LOCUS       Exported                9081 bp ds-DNA     circular SYN 16-JAN-2015
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 20, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9081
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..180
                     /label=5' LTR
                     /note="color: #ff80c0"
     misc_feature    241..353
                     /label=Psi packaging signal
                     /note="color: #00ffff"
     misc_feature    786..1140
                     /label=Rev Response Element (RRE)
                     /note="color: #00ffff"
     misc_feature    1995..2405
                     /label=blasticidin resistence
                     /note="color: #ffff80"
     misc_feature    2442..2474
                     /label=central pulypurine tract (cPPT)
                     /note="color: #00ffff"
     misc_feature    2626..3187
                     /label=CMV
                     /note="color: #cf9fff"
     misc_feature    3214..3238
                     /label=attB1
                     /note="color: #8080ff"
     misc_feature    3276..4592
                     /label=Kir2.1
                     /note="color: #ff8080"
     misc_feature    4599..4622
                     /label=attB2
                     /note="color: #8080ff"
     misc_feature    4707..5294
                     /label=WPRE
                     /note="color: #00ffff"
     misc_feature    5339..5370
                     /label=popypurine tract (PPT)
                     /note="color: #00ffff"
     misc_feature    5420..5599
                     /label=3' LTR
                     /note="color: #ff80c0"
     misc_feature    5681..5800
                     /label=SV40 polyA terminator
                     /note="color: #00ffff"
     misc_feature    6767..7627
                     /label=AmpR
                     /note="color: #ffff80"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc
     1501 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     1561 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     1621 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     1681 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     1741 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     1801 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     1861 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     1921 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     1981 cagggggtac caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     2041 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     2101 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     2161 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     2221 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     2281 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     2341 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ctgcagctgc
     2401 agtagtaaga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     2461 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2521 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     2581 gggacagcag agatccactt tggcgccggc tcgagggggc ccggggttat taatagtaat
     2641 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     2701 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     2761 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     2821 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     2881 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     2941 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3001 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3061 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3121 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     3181 taagcagagc tctctggcta actgtcggga tcaacaagtt tgtacaaaaa agcaggctgg
     3241 cgccggaacc aattcagtcg actggatccg ccaccatgcc aactttgtat acaaaagttg
     3301 ccgccatggg cagtgtgaga accaaccgct acagcatcgt ctcttcggag gaagatggca
     3361 tgaagctggc cactatggca gttgccaatg gctttgggaa tggcaagagt aaagtccata
     3421 cccgacaaca gtgcaggagc cgctttgtga agaaagatgg tcattgcaat gttcagttta
     3481 tcaacgtggg tgagaaggga cagaggtacc tggcagacat ctttactacc tgtgtcgaca
     3541 tccgctggag gtggatgctg gttatcttct gtcttgcctt cgtgctctcc tggctgttct
     3601 ttggctgtgt gttttggttg atagccctgc tccatgggga tctagatact tctaaagtga
     3661 gcaaagcatg cgtgtcagag gtcaacagct tcacggctgc cttcctcttc tccatcgaga
     3721 cccagacaac cattggctat ggtttcaggt gtgtgacaga cgagtgccca attgctgtct
     3781 tcatggtggt attccagtca atcgtaggct gcatcattga cgccttcatc attggtgcag
     3841 tcatggcgaa gatggcaaag ccaaagaaga gaaatgagac tcttgtcttc agtcacaatg
     3901 ctgtgattgc catgagggat ggcaaactct gcttgatgtg gagagtgggt aaccttcgaa
     3961 agagccacct tgtggaagct catgtccggg cacagcttct caaatctagg atcacttcag
     4021 aaggggagta tatccctttg gaccagatag acatcaatgt tggttttgat agtggaattg
     4081 accgcatatt tctagtgtcc cccatcacta tcgttcacga aatagatgaa gacagccctt
     4141 tatatgactt gagtaagcag gacattgaca atgcagactt tgaaattgtt gtcatactgg
     4201 aaggcatggt ggaggcgact gccatgacaa ctcaatgccg gagttcgtat ctggccaatg
     4261 aaattctctg gggtcaccgc tatgagccag tgctctttga agagaaacac tactataaag
     4321 tagactattc aagattccat aagacttatg aagtacctaa cacccccctt tgtagtgcca
     4381 gagacttagc agagaagaaa tacatccttt caaatgcaaa ttcattttgc tatgaaaatg
     4441 aagttgccct aacaagcaaa gaggaagagg aggatagtga gaacggagtc ccagagagca
     4501 caagcacaga ctcacctcct ggcatagatc tccacaacca ggcaagcgta cctctagagc
     4561 ccaggccctt aaggcgagaa tcggagatat gatctagacc cagctttctt gtacaaagtg
     4621 gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc
     4681 gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     4741 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4801 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4861 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4921 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4981 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5041 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5101 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5161 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5221 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5281 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
     5341 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     5401 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5461 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5521 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5581 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
     5641 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     5701 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5761 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     5821 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5881 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5941 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     6001 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6061 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6121 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6601 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6661 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6721 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6781 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6841 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6901 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6961 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7021 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7081 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7141 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7201 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7261 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7321 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7381 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7441 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7501 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7561 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7621 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7681 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7741 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7801 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7861 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7921 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7981 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8041 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8101 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8161 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8221 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8281 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8341 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8401 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8461 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8521 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8581 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8641 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8701 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8761 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     8821 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     8881 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     8941 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     9001 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     9061 tgcctagctc gatacataaa c
//
