LOCUS       U6_sgRNA_CAG_hSth1Cas9_CNRZ    8733 bp      DNA     circular   2019-11-20
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : MSP1594_2x_NLS.xdna [8278  nt] : (SpeI[18] / S
            peI[18]) to Sth1Cas9_sgRNA_pUC19.xdna [429  nt] : (NheI[7] /
             SpeI[440])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     promoter        451..2151
                     /label=CAG promoter
                     /SerialCloner_Color=&hFC8008
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - label="CAG promoter" - 18..1718 \ 
     promoter        451..814
                     /label=CMV Enhancer
                     /SerialCloner_Color=&hFC8008
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=SerialCloner_Color="&h03C000" - SerialCloner_Show="True" - SerialCloner_Protect="True" - SerialCloner_Arrow="True" - Color="#ff5555" - modified_by="User" - X-Transferred_From="MSP469; MSP469 updated; MSP680; - MSP977; pCMV-T7-Cas9 (MLM3613); pCMV-T7-Cas9  \ 
     misc_feature    2196..2205
                     /label=Kozak
                     /SerialCloner_Color=&hFDCC65
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - modified_by="User" - X-Transferred_From="BPK2139; MSP680; MSP977" - X-Transferred_Similarity="100.00%" - Transferred_From="BPK2139, MSP1101, MSP680, MSP977" - Transferred_Similarity="100.00%" - label="Kozak" - 1763..1772 \ 
     misc_feature    5631..5651
                     /label=SV40 NLS
                     /SerialCloner_Color=&h00FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - label="SV40 NLS" - 5147..5167 \ 
     terminator      5687..6190
                     /label=RBGpA
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT - LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK - DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN - GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH - MVLLEFVTAAGI \ 
     promoter        complement(6285..6314)
                     /label=lac promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT - LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK - DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN - GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH - MVLLEFVTAAGI \ 
     unsure          6420..6599
                     /label=SV40 promoter
                     /SerialCloner_Color=&hFFD700
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - label="SV40 promoter" - 5921..6100 \ 
     unsure          6455..6532
                     /label=SV40 origin
                     /SerialCloner_Color=&hFFD700
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - Color="#aa00aa" - modified_by="User" - label="SV40 origin" - 5956..6033 \ 
     terminator      6613..6744
                     /label=SV40 PA terminator
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT - LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK - DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN - GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH - MVLLEFVTAAGI \ 
     rep_origin      complement(6968..7587)
                     /label=pBR322 origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT - LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK - DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN - GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH - MVLLEFVTAAGI \ 
     cds             complement(7742..8602)
                     /label=ampR
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Imported_from="<a - /wishart.biology.ualberta.ca/PlasMapper"">Pla - sMapper" - modified_by="User" - created_by="User" - X-Transferred_From="BPK1520; BPK2301; MSP469; MSP680; - MSP977; VVT1; pCAG-hCas9-nls-3XFLAG (SQT817); - pCAG-hCas9-nls-3XFLAG delta Nco \ 
     promoter        complement(8644..8672)
                     /label=amp promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY - IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE - YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL - DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL - LRSALPAGWFIA \ 
     misc_feature    2256..5618
                     /label=St1Cas9 LMD-9:CNRZ_1066 Chimera
                     /SerialCloner_Color=&h0F7FFE
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="SDLVLGLDIGIGSVGVGILNKVTGEIIHKNSRIFPAAQAENNLVRRTNRQGRRLARRKKHRRVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDELSNEELFIALKNMVKHRGISYLDDASDDGNSSVGDYAQIVKENSKQLETKTPGQIQLERYQTYGQLRGDFTVEKDGKKHRLINVFPTSAYRSEALRILQTQQEFNPQITDE \ 
     CDS             complement(7745..8602)
                     /label= bla gene- Ap(r) determinant _AP(R)
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="4" - label=AP(R) - note=" bla gene- Ap(r) determinant " - complement(1629..2486) \ 
     rep_origin      6983..6983
                     /label= RNaseH cleavage point _ORI
