LOCUS Exported 6107 bp ds-DNA circular SYN 15-NOV-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 6107) AUTHORS Stanford University TITLE Direct Submission JOURNAL Exported Friday, Nov 15, 2019 from SnapGene 5.0.0 https://www.snapgene.com FEATURES Location/Qualifiers source 1..6107 /organism="recombinant plasmid" /mol_type="other DNA" misc_feature 11..586 /label=cmv promoter enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 573..638 /label=iLID mch iTrkA bc fwd misc_feature 591..617 /label=MCS primer_bind 603..646 /label=iLID fwd primer_bind 603..638 /label=iLID fwd correct CDS 622..1068 /codon_start=1 /label=iLID /translation="GEFLATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEI LGRNCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKG DVQYFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF" CDS 1045..1065 /label=SsrA primer_bind complement(1069..1103) /label=iLID rev misc_feature 1105..1110 /label=Kozak CDS 1111..1818 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" misc_feature 1819..1827 /label=GSS CDS 1828..2877 /codon_start=1 /label=iTrkA /translation="KFGINRPAVLAPEDGLAMSLHFMTLGGSSLSPTEGKGSGLQGHIM ENPQYFSDTCVHHIKRQDIILKWELGEGAFGKVFLAECYNLLNDQDKMLVAVKALKETS ENARQDFHREAELLTMLQHQHIVRFFGVCTEGGPLLMVFEYMRHGDLNRFLRSHGPDAK LLAGGEDVAPGPLGLGQLLAVASQVAAGMVYLASLHFVHRDLATRNCLVGQGLVVKIGD FGMSRDIYSTDYYRVGGRTMLPIRWMPPESILYRKFSTESDVWSFGVVLWEIFTYGKQP WYQLSNTEAIECITQGRELERPRACPPDVYAIMRGCWQREPQQRLSMKDVHARLQALAQ APPSYLDVLG" primer_bind complement(2857..2877) /label=iLID mch iTrkA bc rev polyA_signal 2895..3016 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(3023..3478) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3505..3609 /gene="bla" /label=AmpR promoter promoter 3611..3968 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3819..3954 /label=SV40 ori /note="SV40 origin of replication" CDS 4003..4797 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 5029..5076 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5405..5993 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagat gggggagttt ctggcaacca cactggaacg gatcgagaaa 661 aatttcgtga ttactgatcc gagactgcct gacaacccaa tcatttttgc gagcgattcc 721 ttcctgcagc tgacagaata ttctcgggaa gagatcctgg ggcgcaattg ccgttttctg 781 cagggacccg agacagaccg tgccactgtt cggaaaatca gagatgctat tgacaaccag 841 actgaagtga ccgttcagct gatcaattat accaagagcg gcaagaagtt ctggaacgtg 901 ttccacctgc agccgatgcg cgattataag ggcgacgtcc agtacttcat tggcgtgcag 961 ctggatggca ccgaacgtct tcatggcgcc gctgagcgtg aggcggtctg cctgatcaaa 1021 aagacagcct ttcagattgc tgaggcagcg aacgacgaaa attactttgg aagcgggagt 1081 gggagccccc gggatccacc ggtcgccacc atggtgagca agggcgagga ggataacatg 1141 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac 1201 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag 1261 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc 1321 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg 1381 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg 1441 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc 1501 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc 1561 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg 1621 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag 1681 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac 1741 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc 1801 atggacgagc tgtacaaggg ttcttctaaa tttgggatca accgccctgc tgtgctggcg 1861 ccagaggatg ggctggccat gtccctacac ttcatgacac tgggtggcag ttctctttcc 1921 cctactgagg gcaaaggctc cggactccag ggccacatca tggagaaccc acagtacttc 1981 agtgatacct gtgtccacca tatcaagcgc caggacatca ttctcaagtg ggagctaggg 2041 gagggagcct ttggaaaggt ctttcttgct gagtgctaca accttctgaa tgatcaggac 2101 aagatgctgg tggctgtcaa ggcactgaag gagacatctg agaatgctcg tcaggacttc 2161 catcgtgagg cagagctgct caccatgcta cagcaccaac acatcgtacg cttctttgga 2221 gtctgcacgg agggtggccc attgctcatg gtcttcgagt acatgcgcca tggggacctc 2281 aaccgtttcc tccggtccca cggacctgat gcaaaactgc tggctggcgg cgaggatgtg 2341 gctcctggtc ctttgggcct tgggcagctt ctggctgtgg ctagccaggt ggctgctggt 2401 atggtgtacc tagccagcct gcactttgtg caccgggacc tggccacacg caactgtctg 2461 gtgggtcagg gactagtggt caagattgga gacttcggca tgagcaggga catctacagc 2521 acagactact accgtgtggg aggtcggacc atgctgccca tccgctggat gcctccagag 2581 agcatcctct accgcaagtt cagcaccgag agtgatgtgt ggagcttcgg ggtggtgctc 2641 tgggagatct tcacctatgg aaagcaaccc tggtaccagc tctccaacac tgaggcgatc 2701 gagtgcatca cgcagggccg ggagctggag cggccgcgcg cctgccctcc tgatgtctac 2761 gccatcatgc gcggctgctg gcagcgtgag ccgcaacagc gcctcagcat gaaggatgtg 2821 cacgcgcggc tgcaggcctt ggcacaggcg ccaccgagtt acctggacgt tctgggttag 2881 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 2941 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 3001 tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta 3061 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 3121 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca 3181 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 3241 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta 3301 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 3361 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg 3421 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca 3481 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 3541 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 3601 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa 3661 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 3721 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 3781 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 3841 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 3901 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 3961 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 4021 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 4081 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 4141 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg 4201 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 4261 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 4321 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 4381 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 4441 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 4501 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg 4561 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 4621 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 4681 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 4741 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 4801 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 4861 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 4921 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc 4981 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa 5041 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg 5101 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt 5161 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg 5221 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac 5281 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 5341 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 5401 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 5461 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 5521 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 5581 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 5641 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 5701 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 5761 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 5821 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 5881 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 5941 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 6001 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 6061 ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat //