LOCUS       Exported                5087 bp ds-DNA     circular SYN 01-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Nov 11, 2019 from SnapGene Viewer 4.0.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2250..2283
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2..25
                     /label=M13-F1
                     /label=PCR_primer
                     /note="M13-F1
                     ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    37..149
                     /label=attL5
                     /note="ApEinfo_fwdcolor=#c0c0c0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#c0c0c0
                     dnas_title=attL5
                     vntifkey=21"
     promoter        167..2129
                     /label=Ubi1 (maize ubiquitin promoter 1) promoter
                     /label=Ubi1 (maize ubiquitin promoter 1)
                     /note="Ubi1 (maize ubiquitin promoter 1)
                     ApEinfo_fwdcolor=#346ee0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#346ee0"
     misc_feature    2136..2141
                     /label=1st 6nt complementary
     misc_feature    2142..2178
                     /label=Hammerhead ribozyme
     misc_feature    2179..2293
                     /label=sgRNA3.8.4
     stem_loop       2250..2283
                     /label=MS2(own)
                     /note="guide RNA scaffold 2.0"
     misc_recomb     2294..2325
                     /label=Guide RNA cloning site
                     /note="Guide RNA cloning site
                     ApEinfo_fwdcolor=#8080ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#da6a3b"
     stem_loop       2326..2393
                     /label=HDV ribozyme
                     /note="HDV ribozyme
                     ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    2413..2512
                     /label=attL2
                     /note="ApEinfo_fwdcolor=#ff0080
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff0080
                     dnas_title=attL2
                     vntifkey=21"
     misc_feature    2542..2576
                     /label=extra seq
                     /note="extra seq
                     ApEinfo_fwdcolor=#7eff74
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#7eff74"
     primer_bind     complement(2584..2604)
                     /label=M13-rev
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green
                     dnas_title=M13-rev
                     vntifkey=28"
     misc_feature    2682..2815
                     /label=Spn promoter
                     /note="ApEinfo_fwdcolor=#2fec13
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#2fec13
                     dnas_title=Spn promoter
                     vntifkey=21"
     misc_feature    2716
                     /label=G to A mutation
     misc_feature    2816..3826
                     /label=SpnR
                     /note="ApEinfo_fwdcolor=#f70938
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#f70938
                     dnas_title=SpnR
                     vntifkey=21"
     misc_feature    3893..4566
                     /label=pUC origin
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green
                     dnas_title=pUC origin
                     vntifkey=21"
ORIGIN
        1 tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc aaataatgat
       61 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
      121 aatgccaact ttgtatacaa aagttgcccc atggcgttcc ctctagagag ataatgagca
      181 ttgcatgtct aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg
      241 cagtttatct atctttatac atatatttaa actttactct acgaataata taatctatag
      301 tactacaata atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa
      361 aggacaattg agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg
      421 ttctcctttt tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac
      481 atccatttag ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt
      541 tattctattt tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa
      601 taatttagat ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa
      661 gaaattaaaa aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta
      721 aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca
      781 agcgaagcag acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc
      841 tccaccgttg gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg
      901 tgagccggca cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt
      961 cctttcccac cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct
     1021 ccacaccctc tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc
     1081 ccccaaatcc acccgtcggc acctccgctt caaggtacgc cgctcgtcct cccccccccc
     1141 cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac
     1201 ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta
     1261 cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt
     1321 ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt
     1381 gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt
     1441 gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt
     1501 gggcggtcgt tctagatcgg agtagaatta attctgtttc aaactacctg gtggatttat
     1561 taattttgga tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg
     1621 atggaaatat cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac
     1681 agagatgctt tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc
     1741 gttctagatc ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac
     1801 tgtatgtgtg tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc
     1861 taggataggt atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca
     1921 gcatctattc atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt
     1981 ataattattt tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt
     2041 tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct
     2101 caccctgttg tttggtgtta cttctgcagg gatccttaga cctgatgagt ccgtgaggac
     2161 gaaacgagta agctcgtcgt ctaaaggaca gaatttttca acgggtgtgc caatggccac
     2221 tttccaggtg gcaaagcccg ttgaacttcg gccaacatga ggatcaccca tgtctgcagg
     2281 gccgaagtgg cacgagacgg agctcagtct gaccgcggcg tctctggccg gcatggtccc
     2341 agcctcctcg ctggcgccgg ctgggcaaca tgcttcggca tggcgaatgg gacggtaccc
     2401 ggccggaatt cgacccagct ttcttgtaca aagttggcat tataaaaaat aattgctcat
     2461 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
     2521 ctgatatccc ctatagtgag tcgtattaca tggtcatagc atcccctata gtgagtcgta
     2581 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2641 cattgcacaa gataaaaata tatcatcatg cctcctctgg accagccagg acagaaatgc
     2701 ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     2761 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     2821 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     2881 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     2941 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     3001 gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt
     3061 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     3121 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     3181 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     3241 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     3301 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     3361 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     3421 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     3481 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     3541 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     3601 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     3661 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     3721 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     3781 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
     3841 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     3901 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3961 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4021 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     4081 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4141 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4201 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4261 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag
     4321 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4381 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4441 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4501 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4561 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     4621 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     4681 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     4741 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac
     4801 gcgtaccgct cgccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
     4861 ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
     4921 cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
     4981 accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt
     5041 tgatgcctgg cagttcccta ctctcgcgtt aacgcttgca tggatgt
//