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="33" - label=ORI - note=" RNaseH cleavage point " - 867..867 \ 
     CDS             complement(7745..8602)
                     /label=AmpR
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="4" - label=AmpR - complement(1629..2486) \ 
     misc_feature    8603..8667
                     /label=bla\promoter
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="21" - label=blapromoter - 2487..2551 \ 
     promoter        79..341
                     /label=U6 promoter
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    6249..6270
                     /label=M13R-20
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6800..6819
                     /label=pUC19 R
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    363..446
                     /label=crRNA:tracrRNA chimera
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    365..365
                     /label=Wobble correction + U stretch interruption
                     /SerialCloner_Color=&h666666
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2202..2204
                     /label=ATG
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2211..2231
                     /label=SV40 NLS
                     /SerialCloner_Color=&h21FE06
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5658..5663
                     /label=STOPs
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4770..4772
                     /label=A840K
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="K" \ 
     misc_feature    4551..4553
                     /label=D767E
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="E" \ 
     misc_feature    4944..4946
                     /label=I888M
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="M" \ 
     misc_feature    5079..5081
                     /label=L insertion after L941
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="L" \ 
     misc_feature    5088..5090
                     /label=H945P
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="P" \ 
     misc_feature    5115..5117
                     /label=N954K
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="K" \ 
     misc_feature    5154..5156
                     /label=A967T
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="T" \ 
     misc_feature    5139..5141
                     /label=V962L
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="L" \ 
     misc_feature    5142..5144
                     /label=S963K
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="K" \ 
     misc_feature    5175..5177
                     /label=T974A
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="A" \ 
     misc_feature    5145..5147
                     /label=PI Domain start
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="P" \ 
     misc_feature    4692..4694
                     /label=CTD Domain start
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="VDS" \ 
     misc_feature    5400..5402
                     /label=M1049L
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="L" \ 
     misc_feature    5499..5501
                     /label=S1082G
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="G" \ 
     misc_feature    5511..5513
                     /label=K1086I
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="I" \ 
     misc_feature    5523..5525
                     /label=G1090A
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="A" \ 
     misc_feature    5109..5111
                     /label=D952N
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="N" \ 
     misc_feature    5106..5108
                     /label=K951E
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="E" \ 
     misc_feature    343..348
                     /label=BsmBI
                     /SerialCloner_Color=&hFB02FE
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    356..361
                     /label=BsmBI
                     /SerialCloner_Color=&hFB02FE
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4343..4350
                     /label=Sbf1
                     /SerialCloner_Color=&hFC8008
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 gtcgacattg attattgact agctgtacaa aaaagcaggc tttaaaggaa ccaattcagt 
       61 cgactggatc cggtaccaAG GTCGGGCAGG AAGAGGGCCT ATTTCCCATG ATTCCTTCAT 
      121 ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA 
      181 CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG 
      241 TTTTAAAATT ATGTTTTAAA ATGGACTATC Atatgcttac cgtaacttga aagtatttcg 
      301 atttcttggc tttatatatc ttgtggaaag gacgaaacac ccgagacgat taatgcgtct 
      361 cgGTcTTTGT ACTCTGGTAC CAGAAGCTAC AAAGATAAGG CTTCATGCCG AAATCAACAC 
      421 CCTGTCATTT TATGGCAGGG TGTTTTTTTT Actagttatt aatagtaatc aattacgggg 
      481 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 
