LOCUS       pCDNA3.0_mitoSNA        6040 bp    DNA     circular UNA 07-NOV-2019
DEFINITION  Ntom012_NTOM20-SNAP pcDNA3.0.
ACCESSION   urn.local...3z-b7nwlng
VERSION     urn.local...3z-b7nwlng
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        236..852
                     /standard_name="CMV_immearly_promoter"
     enhancer        315..602
                     /note="Geneious type: Enhancer"
                     /standard_name="CAG_enhancer"
     promoter        863..881
                     /standard_name="T7 Promotor"
     misc_feature    890..897
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AGCTT"
     misc_feature    898..900
                     /note="Geneious type: Misc_Feat"
                     /standard_name="kozak"
     misc_feature    901..903
                     /note="Geneious type: Misc_Feat\"
                     /standard_name="Start"
     misc_feature    904..1011
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    904..1005
                     /standard_name="NTOM20"
     CDS             1012..1554
                     /standard_name="SNAP"
     misc_feature    1056
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     misc_feature    1554^1555
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GGCCGCATGGCCCAGGTGCAGCTGGTTGAGAGCGGCGGTGCT
                     CTGGTGCAGCCGGGCGGTAGCCTGCGTCTGAGCTGCGCCGCGAGCGGCTTCCCGGTGA
                     ACCGCTACAGCATGCGTTGGTACCGTCAGGCGCCGGGCAAGGAGCGTGAATGGGTGGC
                     TGGTATGAGCAGCGCCGGTGACCGTAGCAGCTACGAAGATAGCGTGAAAGGCCGCTTC
                     ACCATCAGCCGTGACGATGCCCGCAACACCGTGTATCTGCAGATGAACAGCCTGAAGC
                     CGGAGGACACCGCGGTGTACTATTGCAACGTGAACGTGGGTTTTCTGCCTGCCGATCT
                     CGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGG
                     GAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGG
                     GTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGG
                     TGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCA
                     AAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAAGGCGACACCCATT
                     TCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGA
                     TGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGGGCGGCGGCGGC
                     GGCATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACG
                     CCATGCCGTGGAACGAATACTGGGGCCAGGGTACCCAGGTGACCGTGAGCAGCAAGCT
                     GAACCCTCCTGATGAGAGTGGCCCCGGCTGCATGAGCTGCAAGTGTGTGCTCTCC"
     misc_feature    1561
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="GGATCCACCCAGCTTTCTTGTACAAAGTGGTGATCGCGTAAA
                     CTACGGGCTGCAGGAATTCCGCCCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTG
                     GAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTG
                     GCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCT
                     TTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCT
                     CTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACC
                     CCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGC
                     AAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAA
                     TGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATT
                     GTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTT
                     AAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGAT
                     GATAATACATGGCTAGCAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGT
                     TGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGT
                     GATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTG
                     TTCCATGGCCAACACTTGTCACTACTTTCTCTTATGGTGTTCAATGCTTTTCAAGATA
                     CCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTA
                     CAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCA
                     AGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGA
                     AGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATAC
                     ATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACA
                     TTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGA
                     TGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAA
                     GATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGA
                     TTACACATGGCATGGATGAACTATACAAA"
     promoter        complement(1594..1611)
                     /standard_name="SP6 promotor"
     terminator      1633..1860
                     /standard_name="bGH_PA_terminator"
     polyA_site      1638..1827
     rep_origin      1923..2229
     enhancer        complement(2349..2565)
                     /note="Geneious type: Enhancer"
                     /standard_name="SV40_enhancer"
     promoter        2361..2630
                     /standard_name="SV40_promoter"
     rep_origin      complement(2529..2606)
     CDS             2748..3536
                     /standard_name="NeoR/KanR"
     terminator      3716..3835
                     /standard_name="SV40_PA_terminator"
     promoter        complement(3932..3961)
                     /standard_name="lac_promoter"
     rep_origin      complement(4270..4589)
     CDS             complement(5064..5904)
                     /standard_name="Amp"
     promoter        complement(5946..5974)
                     /standard_name="AmpR Promotor"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccag cggccgcacc
      901 atggtgggcc gcaacagcgc gattgcagct ggagtgtgcg gcgcgctgtt cattggctat
      961 tgcatttact ttgatcgcaa acgccgcagc gatccgaact ttaaatctag agataaagac
     1021 tgcgaaatga aacgcactac cctggacagc ccgcttggta aactggaact gagcggttgc
     1081 gagcagggcc tgcacgaaat cattttcctg ggcaaaggta cgagcgctgc ggatgcggtc
     1141 gaagtaccgg ctccggctgc ggttctgggt ggcccggagc cgctgatgca ggctaccgca
     1201 tggctgaacg catattttca ccagccagag gcgattgaag agttccctgt accggccctg
     1261 catcacccgg tatttcagca ggaatccttt acccgtcagg tactgtggaa actgctgaaa
     1321 gtagtgaaat tcggcgaggt gatctcctat tcccatctgg cggcgctggc tggcaaccca
     1381 gcggcaactg cggcggtgaa aaccgctctg tctggtaatc cggtgccaat tctgatcccg
     1441 tgccaccgtg tggttcaggg cgacctggac gtaggtggct acgaaggcgg cctggctgtg
     1501 aaagaatggc tgctggcgca cgaaggtcac cgtctgggta aaccaggcct gggctagtaa
     1561 ctaactcgag catgcatcta gagggcccta ttctatagtg tcacctaaat gctagagctc
     1621 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     1681 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     1741 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     1801 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     1861 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     1921 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     1981 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2041 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc
     2101 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     2161 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2221 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga
     2281 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     2341 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta
     2401 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     2461 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     2521 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     2581 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     2641 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     2701 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     2761 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     2821 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     2881 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     2941 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     3001 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     3061 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     3121 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     3181 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     3241 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     3301 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     3361 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     3421 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     3481 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     3541 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     3601 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     3661 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     3721 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3781 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     3841 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     3901 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     3961 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     4021 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     4081 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4141 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4201 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4261 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4321 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4381 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     4501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4621 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4681 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     4741 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4801 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4861 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4921 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4981 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5041 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5101 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5161 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5221 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5281 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     5341 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     5401 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     5461 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5521 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     5581 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     5641 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5701 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     5761 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     5821 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     5881 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     5941 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6001 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
