LOCUS       Exported                9129 bp ds-DNA     circular UNA 31-MAR-2016
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 4, 2020 from SnapGene 4.3.8.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    5453..5545
                     /label=Strep ii tag
                     /label=nonstandard type: tag
     primer_bind     complement(5522..5545)
                     /label=Strep RV (7002)
     CDS             5546..5860
                     /codon_start=1
                     /label=Sumo
                     /note="/cds_type=ORF"
                     /translation="TGSLQDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKT
                     TPLRRLMEAFAKRQGKEMDSLTFLYDGIEIQADQTPEDLDMEDNDIIEAHREQIGGIPT
                     T"
     site            5861..5884
                     /label=TEV cleavage site
                     /site_type="cleavage site"
     CDS             5885..8464
                     /codon_start=1
                     /label=hAgo2
                     /note="/cds_type=ORF"
                     /translation="MYSGAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFF
                     EMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAM
                     PLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALD
                     VVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSAT
                     AFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRV
                     CNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLP
                     LEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGI
                     MVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAP
                     QRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQL
                     VVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNN
                     ILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHR
                     QEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKL
                     EKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSH
                     AGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFR
                     ARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cagctttgtt tgtactatca acaggttgaa ctgctgatca
     4021 acagatcctc tacgcggccg cggtaccata acttcgtata gcatacatta tacgaagtta
     4081 tctggtttaa acgtacccgt agtggctatg gcagggcttg ccgccccgac gttggctgcg
     4141 agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga aacgcgggcg
     4201 tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc
     4261 ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt
     4321 catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc tcccccatct
     4381 cccggtaccg catgctccga ttcgatccta cttgtacagc tcgtccatgc cgagagtgat
     4441 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
     4501 gctcagggcg gactggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
     4561 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
     4621 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt
     4681 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
     4741 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
     4801 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
     4861 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg cgaagcactg
     4921 caggccgtag ccgaaggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
     4981 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
     5041 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
     5101 gaacagctcc tcgcccttgc tcaccatcat ggtttcggac cgcccgggtg atcaagtctt
     5161 cgtcgagtga ttgtaaataa aatgtaattt acagtatagt attttaatta atatacaaat
     5221 gatttgataa taattcttat ttaactataa tatattgtgt tgggttgaat taaaggtccg
     5281 tatactagta tcgattcgcg acctactccg gaatattaat agatcatgga gataattaaa
     5341 atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa
     5401 aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc ggatgtctgc
     5461 ttggtcacac cctcaattcg agaaaggcgg cggtagtggc ggcggatctg gcggtagtgc
     5521 ctggagccat ccgcagtttg aaaagaccgg ttccctgcag gactcagaag tcaatcaaga
     5581 agctaagcca gaggtcaagc cagaagtcaa gcctgagact cacatcaatt taaaggtgtc
     5641 cgatggatct tcagagatct tcttcaagat caaaaagacc actcctttaa gaaggctgat
     5701 ggaagcgttc gctaaaagac agggtaagga aatggactcc ttaacattct tgtacgacgg
     5761 tattgaaatt caagctgatc agacccctga agatttggac atggaggata acgatattat
     5821 tgaggctcac agagaacaga ttggtggaat cccaacgacc gaaaacctgt attttcaggg
     5881 cgccatgtac tcgggagccg gccccgcact tgcacctcct gcgccgccgc cccccatcca
     5941 aggatatgcc ttcaagcctc cacctagacc cgactttggg acctccggga gaacaatcaa
     6001 attacaggcc aatttcttcg aaatggacat ccccaaaatt gacatctatc attatgaatt
     6061 ggatatcaag ccagagaagt gcccgaggag agttaacagg gaaatcgtgg aacacatggt
     6121 ccagcacttt aaaacacaga tctttgggga tcggaagccc gtgtttgacg gcaggaagaa
     6181 tctatacaca gccatgcccc ttccgattgg gagggacaag gtggagctgg aggtcacgct
     6241 gccaggagaa ggcaaggatc gcatcttcaa ggtgtccatc aagtgggtgt cctgcgtgag
     6301 cttgcaggcg ttacacgatg cactttcagg gcggctgcct agcgtccctt ttgagacgat
     6361 ccaggccctg gacgtggtca tgaggcactt gccatccatg aggtacaccc ccgtgggccg
     6421 ctccttcttc accgcgtccg aaggctgctc taaccctctt ggcgggggcc gagaagtgtg
     6481 gtttggcttc catcagtccg tccggccttc tctctggaaa atgatgctga atattgatgt
     6541 gtcagcaaca gcgttttaca aggcacagcc agtaatcgag tttgtttgtg aagttttgga
     6601 ttttaaaagt attgaagaac aacaaaaacc tctgacagat tcccaaaggg taaagtttac
     6661 caaagaaatt aaaggtctaa aggtggagat aacgcactgt gggcagatga agaggaagta
     6721 ccgcgtctgc aatgtgaccc ggcggcccgc cagtcaccaa acattcccgc tgcagcagga
     6781 gagcgggcag acggtggagt gcacggtggc ccagtatttc aaggacaggc acaagcttgt
     6841 tctgcgctac ccccacctcc catgtttaca agtcggacag gagcagaaac acacctacct
     6901 tcccctggag gtctgtaaca ttgtggcagg acaaagatgt attaaaaaat taacggacaa
     6961 tcagacctca accatgatca gagcaactgc taggtcggcg cccgatcggc aagaagagat
     7021 tagcaaattg atgcgaagtg caagtttcaa cacagatcca tacgtccgtg aatttggaat
     7081 catggtcaaa gatgagatga cagacgtgac tgggcgggtg ctgcagccgc cctccatcct
     7141 ctacgggggc aggaataaag ctattgcgac ccctgtccag ggcgtctggg acatgcggaa
     7201 caagcagttc cacacgggca tcgagatcaa ggtgtgggcc attgcgtgct tcgcccccca
     7261 gcgccagtgc acggaagtcc atctgaagtc cttcacagag cagctcagaa agatctcgag
     7321 agacgctggc atgcccatcc agggccagcc gtgcttctgc aaatacgcgc agggggcgga
     7381 cagcgtggag cccatgttcc ggcacctgaa gaacacgtat gcgggcctgc agctggtggt
     7441 ggtcatcctg cccggcaaga cgcccgtgta cgccgaggtc aagcgcgtgg gagacacggt
     7501 gctggggatg gccacgcagt gcgtgcagat gaagaacgtg cagaggacca cgccacagac
     7561 cctgtccaac ctctgcctga agatcaacgt caagctggga ggcgtgaaca acatcctgct
     7621 gccccagggc aggccgccgg tgttccagca gcccgtcatc tttctgggag cagacgtcac
     7681 tcaccccccc gccggggatg ggaagaagcc ctccattgcc gccgtggtgg gcagcatgga
     7741 cgcccacccc aatcgctact gcgccaccgt gcgcgtgcag cagcaccggc aggagatcat
     7801 acaagacctg gccgccatgg tccgcgagct cctcatccag ttctacaagt ccacgcgctt
     7861 caagcccacc cgcatcatct tctaccgcga cggtgtctct gaaggccagt tccagcaggt
     7921 tctccaccac gagttgctgg ccatccgtga ggcctgtatc aagctagaaa aagactacca
     7981 gcccgggatc accttcatcg tggtgcagaa gaggcaccac acccggctct tctgcactga
     8041 caagaacgag cgggttggga aaagtggaaa cattccagca ggcacgactg tggacacgaa
     8101 aatcacccac cccaccgagt tcgacttcta cctgtgtagt cacgctggca tccaggggac
     8161 aagcaggcct tcgcactatc acgtcctctg ggacgacaat cgtttctcct ctgatgagct
     8221 gcagatccta acctaccagc tgtgtcacac ctacgtgcgc tgcacacgct ccgtgtccat
     8281 cccagcgcca gcatactacg ctcacctggt ggccttccgg gccaggtacc acctggtgga
     8341 taaggaacat gacagtgctg aaggaagcca tacctctggg cagagtaacg ggcgagacca
     8401 ccaagcactg gccaaggcgg tccaggttca ccaagacact ctgcgcacca tgtactttgc
     8461 ttgataggat cgaatcggac tttcgaatct agagcctgca gtctcgacaa gcttgtcgag
     8521 aagtactaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     8581 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     8641 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8701 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8761 catgtctgga tctgatcact gcttgagcct agaagatccg gctgctaaca aagcccgaaa
     8821 ggaagctgag ttggctgctg ccaccgctga gcaataacta tcataacccc taggagatcc
     8881 gaaccagata agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc
     8941 ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa
     9001 actccatttc cacccctccc agttcccaac tattttgtcc gcccacagcg gggcattttt
     9061 cttcctgtta tgtttttaat caaacatcct gccaactcca tgtgacaaac cgtcatcttc
     9121 ggctacttt
//