      541 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 
      601 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 
      661 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 
      721 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 
      781 cagtacatct acgtattagt catcgctatt accatgggtc gaggtgagcc ccacgttctg 
      841 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta 
      901 attattttgt gcagcgatgg gggcgggggg ggggggggcg cgcgccaggc ggggcggggc 
      961 ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg 
     1021 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa 
     1081 gcgcgcggcg ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct 
     1141 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg 
     1201 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt 
     1261 ggctgcgtga aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg 
     1321 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg 
     1381 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag 
     1441 cgcggccggg ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg 
     1501 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc 
     1561 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg 
     1621 tgcggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc 
     1681 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg 
     1741 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc 
     1801 gagagggcgc agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc 
     1861 gccgcacccc ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg 
     1921 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg 
     1981 ctgccgcagg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg 
     2041 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta 
     2101 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattctgc 
     2161 agtcgacggt accgcgggcc cgggatccac cggtcgccac cATGGGCGCC CCAAAGAAGA 
     2221 AGCGGAAGGT CGGTATCCAC GGAGTCCCAG CAGCCAGCGA CCTGGTGCTG GGCCTGGACA 
     2281 TCGGCATCGG CAGCGTGGGC GTGGGCATCC TGAACAAGGT GACCGGCGAG ATCATCCACA 
     2341 AGAACAGTCG CATCTTCCCT GCTGCTCAGG CTGAGAACAA CCTGGTGCGC CGCACCAACC 
     2401 GCCAGGGTCG CCGGCTTGCT CGCCGCAAGA AGCACCGGCG CGTGCGCCTG AACCGCCTGT 
     2461 TCGAGGAGAG CGGCCTGATC ACCGACTTCA CCAAGATCAG CATCAACCTG AACCCCTACC 
     2521 AGCTGCGCGT GAAGGGCCTG ACCGACGAGC TGAGCAACGA GGAGCTGTTC ATCGCCCTGA 
     2581 AGAACATGGT GAAGCACCGC GGCATCAGCT ACCTGGACGA CGCCAGCGAC GACGGCAACA 
     2641 GCAGCGTGGG CGACTACGCC CAGATCGTGA AGGAGAACAG CAAGCAGCTG GAGACCAAGA 
     2701 CCCCCGGCCA GATCCAGCTG GAGCGCTACC AGACCTACGG CCAGCTGCGC GGCGACTTCA 
     2761 CCGTGGAGAA GGACGGCAAG AAGCACCGCC TGATCAACGT GTTCCCCACC AGCGCCTACC 
     2821 GCAGCGAGGC CCTGCGCATC CTGCAGACCC AGCAGGAGTT CAACCCCCAG ATCACCGACG 
     2881 AGTTCATCAA CCGCTACCTG GAGATCCTGA CCGGCAAGCG CAAGTACTAC CACGGCCCCG 
     2941 GCAACGAGAA GAGCCGCACC GACTACGGCC GCTACCGCAC CAGCGGCGAG ACCCTGGACA 
     3001 ACATCTTCGG CATCCTGATC GGCAAGTGCA CCTTCTACCC CGACGAGTTC CGCGCCGCCA 
     3061 AGGCCAGCTA CACCGCCCAG GAGTTCAATC TGCTGAACGA CCTGAACAAC CTGACCGTGC 
     3121 CCACCGAGAC CAAGAAGCTG AGCAAGGAGC AGAAGAACCA GATCATCAAC TACGTGAAGA 
     3181 ACGAGAAGGC TATGGGCCCC GCCAAGCTGT TCAAGTACAT CGCCAAGCTG CTGAGCTGCG 
     3241 ACGTGGCCGA CATCAAGGGC TACCGCATCG ACAAGAGCGG CAAGGCCGAG ATCCACACCT 
     3301 TCGAGGCCTA CCGCAAGATG AAGACCCTGG AGACCCTGGA CATCGAGCAG ATGGACCGAG 
     3361 AGACCCTGGA CAAGCTGGCC TACGTGCTGA CCCTGAACAC CGAGCGCGAG GGCATCCAGG 
     3421 AGGCCCTGGA GCACGAGTTC GCCGACGGCA GCTTCAGCCA GAAACAGGTG GACGAGCTGG 
     3481 TGCAGTTCCG CAAGGCCAAC AGCAGCATCT TCGGCAAGGG CTGGCACAAC TTCAGCGTGA 
     3541 AGCTGATGAT GGAGCTGATC CCCGAGCTGT ACGAGACCAG CGAGGAGCAG ATGACCATCC 
     3601 TGACCCGCCT GGGCAAGCAG AAGACCACCA GCAGCAGCAA CAAGACCAAG TACATCGACG 
     3661 AGAAGCTGCT GACCGAGGAG ATCTACAACC CCGTGGTGGC CAAGAGCGTG CGCCAGGCCA 
     3721 TCAAGATCGT GAACGCCGCC ATCAAGGAGT ACGGCGACTT CGACAACATC GTGATCGAGA 
     3781 TGGCCCGCGA GACCAACGAG GACGACGAGA AGAAGGCCAT CCAGAAGATC CAGAAGGCCA 
     3841 ACAAGGACGA GAAGGACGCC GCCATGCTGA AGGCCGCCAA CCAGTACAAC GGCAAGGCCG 
     3901 AGCTGCCCCA CAGCGTGTTC CACGGCCACA AGCAGCTGGC CACCAAGATC CGCCTGTGGC 
     3961 ACCAGCAGGG CGAGCGCTGC CTGTACACCG GCAAGACCAT CAGCATCCAC GACCTGATCA 
     4021 ACAACAGCAA CCAGTTCGAG GTGGACCACA TCCTGCCCCT GAGCATCACC TTCGACGACA 
     4081 GCCTGGCCAA CAAGGTGCTG GTGTACGCCA CCGCCAACCA GGAGAAGGGC CAGCGCACCC 
     4141 CCTACCAGGC CCTGGACAGC ATGGACGACG CCTGGAGCTT CCGCGAGCTG AAGGCCTTCG 
     4201 TGCGCGAGAG CAAGACCCTG AGCAACAAGA AGAAGGAGTA TCTGCTGACC GAGGAGGACA 
     4261 TCAGCAAGTT CGACGTGCGC AAGAAGTTCA TCGAGCGCAA CCTGGTGGAC ACCCGCTACG 
     4321 CCAGCCGCGT GGTGCTGAAC GCCCTGCAGG AGCACTTCCG CGCCCACAAG ATCGACACCA 
     4381 AGGTGAGCGT GGTGCGCGGC CAGTTCACCA GCCAGCTGCG CCGCCACTGG GGCATCGAGA 
     4441 AGACCCGCGA CACCTACCAC CACCACGCCG TGGACGCCCT GATCATTGCG GCTTCTAGCC 
