LOCUS       pHAGE2_TetOminiC        6967 bp ds-DNA     circular     04-JUL-2018
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     LTR             1..636
                     /gene="LTR"
                     /vntifkey="19"
                     /note="Long Terminal Repeat"
                     /locus_tag="5'\LTR"
                     /label="5'\LTR"
                     /ApEinfo_label="5'\LTR"
                     /ApEinfo_fwdcolor="#86f71d"
                     /ApEinfo_revcolor="#d7336f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    637..1155
                     /gene="psi"
                     /product="pbs-gag"
                     /vntifkey="21"
                     /note="PSI Packaging Sequence"
                     /locus_tag="PSI"
                     /label="PSI"
                     /ApEinfo_label="PSI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1303..1536
                     /gene="rre"
                     /product="minimal RRE"
                     /vntifkey="21"
                     /note="Rev Responsive Element"
                     /locus_tag="RRE"
                     /label="RRE"
                     /ApEinfo_label="RRE"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2034..2211
                     /gene="cppt"
                     /vntifkey="21"
                     /note="Central Poly Purine Track"
                     /locus_tag="HIV\cpPu\(Trip)"
                     /label="HIV\cpPu\(Trip)"
                     /ApEinfo_label="HIV\cpPu\(Trip)"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3408..3999
                     /gene="WPRE"
                     /vntifkey="21"
                     /note="Woodchuck Hepatitis Virus Posttranscriptional
                     Regulatory Element"
                     /locus_tag="WPRE"
                     /label="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             4581..5420
                     /gene="AMPr"
                     /product="beta-lactamase (mature form)"
                     /vntifkey="4"
                     /note="Ampicillin Resistance Gene"
                     /locus_tag="AMPr"
                     /label="AMPr"
                     /ApEinfo_label="AMPr"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      5649..6182
                     /gene="ORI"
                     /product="ColE1 origin of replication"
                     /vntifkey="33"
                     /note="Bacterial Origin of Replication"
                     /locus_tag="ORI"
                     /label="ORI"
                     /ApEinfo_label="ORI"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6419..6542
                     /gene="SV40 ori"
                     /vntifkey="21"
                     /locus_tag="SV40\ori"
                     /label="SV40\ori"
                     /ApEinfo_label="SV40\ori"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     6862..6890
                     /vntifkey="28"
                     /locus_tag="pHAGE-S1-500"
                     /label="pHAGE-S1-500"
                     /ApEinfo_label="pHAGE-S1-500"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     401..421
                     /vntifkey="28"
                     /locus_tag="pHAGE-S501-1000"
                     /label="pHAGE-S501-1000"
                     /ApEinfo_label="pHAGE-S501-1000"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     898..919
                     /vntifkey="28"
                     /locus_tag="pHAGE-S1001-1500"
                     /label="pHAGE-S1001-1500"
                     /ApEinfo_label="pHAGE-S1001-1500"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1391..1417
                     /vntifkey="28"
                     /locus_tag="pHAGE-S1501-2000"
                     /label="pHAGE-S1501-2000"
                     /ApEinfo_label="pHAGE-S1501-2000"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1898..1922
                     /vntifkey="28"
                     /locus_tag="pHAGE-S2001-2500"
                     /label="pHAGE-S2001-2500"
                     /ApEinfo_label="pHAGE-S2001-2500"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3667..3687
                     /vntifkey="28"
                     /locus_tag="pHAGE-S4001-4500"
                     /label="pHAGE-S4001-4500"
                     /ApEinfo_label="pHAGE-S4001-4500"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4357..4379
                     /vntifkey="28"
                     /locus_tag="pHAGE-S6001-6500"
                     /label="pHAGE-S6001-6500"
                     /ApEinfo_label="pHAGE-S6001-6500"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4830..4854
                     /vntifkey="28"
                     /locus_tag="pHAGE-S6501-7000"
                     /label="pHAGE-S6501-7000"
                     /ApEinfo_label="pHAGE-S6501-7000"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     5350..5374
                     /vntifkey="28"
                     /locus_tag="pHAGE-S7001-7500"
                     /label="pHAGE-S7001-7500"
                     /ApEinfo_label="pHAGE-S7001-7500"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     5854..5878
                     /vntifkey="28"
                     /locus_tag="pHAGE-S7501-8000"
                     /label="pHAGE-S7501-8000"
                     /ApEinfo_label="pHAGE-S7501-8000"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     6357..6376
                     /vntifkey="28"
                     /locus_tag="pHAGE-S8001-8500"
                     /label="pHAGE-S8001-8500"
                     /ApEinfo_label="pHAGE-S8001-8500"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(158..179)
                     /vntifkey="28"
                     /locus_tag="pHAGE-A0050-8050"
                     /label="pHAGE-A0050-8050"
