LOCUS       pVimentin-miRFP703        6322 bp    DNA     circular      7-MAY-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
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COMMENT     VNTDBDATE|-12278346|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     misc_feature    61..568
                     /vntifkey="21"
                     /label=CMV
     misc_feature    4218..5012
                     /vntifkey="21"
                     /label=NeoR/KanR
     misc_feature    637..2034
                     /vntifkey="21"
                     /label=Vimentin
     misc_feature    2056..2985
                     /vntifkey="21"
                     /label=emiRFP703
BASE COUNT     1457 a      1737 c      1731 g      1397 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attcccatgt ccaccaggtc cgtgtcctcg 
      661 tcctcctacc gcaggatgtt cggcggcccg ggcaccgcga gccggccgag ctccagccgg 
      721 agctacgtga ctacgtccac ccgcacctac agcctgggca gcgcgctgcg ccccagcacc 
      781 agccgcagcc tctacgcctc gtccccgggc ggcgtgtatg ccacgcgctc ctctgccgtg 
      841 cgcctgcgga gcagcgtgcc cggggtgcgg ctcctgcagg actcggtgga cttctcgctg 
      901 gccgacgcca tcaacaccga gttcaagaac acccgcacca acgagaaggt ggagctgcag 
      961 gagctgaatg accgcttcgc caactacatc gacaaggtgc gcttcctgga gcagcagaat 
     1021 aagatcctgc tggccgagct cgagcagctc aagggccaag gcaagtcgcg cctgggggac 
     1081 ctctacgagg aggagatgcg ggagctgcgc cggcaggtgg accagctaac caacgacaaa 
     1141 gcccgcgtcg aggtggagcg cgacaacctg gccgaggaca tcatgcgcct ccgggagaaa 
     1201 ttgcaggagg agatgcttca gagagaggaa gccgaaaaca ccctgcaatc tttcagacag 
     1261 gatgttgaca atgcgtctct ggcacgtctt gaccttgaac gcaaagtgga atctttgcaa 
     1321 gaagagattg cctttttgaa gaaactccac gaagaggaaa tccaggagct gcaggctcag 
     1381 attcaggaac agcatgtcca aatcgatgtg gatgtttcca agcctgacct cacggctgcc 
     1441 ctgcgtgacg tacgtcagca atatgaaagt gtggctgcca agaacctgca ggaggcagaa 
     1501 gaatggtaca aatccaagtt tgctgacctc tctgaggctg ccaaccggaa caatgacgcc 
     1561 ctgcgccagg caaagcagga gtccactgag taccggagac aggtgcagtc cctcacctgt 
     1621 gaagtggatg cccttaaagg aaccaatgag tccctggaac gccagatgcg tgaaatggaa 
     1681 gagaactttg ccgttgaagc tgctaactac caagacacta ttggccgcct gcaggatgag 
     1741 attcagaata tgaaggagga aatggctcgt caccttcgtg aataccaaga cctgctcaat 
     1801 gttaagatgg cccttgacat tgagattgcc acctacagga agctgctgga aggcgaggag 
     1861 agcaggattt ctctgcctct tccaaacttt tcctccctga acctgaggga aactaatctg 
     1921 gattcactcc ctctggttga tacccactca aaaaggacac ttctgattaa gacggttgaa 
     1981 actagagatg gacaggttat caacgaaact tctcagcatc acgatgacct tgaaggggat 
     2041 ccaccggtcg ccaccatggc ggaaggatcc gtcgccaggc agcctgacct cttgacctgc 
     2101 gaacatgaag agatccacct cgccggctcg atccagccgc atggcgcgct tctggtcgtc 
     2161 agcgaacatg atcatcgcgt catccaggcc agcgccaacg ccgcggaatt tctgaatctc 
     2221 ggaagcgtac tcggcgttcc gctcgccgag atcgacggcg atctgttgat caagatcctg 
     2281 ccgcatctcg atcccaccgc cgaaggcatg ccggtcgcgg tgcgctgccg gatcggcaat 
     2341 ccctctacgg agtactgcgg tctgatgcat cggcctccgg aaggcgggct gatcatcgaa 
     2401 ctcgaacgtg ccggcccgtc gatcgatctg tcaggcacgc tggcgccggc gctggagcgg 
     2461 atccgcacgg cgggttcact gcgcgcgctg tgcgatgaca ccgtgctgct gtttcagcag 
     2521 tgcaccggct acgaccgggt gatggtgtat cgtttcgatg agcaaggcca cggcctggta 
     2581 ttctccgagt gccatgtgcc tgggctcgaa tcctatttcg gcaaccgcta tccgtcgtcg 
     2641 ctggtcccgc agatggcgcg gcagctgtac gtgcggcagc gcgtccgcgt gctggtcgac 
     2701 gtcacctatc agccggtgcc gctggagccg cggctgtcgc cgctgaccgg gcgcgatctc 
     2761 gacatgtcgg gctgcttcct gcgctcgatg tcgccgatcc atctgcagtt cctgaaggac 
     2821 atgggcgtgc gcgccaccct ggcggtgtcg ctggtggtcg gcggcaagct gtggggcctg 
     2881 gttgtctgtc accattatct gccgcgcttc atccgtttcg agctgcgggc gatctgcaaa 
     2941 cggctcgccg aaaggatcgc gacgcggatc accgcgcttg agagctaagc ggccgcgact 
     3001 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc 
     3061 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat 
     3121 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 
     3181 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa 
     3241 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt 
     3301 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag 
     3361 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 
     3421 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat 
     3481 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 
     3541 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 
     3601 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac 
     3661 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc 
     3721 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 
     3781 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa 
     3841 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 
     3901 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 
     3961 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 
     4021 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 
     4081 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 
     4141 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag 
     4201 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 
     4261 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 
     4321 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 
     4381 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg 
     4441 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 
     4501 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 
     4561 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 
     4621 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 
     4681 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 
     4741 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga 
     4801 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 
     4861 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 
     4921 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 
     4981 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga 
     5041 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag 
     5101 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct 
     5161 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat 
     5221 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg 
     5281 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc 
     5341 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag 
     5401 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc 
     5461 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 
     5521 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 
     5581 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 
     5641 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 
     5701 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 
     5761 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 
     5821 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 
     5881 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 
     5941 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 
     6001 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 
     6061 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 
     6121 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 
     6181 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 
     6241 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 
     6301 taaccgtatt accgccatgc at 
//
