LOCUS       pCDNA3.0_nucLAMA        8272 bp    DNA     circular UNA 08-NOV-2019
DEFINITION  NLS004_SNAP-LAMA-FG97-NLS  IRES EGFP pcDNA3.0.
ACCESSION   urn.local...3y-b7nwlnf
VERSION     urn.local...3y-b7nwlnf
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        236..852
                     /standard_name="CMV_immearly_promoter"
     enhancer        315..602
                     /note="Geneious type: Enhancer"
                     /standard_name="CAG_enhancer"
     promoter        863..881
                     /standard_name="T7 Promotor"
     misc_feature    890..897
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AGCTT"
     misc_feature    898..900
                     /note="Geneious type: Misc_Feat"
                     /standard_name="kozak"
     CDS             901..1446
                     /standard_name="SNAP"
     misc_feature    948
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     CDS             1453..1761
                     /standard_name="VHH_Enhancer\"
     misc_feature    1761^1762
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="TTT"
     gene            1762..2253
                     /note="Geneious type: Gene"
                     /standard_name="cpDHFRL24G5"
     misc_feature    2229
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="C"
     misc_feature    2254..2256
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     CDS             2257..2295
                     /standard_name="VHH_Enhancer"
     misc_feature    2296..2409
                     /note="Geneious type: Misc_Feat\"
                     /standard_name="NLS "
     misc_feature    2299..2409
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CTGAACCCTCCTGATGAGAGTGGCCCCGGCTGCATGAGCTGC
                     AAGTGTGTGCTCTCC"
     misc_feature    complement(2425..2445)
                     /note="Geneious type: attB2"
                     /standard_name="attB2"
     misc_feature    2473..3045
                     /note="Geneious type: Misc_Feat"
                     /standard_name="IRES"
     misc_feature    3046..3793
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="ATGGCTAGCAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCA
                     ATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGG
                     GTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAA
                     ACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCTCTTATGGTGTTCAATGCTTT
                     TCAAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAG
                     GTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGC
                     TGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGAT
                     TTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACA
                     ATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAG
                     ACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCA
                     ATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCC
                     TTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGC
                     TGCTGGGATTACACATGGCATGGATGAACTATACAAA"
     CDS             3047..3792
                     /standard_name="EGFP"
     promoter        complement(3826..3843)
                     /standard_name="SP6 promotor"
     terminator      3865..4092
                     /standard_name="bGH_PA_terminator"
     polyA_site      3870..4059
     rep_origin      4155..4461
     enhancer        complement(4581..4797)
                     /note="Geneious type: Enhancer"
                     /standard_name="SV40_enhancer"
     promoter        4593..4862
                     /standard_name="SV40_promoter"
     rep_origin      complement(4761..4838)
     CDS             4980..5768
                     /standard_name="NeoR/KanR"
     terminator      5948..6067
                     /standard_name="SV40_PA_terminator"
     promoter        complement(6164..6193)
                     /standard_name="lac_promoter"
     rep_origin      complement(6502..6821)
     CDS             complement(7296..8136)
                     /standard_name="Amp"
     promoter        complement(8178..8206)
                     /standard_name="AmpR Promotor"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccag cggccgcacc
      901 atggataaag actgcgaaat gaaacgcact accctggaca gcccgcttgg taaactggaa
      961 ctgagcggtt gcgagcaggg cctgcacgaa atcattttcc tgggcaaagg tacgagcgct
     1021 gcggatgcgg tcgaagtacc ggctccggct gcggttctgg gtggcccgga gccgctgatg
     1081 caggctaccg catggctgaa cgcatatttt caccagccag aggcgattga agagttccct
     1141 gtaccggccc tgcatcaccc ggtatttcag caggaatcct ttacccgtca ggtactgtgg
     1201 aaactgctga aagtagtgaa attcggcgag gtgatctcct attcccatct ggcggcgctg
     1261 gctggcaacc cagcggcaac tgcggcggtg aaaaccgctc tgtctggtaa tccggtgcca
     1321 attctgatcc cgtgccaccg tgtggttcag ggcgacctgg acgtaggtgg ctacgaaggc
     1381 ggcctggctg tgaaagaatg gctgctggcg cacgaaggtc accgtctggg taaaccaggc
     1441 ctgggcggcc gcatggccca ggtgcagctg gttgagagcg gcggtgctct ggtgcagccg
     1501 ggcggtagcc tgcgtctgag ctgcgccgcg agcggcttcc cggtgaaccg ctacagcatg
     1561 cgttggtacc gtcaggcgcc gggcaaggag cgtgaatggg tggctggtat gagcagcgcc
     1621 ggtgaccgta gcagctacga agatagcgtg aaaggccgct tcaccatcag ccgtgacgat
     1681 gcccgcaaca ccgtgtatct gcagatgaac agcctgaagc cggaggacac cgcggtgtac
     1741 tattgcaacg tgaacgtggg tctgcctgcc gatctcgcct ggtttaaacg caacacctta
     1801 aataaacccg tgattatggg ccgccatacc tgggaatcaa tcggtcgtcc gttgccagga
     1861 cgcaaaaata ttatcctcag cagtcaaccg ggtacggacg atcgcgtaac gtgggtgaag
     1921 tcggtggatg aagccatcgc ggcgtgtggt gacgtaccag aaatcatggt gattggcggc
     1981 ggtcgcgttt atgaacagtt cttgccaaaa gcgcaaaaac tgtatctgac gcatatcgac
     2041 gcagaagtgg aaggcgacac ccatttcccg gattacgagc cggatgactg ggaatcggta
     2101 ttcagcgaat tccacgatgc tgatgcgcag aactctcaca gctattgctt tgagattctg
     2161 gagcggcggg gcggcggcgg cggcatgatc agtctgattg cggcgttagc ggtagatcgc
     2221 gttatcggaa tggaaaacgc catgccgtgg aactttgaat actggggcca gggtacccag
     2281 gtgaccgtga gcagcaagtc cggactcaga tctcgagctg atccaaaaaa gaagagaaag
     2341 gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt aggatccacc
     2401 ggatctagat agtaaggatc cacccagctt tcttgtacaa agtggtgatc gcgtaaacta
     2461 cgggctgcag gaattccgcc cccccccccc taacgttact ggccgaagcc gcttggaata
     2521 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     2581 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     2641 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     2701 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     2761 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     2821 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     2881 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     2941 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg
     3001 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aataccatgg tgagcaaggg
     3061 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     3121 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     3181 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     3241 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     3301 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     3361 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     3421 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     3481 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     3541 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     3601 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     3661 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3721 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctcg
     3781 actagctagt agctaactcg agcatgcatc tagagggccc tattctatag tgtcacctaa
     3841 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     3901 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     3961 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4021 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4081 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     4141 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4201 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4261 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg
     4321 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4381 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4441 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4501 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4561 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg
     4621 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4681 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4741 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4801 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4861 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     4921 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     4981 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     5041 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     5101 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     5161 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5221 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     5281 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     5341 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     5401 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     5461 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     5521 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     5581 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5641 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5701 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5761 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     5821 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5881 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     5941 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6001 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6061 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     6121 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6181 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6241 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6301 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6361 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6421 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6481 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6541 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6601 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6661 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     6721 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6781 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6841 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6901 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     6961 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7021 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7081 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7141 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7201 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7261 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7321 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7381 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7441 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7501 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7561 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7621 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7681 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7741 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7801 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7861 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7921 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7981 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8041 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8101 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8161 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8221 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
