LOCUS       pET51b(+)_ALFA-t        5785 bp    DNA     circular UNA 08-NOV-2019
DEFINITION  AlT002_ALFAPep-SNAP in pET51b(+) vector.
ACCESSION   urn:local:.:1513579385120.839
VERSION     urn:local:.:1513579385120.839
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             complement(183..374)
                     /standard_name="Rop PRotein"
     CDS             complement(946..2028)
                     /standard_name="Lac repressor"
     promoter        complement(2029..2106)
                     /standard_name="Lac1"
     promoter        2419..2437
                     /standard_name="t7 promotor"
     protein_bind    2438..2462
                     /standard_name="LacOperon"
     misc_feature    2515..2538
                     /standard_name="StrepTag"
     misc_feature    2545..2559
                     /standard_name="Enterokinase"
     misc_feature    2569..2619
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    2569..2613
                     /note="Geneious type: Misc_Feat"
                     /standard_name="ALFA-tag Peptide"
     misc_feature    2614..2619
                     /standard_name="Linker"
     gene            2620..3165
                     /standard_name="SNAP"
     misc_feature    3165^3166
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="AGCGGCCCTATAGTGCAGAACCTCCAGGGGCAAATGGTACAT
                     CAGGCCATATCACCTAGAACTTTAAATGCATGGGTAAAAGTAGTAGAAGAGAAGGCTT
                     TCAGCCCAGAAGTAATACCCATGTTTTCAGCATTATCAGAAGGAGCCACCCCACAAGA
                     TTTAAATACCATGCTAAACACAGTGGGGGGACATCAAGCAGCCATGCAAATGTTAAAA
                     GAGACCATCAATGAGGAAGCTGCAGAATGGGATAGATTGCATCCAGTGCATGCAGGGC
                     CTATTGCACCAGGCCAGATGAGAGAACCAAGGGGAAGTGACATAGCAGGAACTACTAG
                     TACCCTTCAGGAACAAATAGGATGGATGACACATAATCCACCTATCCCAGTAGGAGAA
                     ATCTATAAAAGATGGATAATCCTGGGATTAAATAAAATAGTAAGAATGTATAGC"
     misc_feature    3196..3225
                     /standard_name="His-Tag"
     misc_feature    3226..3228
                     /standard_name="Stop"
     terminator      3262..3309
     rep_origin      3346..3801
     CDS             complement(3916..4776)
                     /standard_name="Beta lacamase"
     promoter        complement(4777..4869)
     rep_origin      4950..5538
     misc_feature    5724..81
                     /standard_name="boom"
ORIGIN      
        1 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
       61 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
      121 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
      181 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
      241 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
      301 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
      361 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
      421 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
      481 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
      541 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag
      601 cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
      661 agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt
      721 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
      781 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gctagtcatg
      841 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat
      901 cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc
      961 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1021 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
     1081 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
     1141 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
     1201 ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat
     1261 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
     1321 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
     1381 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
     1441 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
     1501 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
     1561 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
     1621 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
     1681 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
     1741 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
     1801 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
     1861 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     1921 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
     1981 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     2041 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     2101 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     2161 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     2221 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     2281 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     2341 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg
     2401 atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt
     2461 cccctctaga aataattttg tttaacttta agaaggagat ataccatggc aagctggagc
     2521 cacccgcagt tcgaaaaggg tgcagatgac gacgacaagg taccgcatcc gagccgcctg
     2581 gaagaagaac tgcgccgccg cctgaccgaa ccgagcggca tggataaaga ctgcgaaatg
     2641 aaacgcacta ccctggacag cccgctgggt aaactggaac tgagcggttg cgagcagggc
     2701 ctgcacgaaa tcattttcct gggcaaaggt acgagcgctg cggatgcggt cgaagtaccg
     2761 gctccggctg cggttctggg tggcccggag ccgctgatgc aggctaccgc atggctgaac
     2821 gcatattttc accagccaga ggcgattgaa gagttccctg taccggccct gcatcacccg
     2881 gtatttcagc aggaatcctt tacccgtcag gtactgtgga aactgctgaa agtagtgaaa
     2941 ttcggcgagg tgatctccta ttcccatctg gcggcgctgg ctggcaaccc agcggcaact
     3001 gcggcggtga aaaccgctct gtctggtaat ccggtgccaa ttctgatccc gtgccaccgt
     3061 gtggttcagg gcgacctgga cgtaggtggc tacgaaggcg gcctggctgt gaaagaatgg
     3121 ctgctggcgc acgaaggtca ccgtctgggt aaaccaggcc tgggcggagc tccgggcttc
     3181 tcctcaattt ccgctcatca ccaccatcat caccatcacc accactaatt aacctaggct
     3241 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     3301 ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg
     3361 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3421 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3481 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3541 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3601 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3661 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     3721 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3781 aaatattaac gtttacaatt tctggcggca cgatggcatg agattatcaa aaaggatctt
     3841 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3901 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3961 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4021 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4081 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4141 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4201 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4261 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4321 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4381 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4441 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4501 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4561 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4621 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4681 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4741 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatcatgatt
     4801 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4861 ataaacaaat aggtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4921 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4981 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5041 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5101 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5161 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5221 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5281 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5341 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5401 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5461 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5521 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5581 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5641 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5701 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     5761 atttcacacc gcatatatgg tgcac
//
