LOCUS       pVimentin-miRFP703        6340 bp    DNA     circular      7-MAY-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12278346|
COMMENT     VNTDBDATE|-12278346|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pVimentin-miRFP703|
COMMENT     VNTAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     misc_feature    61..568
                     /vntifkey="21"
                     /label=CMV
     misc_feature    4236..5030
                     /vntifkey="21"
                     /label=NeoR/KanR
     misc_feature    637..2034
                     /vntifkey="21"
                     /label=Vimentin
     misc_feature    2056..3003
                     /vntifkey="21"
                     /label=miRFP713
BASE COUNT     1486 a      1764 c      1707 g      1383 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attcccatgt ccaccaggtc cgtgtcctcg 
      661 tcctcctacc gcaggatgtt cggcggcccg ggcaccgcga gccggccgag ctccagccgg 
      721 agctacgtga ctacgtccac ccgcacctac agcctgggca gcgcgctgcg ccccagcacc 
      781 agccgcagcc tctacgcctc gtccccgggc ggcgtgtatg ccacgcgctc ctctgccgtg 
      841 cgcctgcgga gcagcgtgcc cggggtgcgg ctcctgcagg actcggtgga cttctcgctg 
      901 gccgacgcca tcaacaccga gttcaagaac acccgcacca acgagaaggt ggagctgcag 
      961 gagctgaatg accgcttcgc caactacatc gacaaggtgc gcttcctgga gcagcagaat 
     1021 aagatcctgc tggccgagct cgagcagctc aagggccaag gcaagtcgcg cctgggggac 
     1081 ctctacgagg aggagatgcg ggagctgcgc cggcaggtgg accagctaac caacgacaaa 
     1141 gcccgcgtcg aggtggagcg cgacaacctg gccgaggaca tcatgcgcct ccgggagaaa 
     1201 ttgcaggagg agatgcttca gagagaggaa gccgaaaaca ccctgcaatc tttcagacag 
     1261 gatgttgaca atgcgtctct ggcacgtctt gaccttgaac gcaaagtgga atctttgcaa 
     1321 gaagagattg cctttttgaa gaaactccac gaagaggaaa tccaggagct gcaggctcag 
     1381 attcaggaac agcatgtcca aatcgatgtg gatgtttcca agcctgacct cacggctgcc 
     1441 ctgcgtgacg tacgtcagca atatgaaagt gtggctgcca agaacctgca ggaggcagaa 
     1501 gaatggtaca aatccaagtt tgctgacctc tctgaggctg ccaaccggaa caatgacgcc 
     1561 ctgcgccagg caaagcagga gtccactgag taccggagac aggtgcagtc cctcacctgt 
     1621 gaagtggatg cccttaaagg aaccaatgag tccctggaac gccagatgcg tgaaatggaa 
     1681 gagaactttg ccgttgaagc tgctaactac caagacacta ttggccgcct gcaggatgag 
     1741 attcagaata tgaaggagga aatggctcgt caccttcgtg aataccaaga cctgctcaat 
     1801 gttaagatgg cccttgacat tgagattgcc acctacagga agctgctgga aggcgaggag 
     1861 agcaggattt ctctgcctct tccaaacttt tcctccctga acctgaggga aactaatctg 
     1921 gattcactcc ctctggttga tacccactca aaaaggacac ttctgattaa gacggttgaa 
     1981 actagagatg gacaggttat caacgaaact tctcagcatc acgatgacct tgaaggggat 
     2041 ccaccggtcg ccaccatggc ggaaggctcc gtcgccaggc agcctgacct cttgacctgc 
     2101 gacgatgagc cgatccatat ccccggtgcc atccaaccgc atggactgct gctcgccctc 
     2161 gccgccgaca tgacgatcgt tgccggcagc gacaaccttc ccgaactcac cggactggcg 
     2221 atcggcgccc tgatcggccg ctctgcggcc gatgtcttcg actcggagac gcacaaccgt 
     2281 ctgacgatcg ccttggccga gcccggggcg gccgtcggag caccgatcac tgtcggcttc 
     2341 acgatgcgaa aggacgcagg cttcatcggc tcctggcatc gccatgatca gctcatcttc 
     2401 ctcgagctcg agcctcccca gcgggacgtc gccgagccgc aggcgttctt ccgccgcacc 
     2461 aacagcgcca tccgccgcct gcaggccgcc gaaaccttgg aaagcgcctg cgccgccgcg 
     2521 gcgcaagagg tgcggaagat taccggcttc gatcgggtga tgatctatcg cttcgcctcc 
     2581 gacttcagcg gcgaagtgat cgcagaggat cggtgcgccg aggtcgagtc aaaactaggc 
     2641 ctgcactatc ctgcctcaac cgtgccggcg caggcccgtc ggctctatac catcaacccg 
     2701 gtacggatca ttcccgatat caattatcgg ccggtgccgg tcaccccaga cctcaatccg 
     2761 gtcaccgggc ggccgattga tcttagcttc gccatcctgc gcagcgtctc gcccgtccat 
     2821 ctggagttca tgcgcaacat aggcatgcac ggcacgatgt cgatctcgat tttgcgcggc 
     2881 gagcgactgt ggggattgat cgtttgccat caccgaacgc cgtactacgt cgatctcgat 
     2941 ggccgccaag cctgcaagag ggtcgccgag aggctggcca ctcagatcgg cgtgatggaa 
     3001 gagtaagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact 
     3061 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt 
     3121 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     3181 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     3241 tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt 
     3301 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa 
     3361 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa 
     3421 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac 
     3481 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga 
     3541 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa 
     3601 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc 
     3661 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca 
     3721 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 
     3781 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 
     3841 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 
     3901 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg 
     3961 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 
     4021 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 
     4081 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 
     4141 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 
     4201 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 
     4261 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 
     4321 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 
     4381 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt 
     4441 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 
     4501 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 
     4561 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 
     4621 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 
     4681 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 
     4741 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg 
     4801 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 
     4861 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 
     4921 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 
     4981 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct 
     5041 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac 
     5101 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 
     5161 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga 
     5221 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa 
     5281 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca 
     5341 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc 
     5401 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc 
     5461 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt 
     5521 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 
     5581 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 
     5641 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 
     5701 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 
     5761 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa 
     5821 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 
     5881 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 
     5941 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 
     6001 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 
     6061 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 
     6121 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 
     6181 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 
     6241 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 
     6301 atcccctgat tctgtggata accgtattac cgccatgcat 
//
