA ACAATGCTAG TTAATGTGTT ATACTCATTA GCTTGTAATA
     3361 TATCTTCAAC TTGATGAATC GGATTGATCA TAAAATTTCA CAAACTTATG TGCTGATTAA
     3421 TAAAAAATAT AATCAGATTT TTCATGAATA ATTAAGATAA ATAACACATG CAAGTCTCAA
     3481 TTAGAATTTA ACAATCTGAC TATAAAAAGT CGGTTTATCT TGATTTTCAC AAAAATTTAA
     3541 CATAGTTTCA AGGTGAATGG GCTTTTATTT TAATCCAATC AACTATTTGA CACATACATC
     3601 TTGCAAATTT ATTTATTTTT TATGGAAAAA AACATGATTT ATGTTTTTCT TTGAGAAAGT
     3661 ATATTGTGGA TTGTCAAAAA AAACCTATGG AAGATTTATA TGTAAGAGTT AAAAATTTAT
     3721 TTTCAAATAT GTCAACAATC CACAATATCG AAGAAAATTT TCAAAAAATC ATCACATATG
     3781 ATGTAAATAT AATATGAATA TGTGCAATCT TATTTCATTT TACTCTGAAA ACTTAATTTC
     3841 TTTTTATAGA GCATTCTGTA CTTACTGGTG TCAAATATAC AGATAAAATG CAGTCCATAA
     3901 CGTATCACTC TATAAGCAAA ATCATAAACT TCGTGATCTC TCACAGTTCA AACGAGAACT
     3961 ATAGAAACCC TTCAACTAGC AAAAAACTCA CAACTTTCTA TTCCAATATC TTTAATTGTC
     4021 TGACGATTTA CAAATAGAAA CGAAAACCAG ACACACACAC ACACGCAAAC TACAAAGTAA
     4081 AAACACAACC ACCTGACATT AGACAGTGAG ATTATTAAAC ATCCTAAACT AAAAAACAAA
     4141 CAAATTAATT CTCTATGTAC AAGTCCCCAT CGTGATCAAT TTTTGCATCC CAATATAGTG
     4201 GCGGTTAGTA TAAGAGGACC TTCGTCTATT CACTCTCCAT GTTTGTCAAA CTATAGTACA
     4261 GTACATATAT GTATGGGAAC AAATTCCGAA CACTCTAGTA CACTCCTCAA CGCAAGTTCT
     4321 ATCTCATATC TCTGCAAGAG CTTATTCCAA GAAACTTTCT TGTGCTTCTA TGCATGCCCA
     4381 GGTACCTTAA GTCGCGCGCA TAGTCACTTC ACAGCCTTTT CAGTTCTCCC TTTGATGGCT
     4441 CAGAGTGACG CAACTGCACA AAAATAAGAA AAAAGTCGAA GCGTCACGAG AGATGTATCC
     4501 GGCTAGTTTC CTTGGAGTTC GGATTGCTCT CTTTCTCTGT CTCCCCGAAC CATATGGAAT
     4561 TTATGAACTG CTGTTCACTA GTCAGTCACT TGTTTCAGAA CTTTTGAGTG GTCGATTTAT
     4621 CCACCTTCTT GTTTCTTCCT GGGTCCAGAA TCTGACTTCT TGAGACAACA AACAGTGCAC
     4681 AGTGGATCGA AGAATGACCA CAGTTGAGAC ACGTGGCGCT GGCCTCATTT ATTGCACCAT
     4741 ACGTTTAGTG AAGAACTGAA TAATGGTGGA TATAAAGCAG AGTTGCACCC AGCCTCTCGA
     4801 TAGTCTTTGT GAGAGAATGT tttagagcta gaaatagcaa gttaaaataa ggctagtccg
     4861 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tCTAGACCCA GCTTTCTTGT
     4921 ACAAAGTTGG Ccgctacaga ggagcaaatg agtcacacac attgttgaga gacatatcaa
     4981 aataactcaa ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat
     5041 atttgtatct caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa
     5101 ctctgtcttc gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt
     5161 tgctaaagat cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc
     5221 cgtttcacaa tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat
     5281 aaattgctta attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac
     5341 ccgcatcttc tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa
     5401 cttcgttctc caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt
     5461 ctaggttttc ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag
     5521 aagaaagcaa cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta
     5581 gtccaccgtt tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg
     5641 gccacctctc tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac
     5701 tcgaaaaacc ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc
     5761 ttattttgga atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga
     5821 cggaggagtg tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga
     5881 aggggcatgg acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc
     5941 gtcgtcggcc tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg
     6001 gccgccgacc ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc
     6061 tcgttattat aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc
     6121 gctaatcatt ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc
     6181 aaagctcgct gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt
     6241 tcctttcact tctttctctc ttgcgtggag agcatcgaca tcgaggagct gcTACTtgtc
     6301 ttctggtatg cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga
     6361 ttgtctagac ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga
     6421 gtgatttttt aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg
     6481 gtaaggtgtt cagttcggtc Actggtgcgg aggtgcctcg taagtttcct gcatgataga
     6541 tttgtctatc cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg
     6601 acgtcggtgc tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc
     6661 tgcagaaagg ttgtcaccaa tggataagaa gtactctatc ggactcgata tcggaactaa
     6721 ctctgtggga tgggctgtga tcaccgatga gtacaaggtg ccatctaaga agttcaaggt
     6781 tctcggaaac accgataggc actctatcaa gaaaaacctt atcggtgctc tcctcttcga
     6841 ttctggtgaa actgctgagg ctaccagact caagagaacc gctagaagaa ggtacaccag
     6901 aagaaagaac aggatctgct acctccaaga gatcttctct aacgagatgg ctaaagtgga
     6961 tgattcattc ttccacaggc tcgaagagtc attcctcgtg gaagaagata agaagcacga
     7021 gaggcaccct atcttcggaa acatcgttga tgaggtggca taccacgaga agtaccctac
     7081 tatctaccac ctcagaaaga agctcgttga ttctactgat aaggctgatc tcaggctcat
     7141 ctacctcgct ctcgctcaca tgatcaagtt cagaggacac ttcctcatcg agggtgatct
     7201 caaccctgat aactctgatg tggataagtt gttcatccag ctcgtgcaga cctacaacca
     7261 gcttttcgaa gagaacccta tcaacgcttc aggtgtggat gctaaggcta tcctctctgc
     7321 taggctctct aagtcaagaa ggcttgagaa cctcattgct cagctccctg gtgagaagaa
     7381 gaacggactt ttcggaaact tgatcgctct ctctctcgga ctcaccccta acttcaagtc
     7441 taacttcgat ctcgctgagg atgcaaagct ccagctctca aaggatacct acgatgatga
     7501 tctcgataac ctcctcgctc agatcggaga tcagtacgct gatttgttcc tcgctgctaa
     7561 gaacctctct gatgctatcc tcctcagtga tatcctcaga gtgaacaccg agatcaccaa
     7621 ggctccactc tcagcttcta tgatcaagag atacgatgag caccaccagg atctcacact
     7681 tctcaaggct cttgttagac agcagctccc agagaagtac aaagagattt tcttcgatca
     7741 gtctaagaac ggatacgctg gttacatcga tggtggtgca tctcaagaag agttctacaa
     7801 gttcatcaag cctatcctcg agaagatgga tggaaccgag gaactcctcg tgaagctcaa
     7861 tagagaggat cttctcagaa agcagaggac cttcgataac ggatctatcc ctcatcagat
     7921 ccacctcgga gagttgcacg ctatccttag aaggcaagag gatttctacc cattcctcaa
     7981 ggataacagg gaaaagattg agaagattct caccttcaga atcccttact acgtgggacc
     8041 tctcgctaga ggaaactcaa gattcgcttg gatgaccaga aagtctgagg aaaccatcac
     8101 cccttggaac ttcgaagagg tggtggataa gggtgctagt gctcagtctt tcatcgagag
     8161 gatgaccaac ttcgataaga accttccaaa cgagaaggtg ctccctaagc actctttgct
     8221 ctacgagtac ttcaccgtgt acaacgagtt gaccaaggtt aagtacgtga ccgagggaat
     8281 gaggaagcct gcttttttgt caggtgagca aaagaaggct atcgttgatc tcttgttcaa
     8341 gaccaacaga aaggtgaccg tgaagcagct caaagaggat tacttcaaga aaatcgagtg
     8401 cttcgattca gttgagattt ctggtgttga ggataggttc aacgcatctc tcggaaccta
     8461 ccacgatctc ctcaagatca ttaaggataa ggatttcttg gataacgagg aaaacgagga
     8521 tatcttggag gatatcgttc ttaccctcac cctctttgaa gatagagaga tgattgaaga
     8581 aaggctcaag acctacgctc atctcttcga tgataaggtg atgaagcagt tgaagagaag
     8641 aagatacact ggttggggaa ggctctcaag aaagctcatt aacggaatca gggataagca
     8701 gtctggaaag acaatccttg atttcctcaa gtctgatgga ttcgctaaca gaaacttcat
     8761 gcagctcatc cacgatgatt ctctcacctt taaagaggat atccagaagg ctcaggtttc
     8821 aggacagggt gatagtctcc atgagcatat cgctaacctc gctggatctc ctgcaatcaa
     8881 gaagggaatc ctccagactg tgaaggttgt ggatgagttg gtgaaggtga tgggaaggca
     8941 taagcctgag aacatcgtga tcgaaatggc tagagagaac cagaccactc agaagggaca
     9001 gaagaactct agggaaagga tgaagaggat cgaggaaggt atcaaagagc ttggatctca
     9061 gatcctcaaa gagcaccctg ttgagaacac tcagctccag aatgagaagc tctacctcta
     9121 ctacctccag aacggaaggg atatgtatgt ggatcaagag ttggatatca acaggctctc
     9181 tgattacgat gttgatcata tcgtgccaca gtcattcttg aaggatgatt ctatcgataa
     9241 caaggtgctc accaggtctg ataagaacag gggtaagagt gataacgtgc caagtgaaga
     9301 ggttgtgaag aaaatgaaga actattggag gcagctcctc aacgctaagc tcatcactca
     9361 gagaaagttc gataacttga ctaaggctga gaggggagga ctctctgaat tggataaggc
     9421 aggattcatc aagaggcagc ttgtggaaac caggcagatc actaagcacg ttgcacagat
     9481 cctcgattct aggatgaaca ccaagtacga tgagaacgat aagttgatca gggaagtgaa
     9541 ggttatcacc ctcaagtcaa agctcgtgtc tgatttcaga aaggatttcc aattctacaa
     9601 ggtgagggaa atcaacaact accaccacgc tcacgatgct taccttaacg ctgttgttgg
     9661 aaccgctctc atcaagaagt atcctaagct cgagtcagag ttcgtgtacg gtgattacaa
     9721 ggtgtacgat gtgaggaaga tgatcgctaa gtctgagcaa gagatcggaa aggctaccgc
     9781 taagtatttc ttctactcta acatcatgaa tttcttcaag accgagatta ccctcgctaa
     9841 cggtgagatc agaaagaggc cactcatcga gacaaacggt gaaacaggtg agatcgtgtg
     9901 ggataaggga agggatttcg ctaccgttag aaaggtgctc tctatgccac aggtgaacat
