LOCUS pCDNA3.0_nucNano 7780 bp DNA circular UNA 07-NOV-2019 DEFINITION NLS003_SNAP-VHHEnhancer-NLS IRES EGFP pcDNA3.0. ACCESSION urn.local...47-b7nwlns VERSION urn.local...47-b7nwlns KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 236..852 /standard_name="CMV_immearly_promoter" enhancer 315..602 /note="Geneious type: Enhancer" /standard_name="CAG_enhancer" promoter 863..881 /standard_name="T7 Promotor" misc_difference 889 /standard_name="Addition of A" misc_feature 890..897 /note="Geneious type: Editing History Replacement" /standard_name="AGCTT" misc_feature 898..900 /note="Geneious type: Misc_Feat" /standard_name="kozak" CDS 901..1446 /standard_name="SNAP" misc_feature 948 /note="Geneious type: Editing History Replacement" /standard_name="G" CDS 1453..1803 /standard_name="VHH_Enhancer" misc_feature 1764^1765 /note="Geneious type: Editing History Deletion" /standard_name="CTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAAT AAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC GCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGGGTGAA GTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGC GGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATA TCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGA ATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTT GAGATTCTGGAGCGGCGGGGCGGCGGCGGCGGCATGATCAGTCTGATTGCGGCGTTAG CGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAC" misc_feature 1804..1917 /note="Geneious type: Misc_Feat\" /standard_name="NLS" misc_feature 1807..1917 /note="Geneious type: Editing History Replacement" /standard_name="CTGAACCCTCCTGATGAGAGTGGCCCCGGCTGCATGAGCTGC AAGTGTGTGCTCTCC" misc_feature complement(1933..1953) /note="Geneious type: attB2" /standard_name="attB2" misc_feature 1981..2553 /note="Geneious type: Misc_Feat" /standard_name="IRES" misc_feature 2554..3301 /note="Geneious type: Editing History Replacement" /standard_name="ATGGCTAGCAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCA ATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGG GTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAA ACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCTCTTATGGTGTTCAATGCTTT TCAAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAG GTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGC TGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGAT TTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACA ATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAG ACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCA ATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCC TTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGC TGCTGGGATTACACATGGCATGGATGAACTATACAAA" CDS 2555..3300 /standard_name="EGFP" promoter complement(3334..3351) /standard_name="SP6 promotor" terminator 3373..3600 /standard_name="bGH_PA_terminator" polyA_site 3378..3567 rep_origin 3663..3969 enhancer complement(4089..4305) /note="Geneious type: Enhancer" /standard_name="SV40_enhancer" promoter 4101..4370 /standard_name="SV40_promoter" rep_origin complement(4269..4346) CDS 4488..5276 /standard_name="NeoR/KanR" terminator 5456..5575 /standard_name="SV40_PA_terminator" promoter complement(5672..5701) /standard_name="lac_promoter" rep_origin complement(6010..6329) CDS complement(6804..7644) /standard_name="Amp" promoter complement(7686..7714) /standard_name="AmpR Promotor" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccag cggccgcacc 901 atggataaag actgcgaaat gaaacgcact accctggaca gcccgcttgg taaactggaa 961 ctgagcggtt gcgagcaggg cctgcacgaa atcattttcc tgggcaaagg tacgagcgct 1021 gcggatgcgg tcgaagtacc ggctccggct gcggttctgg gtggcccgga gccgctgatg 1081 caggctaccg catggctgaa cgcatatttt caccagccag aggcgattga agagttccct 1141 gtaccggccc tgcatcaccc ggtatttcag caggaatcct ttacccgtca ggtactgtgg 1201 aaactgctga aagtagtgaa attcggcgag gtgatctcct attcccatct ggcggcgctg 1261 gctggcaacc cagcggcaac tgcggcggtg aaaaccgctc tgtctggtaa tccggtgcca 1321 attctgatcc cgtgccaccg tgtggttcag ggcgacctgg acgtaggtgg ctacgaaggc 1381 ggcctggctg tgaaagaatg gctgctggcg cacgaaggtc accgtctggg taaaccaggc 1441 ctgggcggcc gcatggccca ggtgcagctg gttgagagcg gcggtgctct ggtgcagccg 1501 ggcggtagcc tgcgtctgag ctgcgccgcg agcggcttcc cggtgaaccg ctacagcatg 1561 cgttggtacc gtcaggcgcc gggcaaggag cgtgaatggg tggctggtat gagcagcgcc 1621 ggtgaccgta gcagctacga agatagcgtg aaaggccgct tcaccatcag ccgtgacgat 1681 gcccgcaaca ccgtgtatct gcagatgaac agcctgaagc cggaggacac cgcggtgtac 1741 tattgcaacg tgaacgtggg ttttgaatac tggggccagg gtacccaggt gaccgtgagc 1801 agcaagtccg gactcagatc tcgagctgat ccaaaaaaga agagaaaggt agatccaaaa 1861 aagaagagaa aggtagatcc aaaaaagaag agaaaggtag gatccaccgg atctagatag 1921 taaggatcca cccagctttc ttgtacaaag tggtgatcgc gtaaactacg ggctgcagga 1981 attccgcccc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc 2041 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa 2101 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat 2161 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac 2221 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg 2281 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt 2341 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg 2401 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac 2461 atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac 2521 gtggttttcc tttgaaaaac acgatgataa taccatggtg agcaagggcg aggagctgtt 2581 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 2641 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 2701 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt 2761 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 2821 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 2881 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 2941 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 3001 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 3061 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 3121 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag 3181 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 3241 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgac tagctagtag 3301 ctaactcgag catgcatcta gagggcccta ttctatagtg tcacctaaat gctagagctc 3361 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 3421 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 3481 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 3541 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 3601 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg 3661 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 3721 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 3781 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc 3841 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 3901 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 3961 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga 4021 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct 4081 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta 4141 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 4201 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 4261 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 4321 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 4381 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 4441 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg 4501 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 4561 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 4621 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc 4681 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 4741 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 4801 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 4861 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 4921 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 4981 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg 5041 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 5101 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 5161 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 5221 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 5281 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt 5341 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg 5401 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt 5461 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 5521 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 5581 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 5641 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 5701 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 5761 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 5821 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 5881 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 5941 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 6001 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 6061 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 6121 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 6181 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct 6241 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 6301 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 6361 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 6421 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 6481 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 6541 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 6601 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 6661 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 6721 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 6781 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 6841 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 6901 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 6961 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 7021 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 7081 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 7141 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 7201 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 7261 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 7321 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 7381 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 7441 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 7501 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 7561 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 7621 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 7681 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 7741 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc //