LOCUS pHAGE2_TetOminiC 7576 bp ds-DNA circular 20-NOV-2019 SOURCE ORGANISM COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers LTR 1..636 /gene="LTR" /vntifkey="19" /note="Long Terminal Repeat" /locus_tag="5'\LTR" /label="5'\LTR" /ApEinfo_label="5'\LTR" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 637..1155 /gene="psi" /product="pbs-gag" /vntifkey="21" /note="PSI Packaging Sequence" /locus_tag="PSI" /label="PSI" /ApEinfo_label="PSI" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1303..1536 /gene="rre" /product="minimal RRE" /vntifkey="21" /note="Rev Responsive Element" /locus_tag="RRE" /label="RRE" /ApEinfo_label="RRE" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2034..2211 /gene="cppt" /vntifkey="21" /note="Central Poly Purine Track" /locus_tag="HIV\cpPu\(Trip)" /label="HIV\cpPu\(Trip)" /ApEinfo_label="HIV\cpPu\(Trip)" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4017..4608 /gene="WPRE" /vntifkey="21" /note="Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element" /locus_tag="WPRE" /label="WPRE" /ApEinfo_label="WPRE" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 5190..6029 /gene="AMPr" /product="beta-lactamase (mature form)" /vntifkey="4" /note="Ampicillin Resistance Gene" /locus_tag="AMPr" /label="AMPr" /ApEinfo_label="AMPr" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 6258..6791 /gene="ORI" /product="ColE1 origin of replication" /vntifkey="33" /note="Bacterial Origin of Replication" /locus_tag="ORI" /label="ORI" /ApEinfo_label="ORI" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 7028..7151 /gene="SV40 ori" /vntifkey="21" /locus_tag="SV40\ori" /label="SV40\ori" /ApEinfo_label="SV40\ori" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 7471..7499 /vntifkey="28" /locus_tag="pHAGE-S1-500" /label="pHAGE-S1-500" /ApEinfo_label="pHAGE-S1-500" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 401..421 /vntifkey="28" /locus_tag="pHAGE-S501-1000" /label="pHAGE-S501-1000" /ApEinfo_label="pHAGE-S501-1000" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 898..919 /vntifkey="28" /locus_tag="pHAGE-S1001-1500" /label="pHAGE-S1001-1500" /ApEinfo_label="pHAGE-S1001-1500" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 1391..1417 /vntifkey="28" /locus_tag="pHAGE-S1501-2000" /label="pHAGE-S1501-2000" /ApEinfo_label="pHAGE-S1501-2000" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 1898..1922 /vntifkey="28" /locus_tag="pHAGE-S2001-2500" /label="pHAGE-S2001-2500" /ApEinfo_label="pHAGE-S2001-2500" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 4276..4296 /vntifkey="28" /locus_tag="pHAGE-S4001-4500" /label="pHAGE-S4001-4500" /ApEinfo_label="pHAGE-S4001-4500" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 4966..4988 /vntifkey="28" /locus_tag="pHAGE-S6001-6500" /label="pHAGE-S6001-6500" /ApEinfo_label="pHAGE-S6001-6500" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 5439..5463 /vntifkey="28" /locus_tag="pHAGE-S6501-7000" /label="pHAGE-S6501-7000" /ApEinfo_label="pHAGE-S6501-7000" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 5959..5983 /vntifkey="28" /locus_tag="pHAGE-S7001-7500" /label="pHAGE-S7001-7500" /ApEinfo_label="pHAGE-S7001-7500" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 6463..6487 /vntifkey="28" /locus_tag="pHAGE-S7501-8000" /label="pHAGE-S7501-8000" /ApEinfo_label="pHAGE-S7501-8000" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 6966..6985 /vntifkey="28" /locus_tag="pHAGE-S8001-8500" /label="pHAGE-S8001-8500" /ApEinfo_label="pHAGE-S8001-8500" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(158..179) /vntifkey="28" /locus_tag="pHAGE-A0050-8050" /label="pHAGE-A0050-8050" /ApEinfo_label="pHAGE-A0050-8050" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(667..690) /vntifkey="28" /locus_tag="pHAGE-A0550-0050" /label="pHAGE-A0550-0050" /ApEinfo_label="pHAGE-A0550-0050" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(1166..1189) /vntifkey="28" /locus_tag="pHAGE-A1050-0550" /label="pHAGE-A1050-0550" /ApEinfo_label="pHAGE-A1050-0550" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(1650..1678) /vntifkey="28" /locus_tag="pHAGE-A1550-1050" /label="pHAGE-A1550-1050" /ApEinfo_label="pHAGE-A1550-1050" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(2084..2112) /vntifkey="28" /locus_tag="pHAGE-A2050-1550" /label="pHAGE-A2050-1550" /ApEinfo_label="pHAGE-A2050-1550" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(4123..4146) /vntifkey="28" /locus_tag="pHAGE-A3550-3050" /label="pHAGE-A3550-3050" /ApEinfo_label="pHAGE-A3550-3050" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(5239..5262) /vntifkey="28" /locus_tag="pHAGE-A6050-5550" /label="pHAGE-A6050-5550" /ApEinfo_label="pHAGE-A6050-5550" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(5708..5731) /vntifkey="28" /locus_tag="pHAGE-A6550-6050" /label="pHAGE-A6550-6050" /ApEinfo_label="pHAGE-A6550-6050" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(6219..6240) /vntifkey="28" /locus_tag="pHAGE-A7050-6550" /label="pHAGE-A7050-6550" /ApEinfo_label="pHAGE-A7050-6550" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(6730..6751) /vntifkey="28" /locus_tag="pHAGE-A7550-7050" /label="pHAGE-A7550-7050" /ApEinfo_label="pHAGE-A7550-7050" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(7206..7232) /vntifkey="28" /locus_tag="pHAGE-A8050-7550" /label="pHAGE-A8050-7550" /ApEinfo_label="pHAGE-A8050-7550" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 455..551 /vntifkey="21" /note="HIV Repeat Region" /locus_tag="HIV\R" /label="HIV\R" /ApEinfo_label="HIV\R" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 636..658 /vntifkey="21" /note="tRNA binding site for Lysine tRNA" /locus_tag="PBS-K" /label="PBS-K" /ApEinfo_label="PBS-K" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 697..806 /vntifkey="21" /locus_tag="HIV-PSI" /label="HIV-PSI" /ApEinfo_label="HIV-PSI" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 807..1144 /vntifkey="21" /note="HIV gag sequence 351bp to 688bp" /locus_tag="HR-ePSI" /label="HR-ePSI" /ApEinfo_label="HR-ePSI" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 552..636 /vntifkey="21" /note="HIV U5" /locus_tag="HIV\U5" /label="HIV\U5" /ApEinfo_label="HIV\U5" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1..454 /vntifkey="21" /note="Full Length HIV U3" /locus_tag="HIV\U3" /label="HIV\U3" /ApEinfo_label="HIV\U3" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 5137..5171 /vntifkey="30" /note="Beta Lactamase Promoter" /locus_tag="P-Bla" /label="P-Bla" /ApEinfo_label="P-Bla" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(4045..4079) /vntifkey="28" /locus_tag="pHAGE\3'\CDS" /label="pHAGE\3'\CDS" /ApEinfo_label="pHAGE\3'\CDS" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4613..4714 /vntifkey="21" /note="HIV nef" /locus_tag="HIV\nef" /label="HIV\nef" /ApEinfo_label="HIV\nef" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4755..4851 /vntifkey="21" /locus_tag="HIV\R(1)" /label="HIV\R(1)" /ApEinfo_label="HIV\R" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4852..4934 /vntifkey="21" /note="HIV U5" /locus_tag="HIV\U5(1)" /label="HIV\U5(1)" /ApEinfo_label="HIV\U5" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4663..4677 /vntifkey="21" /note="PolyPurine Tract" /locus_tag="HIV\PPT" /label="HIV\PPT" /ApEinfo_label="HIV\PPT" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" mutation 833..834 /vntifkey="62" /note="Filled in Cla Site from original HIV genome to disrupt gag polyprotein ORF" /locus_tag="Fill-in" /label="Fill-in" /ApEinfo_label="Fill-in" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" LTR 4613..4934 /vntifkey="19" /locus_tag="3'\LTR" /label="3'\LTR" /ApEinfo_label="3'\LTR" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" splicing_signal 744..745 /vntifkey="38" /note="Major 5' Splice Donor 289" /locus_tag="5'\SD\289" /label="5'\SD\289" /ApEinfo_label="5'\SD\289" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" splicing_signal 1911..1912 /vntifkey="38" /locus_tag="SA\7925" /label="SA\7925" /ApEinfo_label="SA\7925" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" splicing_signal 2188..2189 /vntifkey="38" /locus_tag="SA\4459" /label="SA\4459" /ApEinfo_label="SA\4459" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" mutation 1357..1357 /vntifkey="62" /note="This G is a C in the WT HIV-1 genome" /locus_tag="RRE\Mutation" /label="RRE\Mutation" /ApEinfo_label="RRE\Mutation" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2014..2027 /vntifkey="21" /note="Linker Junk Sequence probably left over from cloning" /locus_tag="Junk" /label="Junk" /ApEinfo_label="Junk" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 1156..2013 /vntifkey="4" /note="HIV env sequence from bp7168 to 8025 (contains RRE) Cloned from BglII to BamHI out of HIV" /locus_tag="env" /label="env" /ApEinfo_label="env" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 790..1144 /vntifkey="4" /note="HIV gag sequence from 336bp to 688bp (Clone to PvuII site in HIV and filled in ClaI digest to take gag CDS out of