     9961 cgttaagaaa accgaggtgc agaccggtgg attctctaaa gagtctatcc tccctaagag
    10021 gaactctgat aagctcattg ctaggaagaa ggattgggac cctaagaaat acggtggttt
    10081 cgattctcct accgtggctt actctgttct cgttgtggct aaggttgaga agggaaagag
    10141 taagaagctc aagtctgtta aggaacttct cggaatcact atcatggaaa ggtcatcttt
    10201 cgagaagaac ccaatcgatt tcctcgaggc taagggatac aaagaggtta agaaggatct
    10261 catcatcaag ctcccaaagt actcactctt cgaactcgag aacggtagaa agaggatgct
    10321 cgcttctgct ggtgagcttc aaaagggaaa cgagcttgct ctcccatcta agtacgttaa
    10381 ctttctttac ctcgcttctc actacgagaa gttgaaggga tctccagaag ataacgagca
    10441 gaagcaactt ttcgttgagc agcacaagca ctacttggat gagatcatcg agcagatctc
    10501 tgagttctct aaaagggtga tcctcgctga tgcaaacctc gataaggtgt tgtctgctta
    10561 caacaagcac agagataagc ctatcaggga acaggcagag aacatcatcc atctcttcac
    10621 ccttaccaac ctcggtgctc ctgctgcttt caagtacttc gatacaacca tcgataggaa
    10681 gagatacacc tctaccaaag aagtgctcga tgctaccctc atccatcagt ctatcactgg
    10741 actctacgag actaggatcg atctctcaca gctcggtggt gattcaaggg ctgatcctaa
    10801 gaagaagagg aaggtttgag cttgagcgat cgttcaaaca tttggcaata aagtttctta
    10861 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
    10921 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
    10981 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
    11041 gataaattat cgcgcgcggt gtcatctatg ttactagatc GATCCGTATC GATAGCctct
    11101 agctagagtc gatcgacaag ctcgagtttc tccataataa tgtgtgagta gttcccagat
    11161 aagggaatta gggttcctat agggtttcgc tcatgtgttg agcatataag aaacccttag
    11221 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaTcaaaa
    11281 tccagtacta aaatccagat CGCTaggtat acttgagacc aGATCCtgac aggatatatt
    11341 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt
    11401 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
    11461 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
    11521 tgcagccgtc atctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
    11581 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
    11641 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
    11701 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
    11761 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
    11821 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
    11881 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
    11941 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
    12001 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
    12061 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca
    12121 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
    12181 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
    12241 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
    12301 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
    12361 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
    12421 ggccgggtac gtgttcgagc cgcccgcgca cctctcaacc gtgcggctgc atgaaatcct
    12481 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
    12541 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
    12601 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
    12661 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
    12721 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
    12781 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
    12841 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
    12901 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
    12961 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
    13021 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
    13081 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
    13141 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
    13201 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
    13261 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
    13321 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
    13381 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
    13441 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
    13501 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
    13561 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
    13621 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg cctgccggcc ctgcaatggc
