LOCUS       Exported                7602 bp ds-DNA     circular SYN 30-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Patapoutian Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 25, 2019 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    609..629
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    641..2956
                     /label=AtCSC1
     misc_feature    2964..2968
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    2970..3556
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3557..4267
                     /codon_start=1
                     /product="mCherry"
                     /label=mCherry
                     /note="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    4390..4511
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4518..4973)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5000..5104
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5106..5463
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5314..5449
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5498..6292
                     /codon_start=1
                     /gene="Neo/KanR"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6524..6571
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6900..7488
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcctcgag atggctaccc tgcaagatat
      661 cggcgtgagc gctggaatca acatcctctc cgcctttgtg ttcttcatca tcttcgctgt
      721 gctgagactg cagcccttta acgacagggt ctacttcagc aagtggtacc tgaagggact
      781 caggtccagc cctgctaggg gaggagcctt cgctcagaga ttcgtgaacc tcgacttcag
      841 gagctacatg aagttcctga actggatgcc tgaggctctc aaaatgcccg agcccgagct
      901 gattgatcac gccggcctgg atagcgtcgt ctatctgagg atctactggc tcggcctgaa
      961 gatctttaca cctatcgccg tgctggcctg ggctgtcctg gtgcccgtga attggaccaa
     1021 caacacactg gagatggcca aacagctgag gaatgtgacc tcctccgaca tcgacaaact
     1081 gagcgtctcc aacatccccg agtatagcat gaggttctgg acccacatcg tcatggccta
     1141 cgcctttacc atctggacct gctacgtcct gatgaaggag tacgagacca tcgccaacat
     1201 gaggctgcag ttcgtggcca gcgaagctag aaggcccgac cagtttaccg tgctggtgag
     1261 gaacgtgccc cctgacgctg acgaatccgt gagcgagctc gtcgagcatt tcttcctggt
     1321 gaaccatccc gaccactacc tgacccacca agtggtgtgc aacgccaaca aactcgccga
     1381 cctggtcaaa aaaaaaaaga agctccagaa ctggctggac tactaccagc tcaagtacgc
     1441 caggaacaat agccagagga ttatggtcaa gctcggcttt ctgggcctct ggggccagaa
     1501 ggtcgatgcc attgagcact acatcgccga gattgacaaa atctccaagg agatctccaa
     1561 agagagggag gaagtggtga acgaccccaa agccatcatg cctgccgcct tcgtgagctt
     1621 taagaccagg tgggctgccg ccgtgtgcgc tcagacccaa cagaccagaa accccaccca
     1681 gtggctcacc gaatgggctc ctgaacccag ggacgtgttc tggtccaatc tggccatccc
     1741 ctatgtgagc ctgaccgtga ggaggctgat tatgcacgtg gccttcttct tcctgacctt
     1801 tttctttatc gtccctatcg ccttcgtcca gtccctggct accatcgaag gaattgtcaa
     1861 agccgccccc ttcctcaagt tcattgtcga cgacaagttc atgaagagcg tgatccaggg
     1921 attcctccct ggcattgctc tgaagctgtt cctggctttt ctcccctcca tcctgatgat
     1981 catgagcaag ttcgagggct tcacctccat ctccagctta gaaaggaggg ccgccttcag
     2041 atactatatt tttaacctgg tgaatgtctt tctggcctcc gtgattgctg gagccgcctt
     2101 cgagcagctc aacagcttcc tcaaccagtc cgctaaccag attcctaaga ccatcggcgt
     2161 ggctatcccc atgaaagcca cattctttat cacatacatt atggtggacg gatgggccgg
     2221 cgtggctgga gaaatcctca tgctgaagcc tctgattatg tttcatctga aaaatgcttt
     2281 tctggtgaag acagacaagg atagggagga agccatggac cctggttcta ttggctttaa
     2341 cacaggcgag cccaggattc agctgtactt cctgctggga ctcgtctacg cccccgtgac
     2401 acccatgctg ctgcccttta tcctcgtgtt cttcgctctg gcctacattg tctacaggca
     2461 ccagatcatc aacgtgtaca atcaggagta cgaatccgcc gctgccttct ggcccgacgt
     2521 gcatggcaga gtgattgctg ccctggtgat cagccagctg ctcctcatgg gactgctcgg
     2581 aaccaagcat gccgctctgg ccgccccctt tctgattgct ctgcccgtcc tgaccatcgg
     2641 ctttcaccat ttctgcaagg gcaggtacga gcctgctttc atcaggtacc ccctgcagga
     2701 agccatgatg aaggacacct tagaaacagc cagagagcct aacctcaacc tcaagggcta
     2761 cctgcagaat gcctacgtgc accccgtgtt caagggcgac gaggatgact atgacatcga
     2821 tgacaaactg ggcaagtttg aggacgaggc tatcatcgtc cccaccaaaa ggcaatccag
     2881 gagaaacacc cctgccccca gcattatctc cggcgatgat agccctagcc tgcccttttc
     2941 cggcaagctc gtgtgaccgc ggggatccgc ccctctccct cccccccccc taacgttact
     3001 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     3061 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     3121 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     3181 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     3241 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     3301 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     3361 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     3421 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     3481 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     3541 taatatggcc acaaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     3601 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     3661 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     3721 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     3781 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     3841 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     3901 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     3961 ctccgacggc cccgtaatgc agaagaaaac catgggctgg gaggcctcct ccgagcggat
     4021 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     4081 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     4141 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     4201 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     4261 caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
     4321 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     4381 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4441 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4501 aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     4561 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     4621 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     4681 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4741 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4801 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     4861 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     4921 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     4981 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5041 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5101 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5161 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5221 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5281 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5341 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5401 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5461 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     5521 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     5581 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     5641 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     5701 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5761 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     5821 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5881 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5941 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     6001 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     6061 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     6121 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     6181 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     6241 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     6301 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     6361 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6421 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     6481 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     6541 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     6601 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     6661 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     6721 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     6781 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6841 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6901 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6961 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7021 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     7081 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7141 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7201 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7261 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7321 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7381 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7441 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7501 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7561 ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
