LOCUS       Exported                7461 bp ds-DNA     circular SYN 11-JUL-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Patapoutian Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 19, 2019 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    609..629
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    641..2815
                     /label=ERD4
     misc_feature    1778..1792
                     /label=Chimera swap site
     misc_feature    2823..2827
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    2829..3415
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3416..4126
                     /codon_start=1
                     /product="mCherry"
                     /label=mCherry
                     /note="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    4249..4370
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4377..4832)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4859..4963
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4965..5322
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5173..5308
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5357..6151
                     /codon_start=1
                     /gene="Neo/KanR"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6383..6430
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6759..7347
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcctcgag atggagtttg gcagcttcct
      661 ggtgagcctg ggcacctcct tcgtgatctt cgtgatcctg atgctgctgt tcacatggct
      721 gagcaggaaa agcggcaacg ctcccatcta ctaccccaac cgtattttaa agggcctcga
      781 accttgggag ggcacatccc tcacaaggaa ccccttcgcc tggatgaggg aggccctgac
      841 ctccagcgag caggacgtgg tgaacctgtc cggagtggac accgccgtcc actttgtgtt
      901 cctctccacc gtgctgggca ttttcgcctg ttcctccctc ctgctgctgc ccacactgct
      961 ccctctcgct gccacagaca ataacattaa gaacaccaaa aacgccacag acaccacaag
     1021 caagggcaca ttctcccagc tggacaacct gtccatggcc aacatcacca agaagagctc
     1081 cagactctgg gctttcctgg gcgccgtgta ctggatctcc ctggtgacct acttcttcct
     1141 gtggaaggct tacaaacatg tgtcctccct cagggcccag gccctcatgt ccgctgacgt
     1201 gaaacctgaa caattcgcca tcctggtgag agacatgcct gcccctcccg acggacagac
     1261 ccagaaggag tttattgaca gctacttcag ggagatctac cccgagacct tctacagaag
     1321 cctggtggcc accgaaaaca gcaaggtcaa caaaatctgg gagaagctcg aaggctataa
     1381 gaagaagctc gctagagccg aggccatcct ggctgctaca aataacaggc ccaccaacaa
     1441 aaccggcttc tgtggcctgg tcggaaagca ggtggactcc atcgagtact acacagagct
     1501 cattaacgag tccgtcgcca aattagaaac cgagcagaaa gccgtgctgg ccgagaagca
     1561 gcagacagct gccgtcgtgt tcttcaccac cagggtggct gctgcctccg ctgctcagag
     1621 cctccattgc cagatggtcg acaagtggac cgtcaccgag gctcctgagc ccaggcagct
     1681 gctgtggcaa aacctgaaca tcaagctgtt cagcaggatc atcaggcaat atttcattta
     1741 cttcttcgtg gccgtgacaa ttctgttcta catgattccc atcgccttcg tgtccgctat
     1801 taccaccctg aaaaacctcc agaggatcat tcccttcatc aagcctgtgg tcgagattac
     1861 agccatcagg acagtgctgg agagcttcct gcctcagatc gccctgatcg tgttcctggc
     1921 catgctgccc aagctgctgc tgttcctgtc caaggccgag ggcattccta gccaatccca
     1981 cgccatcagg gctgccagcg gcaagtattt ctacttcagc gtcttcaacg tgtttatcgg
     2041 cgtgaccctg gccggaacac tgttcaatac cgtgaaggat attgctaaga accccaagct
     2101 ggacatgatc atcaatctgc tcgccaccag cctgcctaaa agcgccacat tcttcctgac
     2161 ctatgtcgcc ctcaagttct tcatcggcta cggcctggag ctgagcagaa tcatccccct
     2221 gatcattttc cacctgaaaa agaagtatct gtgcaaaacc gaggccgaag tgaaggaggc
     2281 ttggtaccct ggcgacctgt cctacgccac cagagtgccc ggcgatatgc tcattctgac
     2341 catcacattc tgctacagcg tgattgcccc cctcatcctg atcttcggca tcacatactt
     2401 cggcctggga tggctggtgc tgaggaatca ggccctgaag gtgtacgtcc ctagctacga
     2461 atcctacgga aggatgtggc ctcacatcca ccagagaatc ctggccgccc tgtttctgtt
     2521 tcaggtggtg atgtttggct atctgggcgc caaaaccttc ttctacaccg ccctcgtgat
     2581 ccccctgatc atcaccagcc tgattttcgg atacgtgtgc aggcagaagt tctacggcgg
     2641 cttcgagcac accgctctgg aggtggcttg cagagaactc aagcaatccc ccgacctgga
     2701 ggagatcttc agagcctaca ttcctcacag cctgtccagc cacaaacccg aagagcacga
     2761 gttcaagggc gccatgagca gataccagga cttcaacgcc atcgccggcg tgtgaccgcg
     2821 gggatccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     2881 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     2941 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     3001 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     3061 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     3121 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     3181 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     3241 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     3301 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     3361 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt
     3421 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     3481 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3541 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     3601 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     3661 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3721 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     3781 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     3841 gaagaaaacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     3901 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     3961 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     4021 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     4081 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaaagcg gccgcgactc
     4141 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     4201 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     4261 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     4321 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     4381 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     4441 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     4501 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     4561 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     4621 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     4681 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     4741 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     4801 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     4861 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4921 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     4981 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     5041 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5101 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5161 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5221 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     5281 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     5341 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     5401 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     5461 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     5521 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     5581 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     5641 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     5701 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5761 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5821 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5881 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5941 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     6001 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     6061 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     6121 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     6181 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     6241 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     6301 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     6361 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     6421 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     6481 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     6541 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     6601 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6661 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6721 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6781 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6841 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6901 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6961 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7021 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7081 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7141 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7201 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7261 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7321 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7381 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7441 aaccgtatta ccgccatgca t
//