                     /ApEinfo_label="pHAGE-A0050-8050"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(667..690)
                     /vntifkey="28"
                     /locus_tag="pHAGE-A0550-0050"
                     /label="pHAGE-A0550-0050"
                     /ApEinfo_label="pHAGE-A0550-0050"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1166..1189)
                     /vntifkey="28"
                     /locus_tag="pHAGE-A1050-0550"
                     /label="pHAGE-A1050-0550"
                     /ApEinfo_label="pHAGE-A1050-0550"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1650..1678)
                     /vntifkey="28"
                     /locus_tag="pHAGE-A1550-1050"
                     /label="pHAGE-A1550-1050"
                     /ApEinfo_label="pHAGE-A1550-1050"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2084..2112)
                     /vntifkey="28"
                     /locus_tag="pHAGE-A2050-1550"
                     /label="pHAGE-A2050-1550"
                     /ApEinfo_label="pHAGE-A2050-1550"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3514..3537)
                     /vntifkey="28"
                     /locus_tag="pHAGE-A3550-3050"
                     /label="pHAGE-A3550-3050"
                     /ApEinfo_label="pHAGE-A3550-3050"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4630..4653)
                     /vntifkey="28"
                     /locus_tag="pHAGE-A6050-5550"
                     /label="pHAGE-A6050-5550"
                     /ApEinfo_label="pHAGE-A6050-5550"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5099..5122)
                     /vntifkey="28"
                     /locus_tag="pHAGE-A6550-6050"
                     /label="pHAGE-A6550-6050"
                     /ApEinfo_label="pHAGE-A6550-6050"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5610..5631)
                     /vntifkey="28"
                     /locus_tag="pHAGE-A7050-6550"
                     /label="pHAGE-A7050-6550"
                     /ApEinfo_label="pHAGE-A7050-6550"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6121..6142)
                     /vntifkey="28"
                     /locus_tag="pHAGE-A7550-7050"
                     /label="pHAGE-A7550-7050"
                     /ApEinfo_label="pHAGE-A7550-7050"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6597..6623)
                     /vntifkey="28"
                     /locus_tag="pHAGE-A8050-7550"
                     /label="pHAGE-A8050-7550"
                     /ApEinfo_label="pHAGE-A8050-7550"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    455..551
                     /vntifkey="21"
                     /note="HIV Repeat Region"
                     /locus_tag="HIV\R"
                     /label="HIV\R"
                     /ApEinfo_label="HIV\R"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    636..658
                     /vntifkey="21"
                     /note="tRNA binding site for Lysine tRNA"
                     /locus_tag="PBS-K"
                     /label="PBS-K"
                     /ApEinfo_label="PBS-K"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    697..806
                     /vntifkey="21"
                     /locus_tag="HIV-PSI"
                     /label="HIV-PSI"
                     /ApEinfo_label="HIV-PSI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    807..1144
                     /vntifkey="21"
                     /note="HIV gag sequence 351bp to 688bp"
                     /locus_tag="HR-ePSI"
                     /label="HR-ePSI"
                     /ApEinfo_label="HR-ePSI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    552..636
                     /vntifkey="21"
                     /note="HIV U5"
                     /locus_tag="HIV\U5"
                     /label="HIV\U5"
                     /ApEinfo_label="HIV\U5"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1..454
                     /vntifkey="21"
                     /note="Full Length HIV U3"
                     /locus_tag="HIV\U3"
                     /label="HIV\U3"
                     /ApEinfo_label="HIV\U3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        4528..4562
                     /vntifkey="30"
                     /note="Beta Lactamase Promoter"
                     /locus_tag="P-Bla"
                     /label="P-Bla"
                     /ApEinfo_label="P-Bla"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3436..3470)
                     /vntifkey="28"
                     /locus_tag="pHAGE\3'\CDS"
                     /label="pHAGE\3'\CDS"
                     /ApEinfo_label="pHAGE\3'\CDS"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4004..4105
                     /vntifkey="21"
                     /note="HIV nef"
                     /locus_tag="HIV\nef"
                     /label="HIV\nef"
                     /ApEinfo_label="HIV\nef"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4146..4242
                     /vntifkey="21"
                     /locus_tag="HIV\R(1)"
                     /label="HIV\R(1)"
                     /ApEinfo_label="HIV\R"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4243..4325
                     /vntifkey="21"
                     /note="HIV U5"
                     /locus_tag="HIV\U5(1)"
                     /label="HIV\U5(1)"
                     /ApEinfo_label="HIV\U5"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4054..4068
                     /vntifkey="21"
                     /note="PolyPurine Tract"
                     /locus_tag="HIV\PPT"
                     /label="HIV\PPT"
                     /ApEinfo_label="HIV\PPT"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mutation        833..834
                     /vntifkey="62"
                     /note="Filled in Cla Site from original HIV genome to
                     disrupt gag polyprotein ORF"
                     /locus_tag="Fill-in"
                     /label="Fill-in"
                     /ApEinfo_label="Fill-in"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     LTR             4004..4325
                     /vntifkey="19"
                     /locus_tag="3'\LTR"
                     /label="3'\LTR"
                     /ApEinfo_label="3'\LTR"
                     /ApEinfo_fwdcolor="#86f71d"
                     /ApEinfo_revcolor="#d7336f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     splicing_signal 744..745
                     /vntifkey="38"
                     /note="Major 5' Splice Donor 289"
                     /locus_tag="5'\SD\289"
                     /label="5'\SD\289"
                     /ApEinfo_label="5'\SD\289"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     splicing_signal 1911..1912
                     /vntifkey="38"
                     /locus_tag="SA\7925"
                     /label="SA\7925"
                     /ApEinfo_label="SA\7925"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     splicing_signal 2188..2189
                     /vntifkey="38"
                     /locus_tag="SA\4459"
                     /label="SA\4459"
                     /ApEinfo_label="SA\4459"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mutation        1357..1357
                     /vntifkey="62"
                     /note="This G is a C in the WT HIV-1 genome"
                     /locus_tag="RRE\Mutation"
                     /label="RRE\Mutation"
                     /ApEinfo_label="RRE\Mutation"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2014..2027
                     /vntifkey="21"
                     /note="Linker Junk Sequence probably left over from
                     cloning"
                     /locus_tag="Junk"
                     /label="Junk"
                     /ApEinfo_label="Junk"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1156..2013
                     /vntifkey="4"
                     /note="HIV env sequence from bp7168 to 8025 (contains RRE)
                     Cloned from BglII to BamHI out of HIV"
                     /locus_tag="env"
                     /label="env"
                     /ApEinfo_label="env"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             790..1144
                     /vntifkey="4"
                     /note="HIV gag sequence from 336bp to 688bp (Clone to
                     PvuII site in HIV and filled in ClaI digest to take gag
                     CDS out of frame)"
                     /locus_tag="HIV\gag"
                     /label="HIV\gag"
                     /ApEinfo_label="HIV\gag"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     5'UTR           551..635
                     /vntifkey="52"
                     /note="HIV 5' UTR"
                     /locus_tag="5'\UTR"
                     /label="5'\UTR"
                     /ApEinfo_label="5'\UTR"
                     /ApEinfo_fwdcolor="#ffd327"
                     /ApEinfo_revcolor="#ffd327"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    853..855
                     /vntifkey="21"
                     /note="Stop codon in gag put in frame by ClaI fill in"
                     /locus_tag="Stop\Codon"
                     /label="Stop\Codon"
                     /ApEinfo_label="Stop\Codon"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1245..1593
                     /vntifkey="21"
                     /note="Rev Responsive Element according to Belasco in
                     Molecular Cell, Vol. 7, 603-614, March, 2001"
                     /locus_tag="RRE(1)"
                     /label="RRE(1)"
                     /ApEinfo_label="RRE"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4071..4107
                     /vntifkey="21"
                     /note="1st Half of deleted U3"
                     /locus_tag="dU3"
                     /label="dU3"
                     /ApEinfo_label="dU3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4127..4145
                     /vntifkey="21"
                     /note="2nd Half of deleted U3"
                     /locus_tag="dU3(1)"
                     /label="dU3(1)"
                     /ApEinfo_label="dU3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     stem_loop       697..731
                     /vntifkey="39"
                     /note="PSI Stem loop 1"
                     /locus_tag="SL-1"
                     /label="SL-1"
                     /ApEinfo_label="SL-1"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     stem_loop       736..754
                     /vntifkey="39"
                     /note="PSI Stem loop 2"
                     /locus_tag="SL-2"
                     /label="SL-2"
                     /ApEinfo_label="SL-2"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     stem_loop       766..779
                     /vntifkey="39"
                     /note="PSI Stem Loop 3"
                     /locus_tag="SL-3"
                     /label="SL-3"
                     /ApEinfo_label="SL-3"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     stem_loop       793..806
                     /vntifkey="39"
                     /note="PSI Stem Loop 4"
                     /locus_tag="SL-4"
                     /label="SL-4"
                     /ApEinfo_label="SL-4"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_site      550..550
                     /vntifkey="26"
                     /note="Exact base to which Poly-A is added"
                     /locus_tag="pA\Site"
                     /label="pA\Site"
                     /ApEinfo_label="pA\Site"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    570..577
