LOCUS pHAGE2_TetOminiC 8740 bp ds-DNA circular 20-AUG-2015 SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers LTR 1..636 /gene="LTR" /vntifkey="19" /note="Long Terminal Repeat" /label=5'\LTR /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 637..1155 /gene="psi" /product="pbs-gag" /vntifkey="21" /note="PSI Packaging Sequence" /label=PSI /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1303..1536 /gene="rre" /product="minimal RRE" /vntifkey="21" /note="Rev Responsive Element" /label=RRE /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2034..2211 /gene="cppt" /vntifkey="21" /note="Central Poly Purine Track" /label=HIV\cpPu\(Trip) /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5181..5772 /gene="WPRE" /vntifkey="21" /note="Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element" /label=WPRE /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 6354..7193 /gene="AMPr" /product="beta-lactamase (mature form)" /vntifkey="4" /note="Ampicillin Resistance Gene" /label=AMPr /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 7422..7955 /gene="ORI" /product="ColE1 origin of replication" /vntifkey="33" /note="Bacterial Origin of Replication" /label=ORI /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 8192..8315 /gene="SV40 ori" /vntifkey="21" /label=SV40\ori /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 8635..8663 /vntifkey="28" /label=pHAGE-S1-500 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 401..421 /vntifkey="28" /label=pHAGE-S501-1000 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 898..919 /vntifkey="28" /label=pHAGE-S1001-1500 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 1391..1417 /vntifkey="28" /label=pHAGE-S1501-2000 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 1898..1922 /vntifkey="28" /label=pHAGE-S2001-2500 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 5440..5460 /vntifkey="28" /label=pHAGE-S4001-4500 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 6130..6152 /vntifkey="28" /label=pHAGE-S6001-6500 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 6603..6627 /vntifkey="28" /label=pHAGE-S6501-7000 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 7123..7147 /vntifkey="28" /label=pHAGE-S7001-7500 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 7627..7651 /vntifkey="28" /label=pHAGE-S7501-8000 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 8130..8149 /vntifkey="28" /label=pHAGE-S8001-8500 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(158..179) /vntifkey="28" /label=pHAGE-A0050-8050 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(667..690) /vntifkey="28" /label=pHAGE-A0550-0050 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(1166..1189) /vntifkey="28" /label=pHAGE-A1050-0550 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(1650..1678) /vntifkey="28" /label=pHAGE-A1550-1050 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(2084..2112) /vntifkey="28" /label=pHAGE-A2050-1550 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(5287..5310) /vntifkey="28" /label=pHAGE-A3550-3050 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(6403..6426) /vntifkey="28" /label=pHAGE-A6050-5550 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(6872..6895) /vntifkey="28" /label=pHAGE-A6550-6050 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(7383..7404) /vntifkey="28" /label=pHAGE-A7050-6550 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(7894..7915) /vntifkey="28" /label=pHAGE-A7550-7050 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(8370..8396) /vntifkey="28" /label=pHAGE-A8050-7550 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 455..551 /vntifkey="21" /note="HIV Repeat Region" /label=HIV\R /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2685..3641 /label=mSox2 /ApEinfo_fwdcolor=#000080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 636..658 /vntifkey="21" /note="tRNA binding site for Lysine tRNA" /label=PBS-K /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 697..806 /vntifkey="21" /label=HIV-PSI /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3744..5165 /label=mKlf-4 /ApEinfo_fwdcolor=#008000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 807..1144 /vntifkey="21" /note="HIV gag sequence 351bp to 688bp" /label=HR-ePSI /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 552..636 /vntifkey="21" /note="HIV U5" /label=HIV\U5 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1..454 /vntifkey="21" /note="Full Length HIV U3" /label=HIV\U3 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 6301..6335 /vntifkey="30" /note="Beta Lactamase Promoter" /label=P-Bla /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(5209..5243) /vntifkey="28" /label=pHAGE\3'\CDS /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5777..5878 /vntifkey="21" /note="HIV nef" /label=HIV\nef /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5919..6015 /vntifkey="21" /label=HIV\R(1) /ApEinfo_label=HIV\R /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6016..6098 /vntifkey="21" /note="HIV U5" /label=HIV\U5(1) /ApEinfo_label=HIV\U5 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5827..5841 /vntifkey="21" /note="PolyPurine Tract" /label=HIV\PPT /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 mutation 833..834 /vntifkey="62" /note="Filled in Cla Site from original