LOCUS       Exported                7569 bp ds-DNA     circular SYN 25-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Patapoutian Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 25, 2019 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    609..629
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    641..2923
                     /label=dTmem63a
     misc_feature    2931..2935
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    2937..3523
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3524..4234
                     /codon_start=1
                     /product="mCherry"
                     /label=mCherry
                     /note="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    4357..4478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4485..4940)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4967..5071
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5073..5430
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5281..5416
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5465..6259
                     /codon_start=1
                     /gene="Neo/KanR"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6491..6538
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6867..7455
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcctcgag atggtgatgt ccgaaaattc
      661 taacatcagc tactgccagg ctgtcgccaa taggaccagc tttttcacca ccgcctacaa
      721 tggcatcccc gagaccctca tcctgaacac catcttttgg gtcctgctca tcctcctctt
      781 cacagtgctg aggcatcaag ccggagactt cggcagactg gccctggtga atagcaacgg
      841 cagcaagaag aggtggaccg agatcttcta tagcagagct gccagcgtgg ttgatcctca
      901 gcccagcaca agcacccagg tgagccccag gcccagcaca acaagccagg gcgtgcctga
      961 tagcaccccc ctgtccccca tccaacccga agagggcctc ttcggctgga tcagagtgac
     1021 actgaagctc aggaaggaga ccatcctgct gcatacagga cccgacgccg tgcactatct
     1081 cagcttccaa cagcacctga tggctgtcat ggccctggtc accatcgtga gcctggtgat
     1141 catcctgccc gtgaacttcc tgaacggccc taaggaaaac ccctatgacg tgaacgcctt
     1201 cggcaggacc acaatggcca acctgtcccc tgacagccct tggctgtggg tccacacaat
     1261 catcaccatt ctgtatatcc ccctcgtggt cctgatcatg aggagggcca gcggaaggaa
     1321 cgctttcaag aaagccgcca ccaggaccat catgatctcc aatatcagct ccagcgacag
     1381 gaacaagaca gtcgtgagga actacatgca ggagctgttc cccgatgtga caatcgagaa
     1441 cgtgagcatc gcctacaaca ttagcaggct ctacgtgaga aatgccgaat ttgagagagt
     1501 gcacgaggct aggctgtact gcgaacacca cagaaacaga gacaccctga tggtgaagcc
     1561 cgaaatgtgc tcctgcaaga aggagaacgc ctacgaatac tatcaaagag aggagaggaa
     1621 gctgtccggc gacgtggcca gactgagagc ctccaccatg aacgagcccc tcgatatcgc
     1681 tttcattacc gtgtccaccg tccaagaagc ccagaacatc gtgacccact tcacacccgg
     1741 aacctatagg cagtggcatc tcgtcttcgc cccttccccc gacgatctct tctgggagaa
     1801 tctcaacgtc aacaagagcc actggtatct gaaattcttc tgtgtcaacg ccgtgctgtt
     1861 cctggtcctg ttcttcctga gcacacccgc catggtcgtg agcctgctga atagcagacc
     1921 ctgggtgaag gagaccgagg gcaagatcag ccccctggtg gccgattttc tgcccaccct
     1981 gatgctgtgg accctgagcg ctctcatgcc tgtcatcgtc gccatcagcg ataagtggat
     2041 gagacactac accaggagca aacagaacta cagcattatg accaaatgct tcggctacct
     2101 cctgatgatg atcatgatcc tgccctccct gggactcacc agcgctcaag ctctcttaga
     2161 atggggcttc ctgaatcaga ccgacagatg gcagtgcatt ttcctccctg acaggggctc
     2221 cttctacgtg aactacatca ttaccgctgc ttttatcggc acagctctgg agctgctgag
     2281 attccctgag ctgatcgtct atatctggtc cctcctgaag gccaagagca aagccgagac
     2341 cccttacatc aggaaggcca tcctcatcga gttccccttc ggaacccact acgcctggac
     2401 aaccctggtg ttcaccatcg ctatcgtcta ttccgtggcc tgtcctctga ttatgccttt
     2461 cgccatgatc tacatctgcc tcaagcactt cgtcgacaga cacaatctgt acttcgctta
     2521 tggccccagc aacatgatta gcaggaaggg cggaaagatc catagcaccg ccgtcaccat
     2581 gaccaagttc tccgtcgtca ttctggtgct cgtcatggcc atgatttgct tcttcagggg
     2641 agacaacggc atggctaggt tcctgctgct catcatcagc ctggccatca ccctgacact
     2701 gttcaccttc atgagcccca tcaagagatg cgccgccacc aggaggcctt ccatcgtgga
     2761 aatcgccggc cccgccccta tctatgtgcc cgatgtgctg aaggataacg gcatcaggac
     2821 atccagcgtc agaccctccg ccgctgcttt cggcggatat ggctccgact ccgtgtccga
     2881 atatgattcc agccaataca gcgtgaactc cgtggaggcc tgaccgcggg gatccgcccc
     2941 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     3001 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     3061 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     3121 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     3181 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     3241 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     3301 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     3361 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     3421 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     3481 gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtga gcaagggcga
     3541 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     3601 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3661 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     3721 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     3781 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3841 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     3901 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaaaaccat
     3961 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     4021 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     4081 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     4141 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     4201 ctccaccggc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc
     4261 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     4321 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     4381 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4441 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa
     4501 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     4561 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     4621 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     4681 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     4741 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     4801 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     4861 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     4921 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4981 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5041 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     5101 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5161 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5221 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5281 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5341 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     5401 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     5461 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     5521 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     5581 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     5641 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     5701 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     5761 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     5821 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     5881 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     5941 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     6001 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     6061 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     6121 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     6181 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     6241 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     6301 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6361 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6421 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     6481 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     6541 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     6601 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     6661 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     6721 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6781 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6841 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6901 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6961 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     7021 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7081 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7141 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7201 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7261 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7321 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7381 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7441 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7501 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7561 gccatgcat
//