                     /vntifkey="25"
                     /note="GT Cluster shown to be necessary for Poly-A site
                     activity by Cullen in J Virol. 1989 January;
                     63(1):421-424"
                     /locus_tag="G-T\cluster"
                     /label="G-T\cluster"
                     /ApEinfo_label="G-T\cluster"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2556..2675
                     /vntifkey="29"
                     /locus_tag="miniCMV"
                     /label="miniCMV"
                     /ApEinfo_label="miniCMV"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2238..2555
                     /vntifkey="21"
                     /note="Tetracyclin responsive element (TRE)"
                     /locus_tag="TetO"
                     /label="TetO"
                     /ApEinfo_label="TetO"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2685..3373
                     /locus_tag="mCherry"
                     /label="mCherry"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3374..3395
                     /locus_tag="mCherry(1)"
                     /label="mCherry(1)"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat
     2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccATGGTG AGCAAGGGCG
     2701 AGGAGGATAA CATGGCCATC ATCAAGGAGT TCATGCGCTT CAAGGTGCAC ATGGAGGGCT
     2761 CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCA
     2821 CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT GCCCTTCGCC TGGGACATCC
     2881 TGTCCCCTCA GTTCATGTAC GGCTCCAAGG CCTACGTGAA GCACCCCGCC GACATCCCCG
     2941 ACTACTTGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG
     3001 ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCGAG TTCATCTACA
     3061 AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC CGTAATGCAG AAGAAGACCA
     3121 TGGGCTGGGA GGCCTCCTCC GAGCGGATGT ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA
     3181 TCAAGCAGAG GCTGAAGCTG AAGGACGGCG GCCACTACGA CGCTGAGGTC AAGACCACCT
     3241 ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAA CGTCAACATC AAGTTGGACA
     3301 TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACAGTA CGAACGCGCC GAGGGCCGCC
     3361 ACTCCACCGG CGGCATGGAC GAGCTGTACA AGTAAatcga tagatcctaa tcaacctctg
     3421 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3481 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3541 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3601 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3661 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3721 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3781 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     3841 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3901 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3961 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac
     4021 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     4081 aattcactcc caacgaagac aagatcacct gcaggacagg cgcgccctgc tttttgcttg
     4141 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     4201 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     4261 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     4321 tagcacccgg gcgattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga
     4381 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     4441 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4501 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4561 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4621 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4681 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4741 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4801 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4861 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4921 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4981 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5041 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5101 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5161 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5221 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5281 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5341 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5401 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5461 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5521 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5581 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5641 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5701 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5761 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5821 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5881 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5941 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6001 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6061 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6121 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6181 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6241 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6301 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6361 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca
     6421 agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6481 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     6541 cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     6601 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     6661 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt
     6721 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6781 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     6841 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     6901 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     6961 tagtagt     
//
