LOCUS       ABE8.17-m               8166 bp    DNA     circular UNA 11-NOV-2019
DEFINITION  .
ACCESSION   urn.local...6s-823ng4u
VERSION     urn.local...6s-823ng4u
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..169
                     /created_by="User"
                     /label="CAG enhancer"
     misc_feature    126
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     promoter        456..525
                     /created_by="User"
                     /label="CMV promoter"
     promoter        546..564
                     /created_by="User"
                     /label="T7 protomet"
     CDS             <578..1078
                     /created_by="User"
                     /label="ecTadA_mmal codon optimize"
     misc_feature    644..646
                     /created_by="ngaudelli"
                     /label="W23R"
     misc_feature    683..685
                     /created_by="User"
                     /label="H36L"
     misc_feature    <719..721
                     /created_by="ngaudelli"
                     /modified_by="ngaudelli"
                     /label="P48A"
     misc_feature    728..730
                     /created_by="User"
                     /label="R51L"
     misc_feature    821..823
                     /created_by="nicolegaudelli"
                     /label="V82S"
     misc_feature    827..829
                     /created_by="User"
                     /modified_by="User"
                     /label="L84F"
     misc_feature    893..895
                     /created_by="User"
                     /label="A106V"
     misc_feature    899..901
                     /created_by="User"
                     /label="D108N"
     misc_feature    944..946
                     /created_by="User"
                     /modified_by="User"
                     /label="H123Y"
     misc_feature    1013..1015
                     /created_by="User"
                     /label="S146C"
     misc_feature    1016..1018
                     /created_by="User"
                     /label="D147Y"
     misc_feature    1031..1033
                     /created_by="ngaudelli"
                     /label="R152P"
     misc_feature    1037..1039
                     /created_by="nicolegaudelli"
                     /label="Q154R"
     misc_feature    1040..1042
                     /created_by="User"
                     /label="E155V"
     misc_feature    1043..1045
                     /created_by="User"
                     /modified_by="User"
                     /label="I156F"
     misc_feature    1046..1048
                     /created_by="User"
                     /label="K157N"
     CDS             1103..1150
                     /created_by="User"
                     /label="x ten "
     CDS             1175..5275
                     /note="hSpCas9"
                     /modified_by="jonlevy"
                     /label="fz hSpCas9"
     misc_feature    1465..1475
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /label="PflMI"
                     /note="Geneious type: restriction site"
     misc_feature    3646..3656
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /label="PflMI"
                     /note="Geneious type: restriction site"
     Primer          5288..>5320
                     /created_by="hrees"
                     /label="BPNLS"
     CDS             5321..5344
                     /created_by="User"
                     /modified_by="User"
                     /label="SV40 NLS"
     terminator      complement(5393..5620)
                     /created_by="User"
                     /label="bgH terminator"
     promoter        complement(5740..5769)
                     /created_by="User"
                     /label="lac promoter"
     rep_origin      6078..6697
                     /created_by="User"
                     /modified_by="User"
                     /label="pBR322"
     misc_feature    6289
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    6534
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     CDS             complement(6852..7712)
                     /created_by="User"
                     /label="beta-lactamase"
     promoter        complement(7754..7782)
                     /created_by="User"
                     /modified_by="User"
                     /label="pAmpR"
     promoter        8088..498
                     /created_by="User"
                     /modified_by="User"
                     /label="pCMV immearly protomter"
ORIGIN      
        1 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
       61 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      121 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      181 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      241 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      301 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      361 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      421 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      481 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg
      541 gccgctaata cgactcacta tagggagagc cgccaccatg tccgaagtcg agttttccca
      601 tgagtactgg atgagacacg cattgactct cgcaaagagg gctcgagatg aacgcgaggt
      661 gcccgtgggg gcagtactcg tgctcaacaa tcgcgtaatc ggcgaaggtt ggaatagggc
      721 aatcggactc cacgacccca ctgcacatgc ggaaatcatg gcccttcgac agggagggct
      781 tgtgatgcag aattatcgac ttatcgatgc gacgctgtac tcgacgtttg aaccttgcgt
      841 aatgtgcgcg ggagctatga ttcactcccg cattggacga gttgtattcg gtgttcgcaa
      901 cgccaagacg ggtgccgcag gttcactgat ggacgtgctg cattacccag gcatgaacca
      961 ccgggtagaa atcacagaag gcatattggc ggacgaatgt gcggcgctgt tgtgttactt
     1021 ttttcgcatg cccaggcgtg tctttaacgc ccagaaaaaa gcacaatcct ctactgactc
     1081 tggtggttct tctggtggtt ctagcggcag cgagactccc gggacctcag agtccgccac
     1141 acccgaaagt tctggtggtt cttctggtgg ttctgacaag aagtacagca tcggcctggc
     1201 catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa
     1261 gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc
     1321 cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag
     1381 aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat
     1441 ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga
     1501 taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga
     1561 gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga
     1621 cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat
     1681 cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca
     1741 gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc
     1801 catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc
     1861 cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc
     1921 caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac
     1981 ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt
     2041 tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac
     2101 cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca
     2161 ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat
     2221 tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga
     2281 agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct
     2341 cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat
     2401 cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta
     2461 cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta
     2521 ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga
     2581 ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag
     2641 cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa
     2701 gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt
     2761 gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga
     2821 cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa
     2881 gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc
     2941 cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga
     3001 ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga
     3061 gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca
     3121 gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat
     3181 ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa
     3241 cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa
     3301 agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag
     3361 ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt
     3421 gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac
     3481 ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga
     3541 gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa
     3601 gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat
     3661 caaccggctg tccgactacg atgtggacca tatcgtgcct cagagctttc tgaaggacga
     3721 ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt
     3781 gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa
     3841 gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga
     3901 actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca
     3961 cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat
     4021 ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt
     4081 ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa
     4141 cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta
     4201 cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg
     4261 caaggctacc gccaagtact tcttctacag caacatcatg aactttttca agaccgagat
     4321 taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg
     4381 ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc
     4441 ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat
     4501 cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa
     4561 gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga
     4621 aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga
     4681 aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt
     4741 gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg
     4801 gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc
     4861 caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga
     4921 ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat
     4981 cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt
     5041 gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat
     5101 ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac
     5161 catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca
     5221 gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag gtgacgaggg
     5281 agctgataag cgcaccgccg atggttccga gttcgaaagc cccaagaaga agaggaaagt
     5341 ctaaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
     5401 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     5461 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     5521 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     5581 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     5641 agctggggct cgataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     5701 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     5761 aagtgtaaag cctagggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     5821 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     5881 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     5941 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6001 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6061 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6121 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6181 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6241 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6301 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6361 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6421 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6481 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     6541 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6601 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6661 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6721 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6781 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6841 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6901 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6961 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7021 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7081 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7141 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7201 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7261 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7321 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7381 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7441 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     7501 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     7561 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     7621 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     7681 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     7741 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7801 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     7861 gatcgatctc ccgatcccct agggtcgact ctcagtacaa tctgctctga tgccgcatag
     7921 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     7981 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     8041 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     8101 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     8161 cgcgtt
//