frame)" /locus_tag="HIV\gag" /label="HIV\gag" /ApEinfo_label="HIV\gag" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" 5'UTR 551..635 /vntifkey="52" /note="HIV 5' UTR" /locus_tag="5'\UTR" /label="5'\UTR" /ApEinfo_label="5'\UTR" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 853..855 /vntifkey="21" /note="Stop codon in gag put in frame by ClaI fill in" /locus_tag="Stop\Codon" /label="Stop\Codon" /ApEinfo_label="Stop\Codon" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1245..1593 /vntifkey="21" /note="Rev Responsive Element according to Belasco in Molecular Cell, Vol. 7, 603-614, March, 2001" /locus_tag="RRE(1)" /label="RRE(1)" /ApEinfo_label="RRE" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4680..4716 /vntifkey="21" /note="1st Half of deleted U3" /locus_tag="dU3" /label="dU3" /ApEinfo_label="dU3" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4736..4754 /vntifkey="21" /note="2nd Half of deleted U3" /locus_tag="dU3(1)" /label="dU3(1)" /ApEinfo_label="dU3" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" stem_loop 697..731 /vntifkey="39" /note="PSI Stem loop 1" /locus_tag="SL-1" /label="SL-1" /ApEinfo_label="SL-1" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" stem_loop 736..754 /vntifkey="39" /note="PSI Stem loop 2" /locus_tag="SL-2" /label="SL-2" /ApEinfo_label="SL-2" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" stem_loop 766..779 /vntifkey="39" /note="PSI Stem Loop 3" /locus_tag="SL-3" /label="SL-3" /ApEinfo_label="SL-3" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" stem_loop 793..806 /vntifkey="39" /note="PSI Stem Loop 4" /locus_tag="SL-4" /label="SL-4" /ApEinfo_label="SL-4" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_site 550..550 /vntifkey="26" /note="Exact base to which Poly-A is added" /locus_tag="pA\Site" /label="pA\Site" /ApEinfo_label="pA\Site" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal 570..577 /vntifkey="25" /note="GT Cluster shown to be necessary for Poly-A site activity by Cullen in J Virol. 1989 January; 63(1):421-424" /locus_tag="G-T\cluster" /label="G-T\cluster" /ApEinfo_label="G-T\cluster" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 2556..2675 /vntifkey="29" /locus_tag="miniCMV" /label="miniCMV" /ApEinfo_label="miniCMV" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2238..2555 /vntifkey="21" /note="Tetracyclin responsive element (TRE)" /locus_tag="TetO" /label="TetO" /ApEinfo_label="TetO" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 6127..6809 /locus_tag="ColE1 origin" /label="ColE1 origin" /ApEinfo_label="ColE1 origin" /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_binding 7272..7294 /locus_tag="LacO" /label="LacO" /ApEinfo_label="LacO" /ApEinfo_fwdcolor="#6495ed" /ApEinfo_revcolor="#6495ed" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 5370..6029 /locus_tag="AmpR" /label="AmpR" /ApEinfo_label="AmpR" /ApEinfo_fwdcolor="yellow" /ApEinfo_revcolor="yellow" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4321..4400 /locus_tag="WPRE(1)" /label="WPRE(1)" /ApEinfo_label="WPRE" /ApEinfo_fwdcolor="#a5c4b3" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2062..2076 /locus_tag="cPPT" /label="cPPT" /ApEinfo_label="cPPT" /ApEinfo_fwdcolor="#a596b4" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4663..4677 /locus_tag="cPPT(1)" /label="cPPT(1)" /ApEinfo_label="cPPT" /ApEinfo_fwdcolor="#a596b4" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2685..4001 /locus_tag="mC-Myc" /label="mC-Myc" /ApEinfo_label="mC-Myc" /ApEinfo_fwdcolor="#4c4c4c" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca 2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt 2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta 2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt 2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac 2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag 2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat 2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatgccc ctcaacgtga 2701 acttcaccaa caggaactat gacctcgact acgactccgt acagccctat ttcatctgcg 2761 acgaggaaga gaatttctat caccagcaac agcagagcga gctgcagccg cccgcgccca 2821 gtgaggatat ctggaagaaa ttcgagctgc ttcccacccc gcccctgtcc ccgagccgcc 2881 gctccgggct ctgctctcca tcctatgttg cggtcgctac gtccttctcc ccaagggaag 2941 acgatgacgg cggcggtggc aacttctcca ccgccgatca gctggagatg atgaccgagt 3001 tacttggagg agacatggtg aaccagagct tcatctgcga tcctgacgac gagaccttca 3061 tcaagaacat catcatccag gactgtatgt ggagcggttt ctcagccgct gccaagctgg 3121 tctcggagaa gctggcctcc taccaggctg cgcgcaaaga cagcaccagc ctgagccccg 3181 cccgcgggca cagcgtctgc tccacctcca gcctgtacct gcaggacctc accgccgccg 3241 cgtccgagtg cattgacccc tcagtggtct ttccctaccc gctcaacgac agcagctcgc 3301 ccaaatcctg tacctcgtcc gattccacgg ccttctctcc ttcctcggac tcgctgctgt 3361 cctccgagtc ctccccacgg gccagccctg agcccctagt gctgcatgag gagacaccgc 3421 ccaccaccag cagcgactct gaagaagagc aagaagatga ggaagaaatt gatgtggtgt 3481 ctgtggagaa gaggcaaacc cctgccaaga ggtcggagtc gggctcatct ccatcccgag 3541 gccacagcaa acctccgcac agcccactgg tcctcaagag gtgccacgtc tccactcacc 3601 agcacaacta cgccgcaccc ccctccacaa ggaaggacta tccagctgcc aagagggcca 3661 agttggacag tggcagggtc ctgaagcaga tcagcaacaa ccgcaagtgc tccagcccca 3721 ggtcctcaga cacggaggaa aacgacaaga ggcggacaca caacgtcttg gaacgtcaga 3781 ggaggaacga gctgaagcgc agcttttttg ccctgcgtga ccagatccct gaattggaaa 3841 acaacgaaaa ggcccccaag gtagtgatcc tcaaaaaagc caccgcctac atcctgtcca 3901 ttcaagcaga cgagcacaag ctcacctctg aaaaggactt attgaggaaa cgacgagaac 3961 agttgaaaca caaactcgaa cagcttcgaa actctggtgc ataaatcgat agatcctaat 4021 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 4081 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 4141 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 4201 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 4261 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 4321 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 4381 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc 4441 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 4501 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 4561 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag 4621 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 4681 ggaagggcta attcactccc aacgaagaca agatcacctg caggacaggc gcgccctgct 4741 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 4801 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 4861 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 4921 gaaaatctct agcacccggg cgattaagga aagggctaga tcattcttga agacgaaagg 4981 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt 5041 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 5101 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 5161 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 5221 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 5281 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 5341 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 5401 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc 5461 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 5521 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 5581 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 5641 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 5701 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 5761 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 5821 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 5881 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 5941 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 6001 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 6061 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 6121 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 6181 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 6241 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 6301 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 6361 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 6421 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 6481 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 6541 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 6601 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 6661 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 6721 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 6781 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 6841 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 6901 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 6961 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 7021 aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 7081 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 7141 aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 7201 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 7261 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga 7321 ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 7381 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc 7441 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct 7501 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta 7561 ctacaaactt agtagt //