     4501 AGCTGAACCT GTGGAAGAAG CAGAAGAACA CCCTGGTGAG CTACAGCGAG GAgCAGCTGC 
     4561 TGGACATCGA GACCGGCGAG CTGATCAGCG ACGACGAGTA CAAGGAGAGC GTGTTCAAGG 
     4621 CCCCCTACCA GCACTTCGTG GACACCCTGA AGAGCAAGGA GTTCGAGGAC AGCATCCTGT 
     4681 TCAGCTACCA GGTGGACAGC AAGTTCAACC GCAAGATCAG CGACGCCACC ATCTACGCCA 
     4741 CCCGCCAGGC CAAGGTGGGC AAGGACAAGa agGACGAGAC CTACGTGCTG GGCAAGATCA 
     4801 AGGACATCTA CACCCAGGAC GGCTACGACG CCTTCATGAA GATCTACAAG AAGGACAAGA 
     4861 GCAAGTTCCT GATGTACCGC CACGACCCCC AGACCTTCGA GAAGGTGATC GAGCCCATCC 
     4921 TGGAGAACTA CCCCAACAAG CAGATgAACG AGAAAGGCAA GGAGGTGCCC TGCAACCCCT 
     4981 TCCTGAAGTA CAAGGAGGAG CACGGCTACA TCCGCAAGTA CAGCAAGAAG GGCAACGGCC 
     5041 CCGAGATCAA GAGCCTGAAG TACTACGACA GCAAGCTGct gGGCAACCcC ATCGACATCA 
     5101 CCCCCgAaaA CAGCAAgAAC AAGGTGGTGC TGCAGAGCcT tAagCCCTGG CGCaCCGACG 
     5161 TGTACTTCAA CAAGgCCACC GGCAAGTACG AGATCCTGGG GCTGAAGTAC GCCGATCTGC 
     5221 AGTTTGAGAA AGGCACAGGC ACCTACAAGA TCAGCCAGGA GAAGTACAAC GACATCAAGA 
     5281 AGAAGGAGGG CGTGGACAGC GACAGCGAGT TCAAGTTCAC CCTGTACAAG AACGACCTTC 
     5341 TGCTGGTGAA GGACACCGAG ACCAAGGAGC AACAGCTGTT CCGCTTCCTG AGCCGCACCc 
     5401 TGCCCAAGCA GAAGCACTAC GTGGAGCTGA AGCCCTACGA CAAGCAGAAG TTCGAGGGCG 
     5461 GCGAGGCCCT GATCAAGGTG CTGGGCAACG TGGCCAACgG CGGCCAGTGC AtcAAGGGCC 
     5521 TGGcCAAGAG CAACATCAGC ATCTACAAGG TGCGCACCGA CGTGCTGGGC AACCAGCACA 
     5581 TCATCAAGAA CGAGGGCGAC AAGCCCAAGT TGGACTTCAG CAGGGCTGAC CCCAAGAAGA 
     5641 AGAGGAAGGT GGGATCCTGA TAAgaattcg cggccgcact cctcaggtgc aggctgccta 
     5701 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt 
     5761 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta 
     5821 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg 
     5881 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt 
     5941 ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca 
     6001 gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg 
     6061 ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc 
     6121 cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt 
     6181 ccctcttctc ttatgaagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat 
     6241 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 
     6301 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 
     6361 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta 
     6421 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 
     6481 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 
     6541 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 
     6601 caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     6661 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     6721 aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag 
     6781 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 
     6841 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 
     6901 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 
     6961 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
     7021 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
     7081 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
     7141 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca 
     7201 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
     7261 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
     7321 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
     7381 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 
     7441 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
     7501 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     7561 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     7621 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
     7681 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 
     7741 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 
     7801 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 
     7861 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 
     7921 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 
     7981 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 
     8041 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 
     8101 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 
     8161 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 
     8221 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 
     8281 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 
     8341 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 
     8401 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 
     8461 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 
     8521 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 
     8581 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 
     8641 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 
     8701 ttccgcgcac atttccccga aaagtgccac ctg
//