    13681 actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac
    13741 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
    13801 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
    13861 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
    13921 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
    13981 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
    14041 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
    14101 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
    14161 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
    14221 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
    14281 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
    14341 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
    14401 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
    14461 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
    14521 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
    14581 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
    14641 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
    14701 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
    14761 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
    14821 gggaaaaagg tcgaaaagga ctctttcctg tggatagcac gtacattggg aacccaaagc
    14881 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
    14941 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
    15001 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
    15061 cagccgaagt gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
    15121 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
    15181 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
    15241 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
    15301 gtgacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
    15361 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
    15421 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    15481 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcatccgctt
    15541 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    15601 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    15661 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    15721 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    15781 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    15841 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    15901 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    15961 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    16021 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    16081 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    16141 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    16201 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    16261 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    16321 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt
    16381 ctaggtgatt atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct
    16441 tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta
    16501 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
    16561 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
    16621 actacatttc gctcatcacc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
    16681 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
    16741 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
    16801 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
    16861 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
    16921 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
    16981 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
    17041 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
    17101 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
    17161 gcgatcaccg cttccctcat aatgtttaac tttgttttag ggcgactgcc ctgctgcgta
    17221 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
    17281 gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc
    17341 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
    17401 cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgccttc
    17461 atccgtttcc acggtgtgcg tcacccggca accttgggta gcagcgaagt cgaggcattt
    17521 ctgtcctggc tgg
//