LOCUS       Exported                5069 bp ds-DNA     circular SYN 26-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 11, 2019 from SnapGene Viewer 4.0.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..2178
                     /label=source
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     dnas_title=pYPQ141-ZmUbi-RZ-Lb"
     source          1..162
                     /label=source(1)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=source
                     ApEinfo_revcolor=pink
                     dnas_title=pYPQ104_correct_"
     source          2131..2135
                     /label=source(2)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=source
                     ApEinfo_revcolor=pink
                     dnas_title=RZ-AsCpf1-OsYsA-gR1"
     source          2276..5069
                     /label=source(4)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=source
                     ApEinfo_revcolor=pink
                     dnas_title=pYPQ141-ZmUbi-RZ-Lb"
     source          2376..2388
                     /label=source(3)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=source
                     ApEinfo_revcolor=pink
                     dnas_title=DA1-RZ-gR1"
     misc_feature    2..25
                     /label=M13-F1
                     /label=PCR_primer
                     /note="M13-F1
                     ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    37..149
                     /label=attL5
                     /note="ApEinfo_fwdcolor=#c0c0c0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#c0c0c0
                     dnas_title=attL5
                     vntifkey=21"
     promoter        167..2129
                     /label=Ubi1 (maize ubiquitin promoter 1) promoter
                     /label=Ubi1 (maize ubiquitin promoter 1)
                     /note="Ubi1 (maize ubiquitin promoter 1)
                     ApEinfo_fwdcolor=#346ee0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#346ee0"
     misc_feature    2136..2141
                     /label=6nt complementary
     stem_loop       2142..2178
                     /label=Hammerhead ribozyme
                     /note="Hammerhead ribozyme
                     ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    2179..2275
                     /label=BhCas12b sgRNA
     misc_recomb     2276..2307
                     /label=Guide RNA cloning site
                     /note="Guide RNA cloning site
                     ApEinfo_fwdcolor=#8080ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#da6a3b"
     stem_loop       2308..2375
                     /label=HDV ribozyme
                     /note="HDV ribozyme
                     ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    2395..2494
                     /label=attL2
                     /note="ApEinfo_fwdcolor=#ff0080
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff0080
                     dnas_title=attL2
                     vntifkey=21"
     misc_feature    2524..2558
                     /label=extra seq
                     /note="extra seq
                     ApEinfo_fwdcolor=#7eff74
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#7eff74"
     primer_bind     complement(2566..2586)
                     /label=M13-rev
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green
                     dnas_title=M13-rev
                     vntifkey=28"
     misc_feature    complement(2587..2609)
                     /label=M13-R1
     misc_feature    2664..2797
                     /label=Spn promoter
                     /note="ApEinfo_fwdcolor=#2fec13
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#2fec13
                     dnas_title=Spn promoter
                     vntifkey=21"
     misc_feature    2698
                     /label=G to A mutation
     misc_feature    2798..3808
                     /label=SpnR
                     /note="ApEinfo_fwdcolor=#f70938
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#f70938
                     dnas_title=SpnR
                     vntifkey=21"
     misc_feature    3875..4548
                     /label=pUC origin
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green
                     dnas_title=pUC origin
                     vntifkey=21"
ORIGIN
        1 tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc aaataatgat
       61 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
      121 aatgccaact ttgtatacaa aagttgcccc atggcgttcc ctctagagag ataatgagca
      181 ttgcatgtct aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg
      241 cagtttatct atctttatac atatatttaa actttactct acgaataata taatctatag
      301 tactacaata atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa
      361 aggacaattg agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg
      421 ttctcctttt tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac
      481 atccatttag ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt
      541 tattctattt tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa
      601 taatttagat ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa
      661 gaaattaaaa aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta
      721 aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca
      781 agcgaagcag acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc
      841 tccaccgttg gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg
      901 tgagccggca cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt
      961 cctttcccac cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct
     1021 ccacaccctc tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc
     1081 ccccaaatcc acccgtcggc acctccgctt caaggtacgc cgctcgtcct cccccccccc
     1141 cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac
     1201 ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta
     1261 cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt
     1321 ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt
     1381 gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt
     1441 gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt
     1501 gggcggtcgt tctagatcgg agtagaatta attctgtttc aaactacctg gtggatttat
     1561 taattttgga tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg
     1621 atggaaatat cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac
     1681 agagatgctt tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc
     1741 gttctagatc ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac
     1801 tgtatgtgtg tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc
     1861 taggataggt atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca
     1921 gcatctattc atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt
     1981 ataattattt tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt
     2041 tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct
     2101 caccctgttg tttggtgtta cttctgcagg gatcccagaa cctgatgagt ccgtgaggac
     2161 gaaacgagta agctcgtcgt tctgtctttt ggtcaggaca accgtctagc tataagtgct
     2221 gcagggtgtg agaaactcct attgctggac gatgtctctt acgaggcatt agcacgagac
     2281 ggagctcagt ctgaccgcgg cgtctctggc cggcatggtc ccagcctcct cgctggcgcc
     2341 ggctgggcaa catgcttcgg catggcgaat gggacggtac ccggccggaa ttcgacccag
     2401 ctttcttgta caaagttggc attataaaaa ataattgctc atcaatttgt tgcaacgaac
     2461 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     2521 agtcgtatta catggtcata gcatccccta tagtgagtcg tattacatgg tcatagctgt
     2581 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     2641 tatatcatca tgcctcctct ggaccagcca ggacagaaat gcctcgactt cgctgctgcc
     2701 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
     2761 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     2821 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     2881 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     2941 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     3001 aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     3061 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     3121 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     3181 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     3241 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     3301 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     3361 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     3421 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     3481 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     3541 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     3601 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     3661 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     3721 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     3781 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa
     3841 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3901 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3961 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4021 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4081 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4141 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4201 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4261 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg
     4321 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4381 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4441 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4501 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4561 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4621 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     4681 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     4741 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctcgccagga
     4801 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
     4861 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
     4921 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     4981 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     5041 tactctcgcg ttaacgcttg catggatgt
//
