LOCUS ABE8.20-d 8760 bp DNA circular UNA 11-NOV-2019 DEFINITION . ACCESSION urn.local...6s-823ng4u VERSION urn.local...6s-823ng4u KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 325..901 /created_by="User" /modified_by="User" /label="pCMV immearly protomter" misc_feature 404..572 /created_by="User" /label="CAG enhancer" misc_feature 529 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" promoter 859..928 /created_by="User" /label="CMV promoter" promoter 949..967 /created_by="User" /label="T7 protomet" CDS 981..1481 /created_by="User" /label="ecTadA_mmal codon optimize" gene 1482..1493 /created_by="User" /label="GGS" mat_peptide <1482..>1493 /created_by="User" /label="gene block" gene 1494..1505 /created_by="User" /label="GGS" mat_peptide <1494..>1505 /created_by="User" /label="gene block" CDS 1506..1553 /created_by="User" /label="x ten " gene 1554..1565 /created_by="User" /label="GGS" mat_peptide <1554..>1565 /created_by="User" /label="gene block" gene 1566..1577 /created_by="User" /label="GGS" mat_peptide <1566..>1577 /created_by="User" /label="gene block" CDS <1578..2075 /created_by="User" /codon_start=1 /modified_by="nicolegaudelli" /label="ecTadA_mmal codon optimize" misc_feature 1641..1643 /created_by="ngaudelli" /label="W23R" misc_feature 1680..1682 /created_by="User" /label="H36L" misc_feature <1716..1718 /created_by="ngaudelli" /modified_by="ngaudelli" /label="P48A" misc_feature 1725..1727 /created_by="User" /label="R51L" misc_feature 1800..1802 /created_by="nicolegaudelli" /label="I76Y" misc_feature 1818..1820 /created_by="nicolegaudelli" /label="V82S" misc_feature 1824..1826 /created_by="User" /modified_by="User" /label="L84F" misc_feature 1890..1892 /created_by="User" /label="A106V" misc_feature 1896..1898 /created_by="User" /label="D108N" misc_feature 1941..1943 /created_by="nicolegaudelli" /label="Y123H" misc_feature 2010..2012 /created_by="User" /label="S146C" misc_feature 2013..2015 /created_by="nicolegaudelli" /label="Y147R" misc_feature 2028..2030 /created_by="ngaudelli" /label="R152P" misc_feature 2034..>2036 /created_by="nicolegaudelli" /label="Q154R" misc_feature 2037..2039 /created_by="User" /label="E155V" misc_feature 2040..2042 /created_by="User" /modified_by="User" /label="I156F" misc_feature 2043..2045 /created_by="User" /label="K157N" CDS 2100..2147 /created_by="User" /label="x ten " CDS 2172..6272 /note="hSpCas9" /modified_by="jonlevy" /label="fz hSpCas9" misc_feature 2462..2472 /Recognition_pattern="CCANNNN^NTGG" /annotation_group="CCANNNNNTGG" /label="PflMI" /note="Geneious type: restriction site" misc_feature 4643..4653 /Recognition_pattern="CCANNNN^NTGG" /annotation_group="CCANNNNNTGG" /label="PflMI" /note="Geneious type: restriction site" Primer 6285..>6317 /created_by="hrees" /label="BPNLS" CDS 6318..6341 /created_by="User" /modified_by="User" /label="SV40 NLS" terminator complement(6390..6617) /created_by="User" /label="bgH terminator" promoter complement(6737..6766) /created_by="User" /label="lac promoter" rep_origin 7075..7694 /created_by="User" /modified_by="User" /label="pBR322" misc_feature 7286 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature 7531 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Replacement" CDS complement(7849..8709) /created_by="User" /label="beta-lactamase" promoter complement(8751..19) /created_by="User" /modified_by="User" /label="pAmpR" ORIGIN 1 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 61 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat cgatctcccg atcccctagg 121 gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc 181 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg 241 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga 301 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat 361 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 421 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 481 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 541 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 601 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 661 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca 721 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat 781 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa 841 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 901 cagagctggt ttagtgaacc gtcagatccg ctagagatcc gcggccgcta atacgactca 961 ctatagggag agccgccacc atgtccgaag tcgagttttc ccatgagtac tggatgagac 1021 acgcattgac tctcgcaaag agggcttggg atgaacgcga ggtgcccgtg ggggcagtac 1081 tcgtgcataa caatcgcgta atcggcgaag gttggaatag gccgatcgga cgccacgacc 1141 ccactgcaca tgcggaaatc atggcccttc gacagggagg gcttgtgatg cagaattatc 1201 gacttatcga tgcgacgctg tacgtcacgc ttgaaccttg cgtaatgtgc gcgggagcta 1261 tgattcactc ccgcattgga cgagttgtat tcggtgcccg cgacgccaag acgggtgccg 1321 caggttcact gatggacgtg ctgcatcacc caggcatgaa ccaccgggta gaaatcacag 1381 aaggcatatt ggcggacgaa tgtgcggcgc tgttgtccga cttttttcgc atgcggaggc 1441 aggagatcaa ggcccagaaa aaagcacaat cctctactga ctctggtggt tcttctggtg 1501 gttctagcgg cagcgagact cccgggacct cagagtccgc cacacccgaa agttctggtg 1561 gttcttctgg tggttcttcc gaagtcgagt tttcccatga gtactggatg agacacgcat 1621 tgactctcgc aaagagggct cgagatgaac gcgaggtgcc cgtgggggca gtactcgtgc 1681 tcaacaatcg cgtaatcggc gaaggttgga atagggcaat cggactccac gaccccactg 1741 cacatgcgga aatcatggcc cttcgacagg gagggcttgt gatgcagaat tatcgacttt 1801 atgatgcgac gctgtactcg acgtttgaac cttgcgtaat gtgcgcggga gctatgattc 1861 actcccgcat tggacgagtt gtattcggtg ttcgcaacgc caagacgggt gccgcaggtt 1921 cactgatgga cgtgctgcat catccaggca tgaaccaccg ggtagaaatc acagaaggca 1981 tattggcgga cgaatgtgcg gcgctgttgt gtcgtttttt tcgcatgccc aggcgggtct 2041 ttaacgccca gaaaaaagca caatcctcta ctgactctgg tggttcttct ggtggttcta 2101 gcggcagcga gactcccggg acctcagagt ccgccacacc cgaaagttct ggtggttctt 2161 ctggtggttc tgacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct 2221 gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca 2281 ccgaccggca cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa 2341 cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc 2401 ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct 2461 tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca 2521 tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc 2581 tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc 2641 tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca 2701 acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg 2761 aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca 2821 agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt 2881 tcggaaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc 2941 tggccgagga tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc 3001 tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg 3061 acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga 3121 gcgcctctat gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc 3181 tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg 3241 gctacgccgg ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc 3301 ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc 3361 tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag 3421 agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg 3481 aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg 3541 gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact 3601 tcgaggaagt ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact 3661 tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact 3721 tcaccgtgta taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg 3781 ccttcctgag cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga 3841 aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg 3901 tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc 3961 tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag 4021 atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa 4081 cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg 4141 gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga 4201 caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc 4261 acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg 4321 atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc 4381 tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga 4441 acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc 4501 gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag 4561 aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga 4621 atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg 4681 tggaccatat cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga 4741 ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga 4801 agatgaagaa ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg 4861 acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca 4921 agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc 4981 ggatgaacac taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc 5041 tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga 5101 tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga 5161 tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg 5221 tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct 5281 tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc 5341 ggaagcggcc tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc 5401 gggattttgc caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga 5461 ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata 5521 agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca 5581 ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga 5641 agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc 5701 ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc 5761 tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg 5821 gcgaactgca gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc 5881 tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt 5941 ttgtggaaca gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca 6001 agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc 6061 gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc 6121 tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca 6181 gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga 6241 cacggatcga cctgtctcag ctgggaggtg acgagggagc tgataagcgc accgccgatg 6301 gttccgagtt cgaaagcccc aagaagaaga ggaaagtcta accggtcatc atcaccatca 6361 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt 6421 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 6481 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg 6541 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga 6601 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga taccgtcgac 6661 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 6721 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct agggtgccta 6781 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 6841 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 6901 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6961 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 7021 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 7081 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 7141 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 7201 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 7261 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 7321 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 7381 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 7441 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 7501 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 7561 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 7621 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 7681 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 7741 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 7801 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 7861 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 7921 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 7981 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 8041 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 8101 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 8161 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 8221 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 8281 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 8341 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 8401 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 8461 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 8521 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 8581 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 8641 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 8701 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat //