LOCUS       Exported                7728 bp ds-DNA     circular SYN 25-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pIRES2-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 25, 2019 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    629..3094
                     /label=OSCA4.1
     misc_feature    3096..3682
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3683..4393
                     /codon_start=1
                     /product="mCherry"
                     /label=mCherry
                     /note="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    4516..4637
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4644..5099)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5126..5230
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5232..5589
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5440..5575
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5624..6418
                     /codon_start=1
                     /gene="Neo/KanR"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6650..6697
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7026..7614
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcatgaac agaaacttta gccccgccgc
      661 catgcctcct attagcagca tgacaatcga caatagcttt tcccctcctc cttccagcgg
      721 agatctgccc gaaatccccg acgcttggta cggcaatatc caatacctcc tcaatatcag
      781 cgtgatcggt ttactgtgct gtgtcagcat cttcctcttc gtgaagctga ggagcgacca
      841 tcgtagaatg cccggcccta gcgccctctt ctccaagtta ttagccgtgt ggaaagccac
      901 atgtcgtgag atcgctagac actgcggcgc cgatgccgct cagttcttat taatcgaggg
      961 cggaagcttc gtgttattat tcagcattgc tgttttagcc gtgagcgtga tgctgccttt
     1021 aaatctgtat gctggcacag ctttactgag cgatgagctg tccaagacaa tgattaccca
     1081 cattcagaag ggcagcgccc tcctctggct gcacttcgtc tttgtggtca tcgtggtggt
     1141 gatctcccac tttggcatcg ctgctattga ggctcgtctg aagttcactc gttttaggga
     1201 tggcaacggc aatatcagcg accccaatgc caatagcacc gccgtgttta ccattatggt
     1261 gcaaggtctg cctaagaatc tgggaagcga tcgtgtggag ttcgaagatt gctttcgtct
     1321 gaagtacccc ggcaaagtgt acaagtttat cgtgcctatg gacctctgcg ctttagatga
     1381 tctggccacc gagctcgttc gtgttcgtga cgagattact tggctcgtgg ctaagatgga
     1441 ctctcgttta ttacccgatg agtatgagaa cgtcggcgac aatggtttag tgttctgcgt
     1501 gtgctcttta tgggttcgtg tcaaggtgct gtggagccaa atcaccgaga ggttcggctt
     1561 caccgacgat gaaaagctga ggaagctgca agaactgaga gccgatctcg agtcccaact
     1621 ggctgcctac aaagagggtc gtgcccaagg cgccggcgtc gctttcgtga tgtttaagga
     1681 cgtgtacacc gccaataagg ccgtgcaaga ttttcgtaac gagaggtctc gtaggactgg
     1741 taagtttttc agcgtgacag agttacgtct gcagaggaac cagtggaagg tcgatagggc
     1801 tcctttagcc acagacattt actggaacca tttaggactg accaaggtgg ctttaattgt
     1861 gagaagagtg atcgtgaaca ccattttact gctcatcctc gtgtttttta gctcccctct
     1921 cgctctgatt agcgctctcg tgtccgctgg cagaattttc aatgccgagg ctttagattc
     1981 cgctcagtac tggctgactt gggtgcagac cagcggatgg attggctctt taatcttcca
     2041 gtttctgccc aacgtgttta tcttcgtcag catgtatatc gtcatcccct ccgctttatc
     2101 ctatctgagc aagttcgaaa ggcatctgac agtcagcggc gagcagagag ccgctctgct
     2161 caagatggtg tgcttctttt tagtcaacct cattatcctc aaagctttag tggagtcctc
     2221 cctcgaatct gctttactga agatgagcag atgttacctc gacggcgaag actgcaaaag
     2281 gattgaggag tacatgagcc ccagctttct gtctcgtagc tgtgtctccg ctttagcttt
     2341 cctcatcacc tccaccttcc tcggcatcag cttcgattta ctggccccca tcccttggat
     2401 taagaagaag atccagaagt tcagaaagaa tgacatgctg cagctggtcc ccgaacagaa
     2461 cgaggaatac gctttagaaa accaagaacc ctcctccaat ttagagacac ctttactccc
     2521 cgagaacatg tttgagagcc ctaggttcgg agacatcgag cccatgtccc aagatttaag
     2581 cgagtaccct atcagcagaa ccagccccat ccccaagcag aagttcgact tcgctcagta
     2641 ctacgccttt aatttaacca tcttcgccct caccatgatc tacagctcct ttgccccttt
     2701 agtcgtcccc gttggagccg tgtatttcgg atatcgttac atcgtcgata aatacaactt
     2761 tttatacgtc tatcgtgtga gaggctttcc cgctggcaat gaaggaaagc tgatggacac
     2821 agttttatgt atcatgaggt tctgcgtgga cctctattta gtgagcatgt tattattctt
     2881 ctccgtcaag ggcgacagca ccaaactgca agctatcttc actttaggag ttttagtcat
     2941 gtacaaactg ctgcccagcg ataccgatcg ttatcatccc gccttactga ggagcattca
     3001 gaccgtggat agcatcatcg acggacccgt cgattacgag gcctacagcc accctaactt
     3061 cgactgggac acctacaaca atcgttgagg atccgcccct ctccctcccc cccccctaac
     3121 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3181 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3241 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3301 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     3361 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     3421 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     3481 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     3541 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     3601 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     3661 cgatgataat atggccacaa ccatggtgag caagggcgag gaggataaca tggccatcat
     3721 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     3781 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     3841 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     3901 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     3961 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     4021 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     4081 cttcccctcc gacggccccg taatgcagaa gaaaaccatg ggctgggagg cctcctccga
     4141 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     4201 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     4261 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     4321 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     4381 gctgtacaag taaagcggcc gcgactctag atcataatca gccataccac atttgtagag
     4441 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     4501 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4561 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4621 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     4681 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     4741 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     4801 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     4861 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4921 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4981 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     5041 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     5101 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5161 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5221 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     5281 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5341 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5401 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5461 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5521 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5581 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5641 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5701 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5761 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     5821 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5881 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5941 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     6001 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6061 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     6121 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     6181 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6241 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6301 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6361 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6421 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6481 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6541 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     6601 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     6661 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     6721 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     6781 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     6841 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     6901 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6961 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7021 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7081 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7141 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     7201 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7261 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7321 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7381 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7441 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7501 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7561 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7621 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7681 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