HIV genome to disrupt gag polyprotein ORF" /label=Fill-in /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 LTR 5777..6098 /vntifkey="19" /label=3'\LTR /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 splicing_signal 744..745 /vntifkey="38" /note="Major 5' Splice Donor 289" /label=5'\SD\289 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 splicing_signal 1911..1912 /vntifkey="38" /label=SA\7925 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 splicing_signal 2188..2189 /vntifkey="38" /label=SA\4459 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 mutation 1357..1357 /vntifkey="62" /note="This G is a C in the WT HIV-1 genome" /label=RRE\Mutation /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2014..2027 /vntifkey="21" /note="Linker Junk Sequence probably left over from cloning" /label=Junk /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1156..2013 /vntifkey="4" /note="HIV env sequence from bp7168 to 8025 (contains RRE) Cloned from BglII to BamHI out of HIV" /label=env /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 790..1144 /vntifkey="4" /note="HIV gag sequence from 336bp to 688bp (Clone to PvuII site in HIV and filled in ClaI digest to take gag CDS out of frame)" /label=HIV\gag /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 5'UTR 551..635 /vntifkey="52" /note="HIV 5' UTR" /label=5'\UTR /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 853..855 /vntifkey="21" /note="Stop codon in gag put in frame by ClaI fill in" /label=Stop\Codon /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1245..1593 /vntifkey="21" /note="Rev Responsive Element according to Belasco in Molecular Cell, Vol. 7, 603-614, March, 2001" /label=RRE(1) /ApEinfo_label=RRE /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5844..5880 /vntifkey="21" /note="1st Half of deleted U3" /label=dU3 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5900..5918 /vntifkey="21" /note="2nd Half of deleted U3" /label=dU3(1) /ApEinfo_label=dU3 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 stem_loop 697..731 /vntifkey="39" /note="PSI Stem loop 1" /label=SL-1 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 stem_loop 736..754 /vntifkey="39" /note="PSI Stem loop 2" /label=SL-2 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 stem_loop 766..779 /vntifkey="39" /note="PSI Stem Loop 3" /label=SL-3 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 stem_loop 793..806 /vntifkey="39" /note="PSI Stem Loop 4" /label=SL-4 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 polyA_site 550..550 /vntifkey="26" /note="Exact base to which Poly-A is added" /label=pA\Site /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 polyA_signal 570..577 /vntifkey="25" /note="GT Cluster shown to be necessary for Poly-A site activity by Cullen in J Virol. 1989 January; 63(1):421-424" /label=G-T\cluster /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 2556..2675 /vntifkey="29" /label=miniCMV /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2238..2555 /vntifkey="21" /note="Tetracyclin responsive element (TRE)" /label=TetO /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#000000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 7291..7973 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding 8436..8458 /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 6534..7193 /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5485..5564 /label=WPRE(1) /ApEinfo_label=WPRE /ApEinfo_fwdcolor=#a5c4b3 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2062..2076 /label=cPPT /ApEinfo_fwdcolor=#a596b4 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5827..5841 /label=cPPT(1) /ApEinfo_label=cPPT /ApEinfo_fwdcolor=#a596b4 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca 2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt 2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta 2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt 2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac 2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag 2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat 2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatgtat aacatgatgg 2701 agacggagct gaagccgccg ggcccgcagc aagcttcggg gggcggcggc ggaggaggca 2761 acgccacggc ggcggcgacc ggcggcaacc agaagaacag cccggaccgc gtcaagaggc 2821 ccatgaacgc cttcatggta tggtcccggg ggcagcggcg taagatggcc caggagaacc 2881 ccaagatgca caactcggag atcagcaagc gcctgggcgc ggagtggaaa cttttgtccg 2941 agaccgagaa gcggccgttc atcgacgagg ccaagcggct gcgcgctctg cacatgaagg 3001 agcacccgga ttataaatac cggccgcggc ggaaaaccaa gacgctcatg aagaaggata 3061 agtacacgct tcccggaggc ttgctggccc ccggcgggaa cagcatggcg agcggggttg 3121 gggtgggcgc cggcctgggt gcgggcgtga accagcgcat ggacagctac gcgcacatga 3181 acggctggag caacggcagc tacagcatga tgcaggagca gctgggctac ccgcagcacc 3241 cgggcctcaa cgctcacggc gcggcacaga tgcaaccgat gcaccgctac gacgtcagcg 3301 ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg cccacctaca 3361 gcatgtccta ctcgcagcag ggcacccccg gtatggcgct gggctccatg ggctctgtgg 3421 tcaagtccga ggccagctcc agcccccccg tggttacctc ttcctcccac tccagggcgc 3481 cctgccaggc cggggacctc cgggacatga tcagcatgta cctccccggc gccgaggtgc 3541 cggagcccgc tgcgcccagt agactgcaca tggcccagca ctaccagagc ggcccggtgc 3601 ccggcacggc cattaacggc acactgcccc tgtcgcacat ggcatgcggc tccggcgagg 3661 gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggccca ctcgagatga 3721 ggcagccacc tggcgagtct gacatggctg tcagcgacgc tctgctcccg tccttctcca 3781 cgttcgcgtc cggcccggcg ggaagggaga agacactgcg tccagcaggt gccccgacta 3841 accgttggcg tgaggaactc tctcacatga agcgacttcc cccacttccc ggccgcccct 3901 acgacctggc ggcgacggtg gccacagacc tggagagtgg cggagctggt gcagcttgca 3961 gcagtaacaa cccggccctc ctagcccgga gggagaccga ggagttcaac gacctcctgg 4021 acctagactt tatcctttcc aactcgctaa cccaccagga atcggtggcc gccaccgtga 4081 ccacctcggc gtcagcttca tcctcgtctt ccccagcgag cagcggccct gccagcgcgc 4141 cctccacctg cagcttcagc tatccgatcc gggccggggg tgacccgggc gtggctgcca 4201 gcaacacagg tggagggctc ctctacagcc gagaatctgc gccacctccc acggccccct 4261 tcaacctggc ggacatcaat gacgtgagcc cctcgggcgg cttcgtggct gagctcctgc 4321 ggccggagtt ggacccagta tacattccgc cacagcagcc tcagccgcca ggtggcgggc 4381 tgatgggcaa gtttgtgctg aaggcgtctc tgaccacccc tggcagcgag tacagcagcc 4441 cttcggtcat cagtgttagc aaaggaagcc cagacggcag ccaccccgtg gtagtggcgc 4501 cctacagcgg tggcccgccg cgcatgtgcc ccaagattaa gcaagaggcg gtcccgtcct 4561 gcacggtcag ccggtcccta gaggcccatt tgagcgctgg accccagctc agcaacggcc 4621 accggcccaa cacacacgac ttccccctgg ggcggcagct ccccaccagg actaccccta 4681 cactgagtcc cgaggaactg ctgaacagca gggactgtca ccctggcctg cctcttcccc 4741 caggattcca tccccatccg gggcccaact accctccttt cctgccagac cagatgcagt 4801 cacaagtccc ctctctccat tatcaagagc tcatgccacc gggttcctgc ctgccagagg 4861 agcccaagcc aaagagggga agaaggtcgt ggccccggaa aagaacagcc acccacactt 4921 gtgactatgc aggctgtggc aaaacctata ccaagagttc tcatctcaag gcacacctgc 4981 gaactcacac aggcgagaaa ccttaccact gtgactggga cggctgtggg tggaaattcg 5041 cccgctccga tgaactgacc aggcactacc gcaaacacac agggcaccgg ccctttcagt 5101 gccagaagtg cgacagggcc ttttccaggt cggaccacct tgccttacac atgaagaggc 5161 acttttaaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact 5221 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 5281 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 5341 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 5401 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 5461 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 5521 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca 5581 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 5641 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 5701 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc 5761 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 5821 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatca 5881 cctgcaggac aggcgcgccc tgctttttgc ttgtactggg tctctctggt tagaccagat 5941 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 6001 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 6061 cctcagaccc ttttagtcag tgtggaaaat ctctagcacc cgggcgatta aggaaagggc 6121 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 6181 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 6241 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 6301 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 6361 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 6421 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 6481 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 6541 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac 6601 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 6661 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 6721 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 6781 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 6841 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 6901 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 6961 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 7021 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 7081 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 7141 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 7201 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 7261 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 7321 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 7381 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 7441 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 7501 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 7561 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 7621 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 7681 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 7741 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 7801 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 7861 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 7921 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 7981 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 8041 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 8101 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 8161 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat 8221 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 8281 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga 8341 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 8401 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 8461 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc 8521 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga 8581 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca 8641 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa 8701 gaaattgtat ttgttaaata tgtactacaa acttagtagt //