LOCUS       pCDNA3.0_LynSNAP        7369 bp    DNA     circular UNA 08-NOV-2019
DEFINITION  Lyn004_Lyn11-SNAP IRES EGFP pcDNA3.0.
ACCESSION   urn.local...44-b7nwlnp
VERSION     urn.local...44-b7nwlnp
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        236..852
                     /standard_name="CMV_immearly_promoter"
     enhancer        315..602
                     /note="Geneious type: Enhancer"
                     /standard_name="CAG_enhancer"
     promoter        863..881
                     /standard_name="T7 Promotor"
     misc_feature    890..897
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AGCTT"
     misc_feature    898..900
                     /note="Geneious type: Misc_Feat"
                     /standard_name="kozak"
     misc_feature    901..>963
                     /note="Geneious type: ORF"
                     /standard_name="orf"
     misc_feature    901..>903
                     /standard_name="START"
     CDS             901..942
                     /standard_name="Lyn_11"
     misc_feature    904..963
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     CDS             943..963
                     /standard_name="Linker"
     CDS             <964..1506
                     /codon_start=3
                     /standard_name="SNAP-tag"
     misc_feature    1008
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     misc_feature    1506^1507
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GGCCGCATGGCCCAGGTGCAGCTGGTTGAGAGCGGCGGTGCT
                     CTGGTGCAGCCGGGCGGTAGCCTGCGTCTGAGCTGCGCCGCGAGCGGCTTCCCGGTGA
                     ACCGCTACAGCATGCGTTGGTACCGTCAGGCGCCGGGCAAGGAGCGTGAATGGGTGGC
                     TGGTATGAGCAGCGCCGGTGACCGTAGCAGCTACGAAGATAGCGTGAAAGGCCGCTTC
                     ACCATCAGCCGTGACGATGCCCGCAACACCGTGTATCTGCAGATGAACAGCCTGAAGC
                     CGGAGGACACCGCGGTGTACTATTGCAACGTGAACGTGGGTTTTCTGCCTGCCGATCT
                     CGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGG
                     GAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGG
                     GTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGG
                     TGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCA
                     AAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAAGGCGACACCCATT
                     TCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGA
                     TGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGGGCGGCGGCGGC
                     GGCATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACG
                     CCATGCCGTGGAACGAATACTGGGGCCAGGGTACCCAGGTGACCGTGAGCAGCAAGCT
                     GAACCCTCCTGATGAGAGTGGCCCCGGCTGCATGAGCTGCAAGTGTGTGCTCTCC"
     misc_feature    complement(1522..1542)
                     /note="Geneious type: attB2"
                     /standard_name="attB2"
     misc_feature    1570..2142
                     /note="Geneious type: Misc_Feat"
                     /standard_name="IRES"
     misc_feature    2143..2890
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="ATGGCTAGCAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCA
                     ATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGG
                     GTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAA
                     ACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCTCTTATGGTGTTCAATGCTTT
                     TCAAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAG
                     GTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGC
                     TGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGAT
                     TTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACA
                     ATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAG
                     ACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCA
                     ATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCC
                     TTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGC
                     TGCTGGGATTACACATGGCATGGATGAACTATACAAA"
     CDS             2144..2889
                     /standard_name="EGFP"
     promoter        complement(2923..2940)
                     /standard_name="SP6 promotor"
     terminator      2962..3189
                     /standard_name="bGH_PA_terminator"
     polyA_site      2967..3156
     rep_origin      3252..3558
     enhancer        complement(3678..3894)
                     /note="Geneious type: Enhancer"
                     /standard_name="SV40_enhancer"
     promoter        3690..3959
                     /standard_name="SV40_promoter"
     rep_origin      complement(3858..3935)
     CDS             4077..4865
                     /standard_name="NeoR/KanR"
     terminator      5045..5164
                     /standard_name="SV40_PA_terminator"
     promoter        complement(5261..5290)
                     /standard_name="lac_promoter"
     rep_origin      complement(5599..5918)
     CDS             complement(6393..7233)
                     /standard_name="Amp"
     promoter        complement(7275..7303)
                     /standard_name="AmpR Promotor"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccag cggccgcacc
      901 atgggatgta taaaatcaaa agggaaagac agcgcgggag cagatagtgc tggtagtgct
      961 ggtgataaag actgcgaaat gaaacgcact accctggaca gcccgcttgg taaactggaa
     1021 ctgagcggtt gcgagcaggg cctgcacgaa atcattttcc tgggcaaagg tacgagcgct
     1081 gcggatgcgg tcgaagtacc ggctccggct gcggttctgg gtggcccgga gccgctgatg
     1141 caggctaccg catggctgaa cgcatatttt caccagccag aggcgattga agagttccct
     1201 gtaccggccc tgcatcaccc ggtatttcag caggaatcct ttacccgtca ggtactgtgg
     1261 aaactgctga aagtagtgaa attcggcgag gtgatctcct attcccatct ggcggcgctg
     1321 gctggcaacc cagcggcaac tgcggcggtg aaaaccgctc tgtctggtaa tccggtgcca
     1381 attctgatcc cgtgccaccg tgtggttcag ggcgacctgg acgtaggtgg ctacgaaggc
     1441 ggcctggctg tgaaagaatg gctgctggcg cacgaaggtc accgtctggg taaaccaggc
     1501 ctgggctagt aaggatccac ccagctttct tgtacaaagt ggtgatcgcg taaactacgg
     1561 gctgcaggaa ttccgccccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     1621 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     1681 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     1741 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     1801 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     1861 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     1921 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     1981 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     2041 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac
     2101 cacggggacg tggttttcct ttgaaaaaca cgatgataat accatggtga gcaagggcga
     2161 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2221 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2281 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2341 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2401 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2461 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2521 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2581 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2641 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     2701 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     2761 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2821 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca gatctcgact
     2881 agctagtagc taactcgagc atgcatctag agggccctat tctatagtgt cacctaaatg
     2941 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     3001 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     3061 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     3121 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     3181 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     3241 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3301 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3361 ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt
     3421 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     3481 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3541 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3601 ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3661 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag
     3721 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3781 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3841 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3901 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     3961 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     4021 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     4081 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     4141 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     4201 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     4261 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     4321 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     4381 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     4441 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     4501 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     4561 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     4621 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     4681 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     4741 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     4801 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     4861 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     4921 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     4981 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     5041 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5101 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5161 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     5221 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     5281 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     5341 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5401 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5461 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5521 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5581 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5641 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5701 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5761 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
     5821 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5881 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5941 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6001 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     6061 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6121 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6181 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6241 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6301 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6361 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6421 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6481 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6541 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6601 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6661 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6721 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6781 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     6841 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     6901 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     6961 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7021 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7081 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7141 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7201 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7261 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7321 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
