LOCUS       pET51b(+)_EGFP-L        6769 bp    DNA     circular UNA 08-NOV-2019
DEFINITION  Al012_EGFP-LAMA-R59 (for ALFA-tag) in pet51b(+) vector .
ACCESSION   urn:local:.:1513543593640.809
VERSION     urn:local:.:1513543593640.809
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             complement(183..374)
     CDS             complement(946..2028)
     promoter        complement(2029..2106)
     promoter        2419..2437
     protein_bind    2438..2462
     misc_feature    2515..2538
                     /standard_name="StrepTag"
     misc_feature    2545..2559
                     /standard_name="Enterokinase"
     gene            2569..3285
                     /standard_name="EGFP"
     misc_feature    3286..3291
                     /standard_name="Linker"
     CDS             3292..3462
                     /standard_name="NbALFA(superTight)"
     misc_feature    3293..4149
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CCCAGGTTCAGCTTGTGGAGTCTGGTGGAGGCTTGGTACAAG
                     CTGGTGGTAGTCTGCGCTTATCGTGTGCGGCATCAGGCTCGTTCTTTATGAGCAATGT
                     CATGGCGTGGTATCGTCAGGCTCCAGGCAAAGCACGGGAACTCATTGCCGCGATTCGT
                     GGTGGGGATATGAGCACCGTGTATGACGATAGCGTGAAAGGCCGCTTTACGATCACAC
                     GCGATGATGACAAGAACATCCTGTATCTGCAGATGAACGACCTGAAACCGGAAGATAC
                     CGCCATGTACTACTGCAAAGCGAGTGGGTCCTCTTGGGGCCAAGGAACTCAGGTTACC
                     GTCAGCTCC"
     gene            3463..3954
                     /standard_name="cpDHFRL24G5"
     CDS             <3955..4149
                     /standard_name="NbALFA_Supertight"
     misc_feature    4180..4209
                     /standard_name="His-Tag"
     misc_feature    4210..4212
                     /standard_name="Stop"
     terminator      4246..4293
     rep_origin      4330..4785
     CDS             complement(4900..5760)
     promoter        complement(5761..5853)
     rep_origin      5934..6522
     misc_feature    6708..81
ORIGIN      
        1 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
       61 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
      121 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
      181 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
      241 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
      301 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
      361 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
      421 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
      481 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
      541 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag
      601 cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
      661 agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt
      721 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
      781 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gctagtcatg
      841 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat
      901 cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc
      961 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1021 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
     1081 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
     1141 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
     1201 ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat
     1261 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
     1321 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
     1381 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
     1441 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
     1501 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
     1561 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
     1621 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
     1681 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
     1741 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
     1801 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
     1861 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     1921 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
     1981 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     2041 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     2101 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     2161 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     2221 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     2281 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     2341 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg
     2401 atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt
     2461 cccctctaga aataattttg tttaacttta agaaggagat ataccatggc aagctggagc
     2521 cacccgcagt tcgaaaaggg tgcagatgac gacgacaagg taccgcatat ggtgagcaag
     2581 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     2641 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     2701 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     2761 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     2821 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     2881 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     2941 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     3001 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     3061 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     3121 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     3181 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3241 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagagcgg cgaagtgcag
     3301 cttcaggaga gtggtggtgg cttggtgcag ccaggtggct ccttacggct gtcctgtacc
     3361 gcatctggcg ttacgattag cgcgctgaat gcgatggcta tggggtggta tcgccaagca
     3421 ccgggagaac gtcgtgtgat ggtagccgct gtatcggaac gtctgcctgc cgatctcgcc
     3481 tggtttaaac gcaacacctt aaataaaccc gtgattatgg gccgccatac ctgggaatca
     3541 atcggtcgtc cgttgccagg acgcaaaaat attatcctca gcagtcaacc gggtacggac
     3601 gatcgcgtaa cgtgggtgaa gtcggtggat gaagccatcg cggcgtgtgg tgacgtacca
     3661 gaaatcatgg tgattggcgg cggtcgcgtt tatgaacagt tcttgccaaa agcgcaaaaa
     3721 ctgtatctga cgcatatcga cgcagaagtg gaaggcgaca cccatttccc ggattacgag
     3781 ccggatgact gggaatcggt attcagcgaa ttccacgatg ctgatgcgca gaactctcac
     3841 agctattgct ttgagattct ggagcggcgg ggcggcggcg gcggcatgat cagtctgatt
     3901 gcggcgttag cggtagatcg cgttatcggc atggaaaacg ccatgccgtg gaacggcaat
     3961 gccatgtatc gcgaaagcgt tcaaggccgc tttaccgtta cccgcgattt cacgaacaaa
     4021 atggtcagtc tgcagatgga caacctgaaa ccggaggata cagcggtgta ctactgccat
     4081 gtcctcgaag atcgcgttga tagctttcac gactattggg gtcaagggac ccaggtcact
     4141 gtgtcgtcag gagctccggg cttctcctca atttccgctc atcaccacca tcatcaccat
     4201 caccaccact aattaaccta ggctgctgcc accgctgagc aataactagc ataacccctt
     4261 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg
     4321 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4381 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4441 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4501 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4561 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4621 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4681 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4741 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttctggc ggcacgatgg
     4801 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4861 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4921 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4981 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5041 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5101 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5161 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5221 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5281 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5341 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5401 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5461 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5521 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5581 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5641 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5701 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5761 actcttcctt tttcaatcat gattgaagca tttatcaggg ttattgtctc atgagcggat
     5821 acatatttga atgtatttag aaaaataaac aaataggtca tgaccaaaat cccttaacgt
     5881 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5941 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6001 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6061 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     6121 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6181 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6241 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6301 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6361 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6421 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6481 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6541 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6601 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6661 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     6721 tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcac
//
