LOCUS pCDNA3.0_nucLAMA 8272 bp DNA circular UNA 08-NOV-2019 DEFINITION NLS001_SNAP-LAMA-F98-NLS IRES EGFP pcDNA3.0. ACCESSION urn.local...3x-b7nwlne VERSION urn.local...3x-b7nwlne KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 236..852 /standard_name="CMV_immearly_promoter" enhancer 315..602 /note="Geneious type: Enhancer" /standard_name="CAG_enhancer" promoter 863..881 /standard_name="T7 Promotor" misc_feature 890..897 /note="Geneious type: Editing History Replacement" /standard_name="AGCTT" misc_feature 898..900 /note="Geneious type: Misc_Feat" /standard_name="kozak" CDS 901..1446 /standard_name="SNAP" misc_feature 948 /note="Geneious type: Editing History Replacement" /standard_name="G" CDS 1453..1764 /standard_name="VHH_Enhancer" gene 1765..2256 /note="Geneious type: Gene" /standard_name="cpDHFRL24G5" misc_feature 2232 /note="Geneious type: Editing History Replacement" /standard_name="C" CDS 2257..2295 /standard_name="VHH_Enhancer" misc_feature 2296..2409 /note="Geneious type: Misc_Feat\" /standard_name="NLS" misc_feature 2299..2409 /note="Geneious type: Editing History Replacement" /standard_name="CTGAACCCTCCTGATGAGAGTGGCCCCGGCTGCATGAGCTGC AAGTGTGTGCTCTCC" misc_feature 2473..3045 /note="Geneious type: Misc_Feat" /standard_name="EMCV IRES" misc_feature 3046..3793 /note="Geneious type: Editing History Replacement" /standard_name="ATGGCTAGCAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCA ATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGG GTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAA ACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCTCTTATGGTGTTCAATGCTTT TCAAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAG GTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGC TGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGAT TTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACA ATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAG ACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCA ATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCC TTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGC TGCTGGGATTACACATGGCATGGATGAACTATACAAA" CDS 3047..3793 /standard_name="EGFP" promoter complement(3826..3843) /standard_name="SP6 promotor" terminator 3865..4092 /standard_name="bGH_PA_terminator" polyA_site 3870..4059 rep_origin 4155..4461 enhancer complement(4581..4797) /note="Geneious type: Enhancer" /standard_name="SV40_enhancer" promoter 4593..4862 /standard_name="SV40_promoter" rep_origin complement(4761..4838) CDS 4980..5768 /standard_name="NeoR/KanR" terminator 5948..6067 /standard_name="SV40_PA_terminator" promoter complement(6164..6193) /standard_name="lac_promoter" rep_origin complement(6502..6821) CDS complement(7296..8136) /standard_name="Amp" promoter complement(8178..8206) /standard_name="AmpR Promotor" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccag cggccgcacc 901 atggataaag actgcgaaat gaaacgcact accctggaca gcccgcttgg taaactggaa 961 ctgagcggtt gcgagcaggg cctgcacgaa atcattttcc tgggcaaagg tacgagcgct 1021 gcggatgcgg tcgaagtacc ggctccggct gcggttctgg gtggcccgga gccgctgatg 1081 caggctaccg catggctgaa cgcatatttt caccagccag aggcgattga agagttccct 1141 gtaccggccc tgcatcaccc ggtatttcag caggaatcct ttacccgtca ggtactgtgg 1201 aaactgctga aagtagtgaa attcggcgag gtgatctcct attcccatct ggcggcgctg 1261 gctggcaacc cagcggcaac tgcggcggtg aaaaccgctc tgtctggtaa tccggtgcca 1321 attctgatcc cgtgccaccg tgtggttcag ggcgacctgg acgtaggtgg ctacgaaggc 1381 ggcctggctg tgaaagaatg gctgctggcg cacgaaggtc accgtctggg taaaccaggc 1441 ctgggcggcc gcatggccca ggtgcagctg gttgagagcg gcggtgctct ggtgcagccg 1501 ggcggtagcc tgcgtctgag ctgcgccgcg agcggcttcc cggtgaaccg ctacagcatg 1561 cgttggtacc gtcaggcgcc gggcaaggag cgtgaatggg tggctggtat gagcagcgcc 1621 ggtgaccgta gcagctacga agatagcgtg aaaggccgct tcaccatcag ccgtgacgat 1681 gcccgcaaca ccgtgtatct gcagatgaac agcctgaagc cggaggacac cgcggtgtac 1741 tattgcaacg tgaacgtggg ttttctgcct gccgatctcg cctggtttaa acgcaacacc 1801 ttaaataaac ccgtgattat gggccgccat acctgggaat caatcggtcg tccgttgcca 1861 ggacgcaaaa atattatcct cagcagtcaa ccgggtacgg acgatcgcgt aacgtgggtg 1921 aagtcggtgg atgaagccat cgcggcgtgt ggtgacgtac cagaaatcat ggtgattggc 1981 ggcggtcgcg tttatgaaca gttcttgcca aaagcgcaaa aactgtatct gacgcatatc 2041 gacgcagaag tggaaggcga cacccatttc ccggattacg agccggatga ctgggaatcg 2101 gtattcagcg aattccacga tgctgatgcg cagaactctc acagctattg ctttgagatt 2161 ctggagcggc ggggcggcgg cggcggcatg atcagtctga ttgcggcgtt agcggtagat 2221 cgcgttatcg gaatggaaaa cgccatgccg tggaacgaat actggggcca gggtacccag 2281 gtgaccgtga gcagcaagtc cggactcaga tctcgagctg atccaaaaaa gaagagaaag 2341 gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt aggatccacc 2401 ggatctagat agtaaggatc cacccagctt tcttgtacaa agtggtgatc gcgtaaacta 2461 cgggctgcag gaattccgcc cccccccccc taacgttact ggccgaagcc gcttggaata 2521 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt 2581 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct 2641 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc 2701 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga 2761 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc 2821 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt 2881 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg 2941 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg 3001 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aataccatgg tgagcaaggg 3061 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 3121 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 3181 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 3241 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 3301 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 3361 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 3421 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 3481 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 3541 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 3601 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 3661 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 3721 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctcg 3781 actagctagt agctaactcg agcatgcatc tagagggccc tattctatag tgtcacctaa 3841 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt 3901 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 3961 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 4021 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 4081 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg 4141 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 4201 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 4261 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg 4321 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 4381 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 4441 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 4501 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 4561 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg 4621 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 4681 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 4741 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 4801 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 4861 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 4921 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga 4981 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 5041 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 5101 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg 5161 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 5221 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 5281 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 5341 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 5401 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 5461 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg 5521 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 5581 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 5641 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 5701 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 5761 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 5821 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 5881 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 5941 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 6001 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 6061 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 6121 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 6181 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 6241 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 6301 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 6361 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 6421 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 6481 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 6541 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 6601 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 6661 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg 6721 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 6781 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 6841 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 6901 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 6961 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7021 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 7081 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 7141 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 7201 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 7261 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 7321 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 7381 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 7441 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 7501 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 7561 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 7621 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 7681 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 7741 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 7801 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 7861 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 7921 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 7981 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 8041 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 8101 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 8161 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 8221 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc //