LOCUS       Exported                7695 bp ds-DNA     circular SYN 25-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pIRES2-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 25, 2019 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    635..3055
                     /label=OSCA1.8
     misc_feature    3063..3649
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3650..4360
                     /codon_start=1
                     /product="mCherry"
                     /label=mCherry
                     /note="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    4483..4604
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4611..5066)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5093..5197
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5199..5556
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5407..5542
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5591..6385
                     /codon_start=1
                     /gene="Neo/KanR"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6617..6664
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6993..7581
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcatggcc actttacaag acattggcgt
      661 ctccgctctg atcaatctct tcggcgcctt tttattttta atcgctttcg ccgtgctgcg
      721 tattcaaccc atcaacgatc gtgtgtactt ccccaagtgg tatttaactg gtgagagaaa
      781 cagccctagg agaagcgaca gaactttagt cggcaaattc gtcaatttaa attataaaac
      841 ctacttcacc tttttaaatt ggatgcccca agctatgaaa atgagcgaat ccgagattat
      901 cagacacgcc ggactcgaca gcgccatctt tttaaggatt tacactttag gactgaagat
      961 ctttgccccc gttatggtgc tggctctcgt ggtcctcgtc cccgttaatg tgagctccgg
     1021 cactttattc tttctcaaga aggaactggt ggtgagcaac atcgataagc tctccatcag
     1081 caacgtgcag cctaagagct ccaagttctt cttccacatc gccgtggagt atatttttac
     1141 cttctgggct tgttttatgc tctatcgtga gtacaacaat gtggccatca tgaggctgca
     1201 gtatttagcc tcccagagga ggaggcccga acagttcaca gtggtggtga gaaatgtccc
     1261 cgacatgccc ggccattccg tgcccgatac cgtggatcaa tttttcaaaa ccaatcaccc
     1321 cgagcactat ttatgccatc aagctgtgta caatgccaat acctacgcca agctggtcaa
     1381 gcagagggcc aagctgcaga ggtggttcga ctactatgtt ttaaagcacc agaggaaccc
     1441 ccacaagcag cctacatgta ggaccggatt tctcggcctc tggggcaaga gggtggactc
     1501 catcgagtat tataaacaac agatcaagga gtttgaccat aacatgagcc tcgagaggca
     1561 gaaggtttta aaggactcca agctgatgct gcccgttgcc tttgtgagct ttgactctcg
     1621 ttggggagct gccgtgtgcg cccagaccca acagagcaag aatcccacac tgtggctgac
     1681 cagctccgcc cccgaaccta gagacatcta ctggcagaat ttagccatcc ccttcatctc
     1741 cctcacaatt cgtaagctgg tcattggcgt gagcgtgttc gccctcgtgt ttttctacat
     1801 gattcccatc gccttcgtgc agagcctcgc caatctggag ggcctcgata gagtggctcc
     1861 cttcctcaga cccgtcacaa ggctcgactt catcaagtcc tttttacaag gttttctgcc
     1921 cggactggcc ctcaaaatct tcctctggat tttacctaca gtcttattaa tcatgagcaa
     1981 gattgagggc tatattgctt taagcacact ggaaaggagg gccgctgcca agtactatta
     2041 cttcatgctg gtcaacgtct ttttaggaag cattatcgct ggcaccgcct tcgaacagct
     2101 gcactccttt ctccaccagt cccctagcca gattcctcgt acaatcggcg ttagcattcc
     2161 catgaaagcc accttcttca ttacctacat catggtggat ggatgggctg gcatcgccgg
     2221 agagatttta aggctgaaac ctctggtcat cttccatctg aaaaacatgt tcatcgtgaa
     2281 gaccgaagag gacagagtga gagccatgga ccccggtttt gtcgacttca aggagacaat
     2341 cccctcttta cagctgtact ttctgctggg cattgtctat accgccgtga cccctatttt
     2401 actgcccttt attctgattt tcttcgcttt cgcctatctc gtgtatcgtc atcagatcat
     2461 caatgtgtat aaccagcagt acgagtcttg tggagccttc tggcctcacg tgcacggtcg
     2521 tatcatcgct tctttactca ttagccaact gttattaatg ggtttactgg ccagcaagaa
     2581 agctgccgat tccacccctt tactgatcat cctccccatt ttaactttat ccttccataa
     2641 gtactgcaaa cacagatttg agcccgcctt tcgtcagtac cctttagagg aggccatggc
     2701 caaagacaag ctggaaaagg agaccgagcc cgaactgaac atgaaggctg atttagccga
     2761 cgcctacctc caccctatct tccacagctt cgagaaagaa gtggagctga gcagcagcag
     2821 ctccagcgaa aaggaaaccc atcaagaaga gacccccgaa gtgagagtcg acaagcatga
     2881 gacccagtcc tcctcccccg ttaccgagct gggcacaagc agccaccacc accacgtgta
     2941 caacagcaca tccccctcct cccactacgc cagcgcttat gagcagagca gctcccaata
     3001 cgaataccac tacaataccc accagtacga ggaacatgag tacagatata attgaggatc
     3061 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     3121 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     3181 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     3241 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     3301 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     3361 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     3421 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     3481 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     3541 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     3601 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tggtgagcaa
     3661 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     3721 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     3781 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     3841 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     3901 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     3961 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     4021 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     4081 aaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     4141 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     4201 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     4261 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     4321 ccgccactcc accggcggca tggacgagct gtacaagtaa agcggccgcg actctagatc
     4381 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     4441 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     4501 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4561 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag
     4621 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     4681 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     4741 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     4801 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     4861 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     4921 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     4981 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     5041 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     5101 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5161 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     5221 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5281 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     5341 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5401 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5461 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     5521 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
     5581 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5641 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5701 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5761 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5821 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5881 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5941 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     6001 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     6061 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     6121 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     6181 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     6241 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     6301 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6361 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     6421 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6481 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6541 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     6601 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     6661 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     6721 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     6781 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     6841 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6901 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6961 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7021 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7081 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     7141 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7201 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7261 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7321 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7381 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7441 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7501 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7561 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7621 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7681 attaccgcca tgcat
//
