LOCUS       U6_sgRNA_CAG_hSth1Cas9_MTH17CL396_V2.xdna    8727 bp      DNA     circular   2019-11-20
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : U6_sgRNA_CAG_hSth1Cas9_CNRZ-TH1477.xdna [7931 
             nt] : (AflII[5139] / SbfI[4343]) to PCR(U6_sgRNA_CAG_hSth1C
            as9_LMD9 (1).xdna) [788  nt] : (SbfI[7] / AflII[800])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     promoter        451..2151
                     /label=CAG promoter
                     /SerialCloner_Color=&hFC8008
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - label="CAG promoter" - 18..1718 \ 
     promoter        451..814
                     /label=CMV Enhancer
                     /SerialCloner_Color=&hFC8008
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=SerialCloner_Color="&h03C000" - SerialCloner_Show="True" - SerialCloner_Protect="True" - SerialCloner_Arrow="True" - Color="#ff5555" - modified_by="User" - X-Transferred_From="MSP469; MSP469 updated; MSP680; - MSP977; pCMV-T7-Cas9 (MLM3613); pCMV-T7-Cas9  \ 
     misc_feature    2196..2205
                     /label=Kozak
                     /SerialCloner_Color=&hFDCC65
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - modified_by="User" - X-Transferred_From="BPK2139; MSP680; MSP977" - X-Transferred_Similarity="100.00%" - Transferred_From="BPK2139, MSP1101, MSP680, MSP977" - Transferred_Similarity="100.00%" - label="Kozak" - 1763..1772 \ 
     terminator      5681..6184
                     /label=RBGpA
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT - LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK - DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN - GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH - MVLLEFVTAAGI \ 
     promoter        complement(6279..6308)
                     /label=lac promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT - LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK - DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN - GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH - MVLLEFVTAAGI \ 
     unsure          6414..6593
                     /label=SV40 promoter
                     /SerialCloner_Color=&hFFD700
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - label="SV40 promoter" - 5921..6100 \ 
     unsure          6449..6526
                     /label=SV40 origin
                     /SerialCloner_Color=&hFFD700
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - Color="#aa00aa" - modified_by="User" - label="SV40 origin" - 5956..6033 \ 
     terminator      6607..6738
                     /label=SV40 PA terminator
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT - LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK - DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN - GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH - MVLLEFVTAAGI \ 
     rep_origin      complement(6962..7581)
                     /label=pBR322 origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT - LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK - DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN - GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH - MVLLEFVTAAGI \ 
     cds             complement(7736..8596)
                     /label=ampR
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Imported_from="<a - /wishart.biology.ualberta.ca/PlasMapper"">Pla - sMapper" - modified_by="User" - created_by="User" - X-Transferred_From="BPK1520; BPK2301; MSP469; MSP680; - MSP977; VVT1; pCAG-hCas9-nls-3XFLAG (SQT817); - pCAG-hCas9-nls-3XFLAG delta Nco \ 
     promoter        complement(8638..8666)
                     /label=amp promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY - IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE - YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL - DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL - LRSALPAGWFIA \ 
     CDS             complement(7739..8596)
                     /label= bla gene- Ap(r) determinant _AP(R)
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="4" - label=AP(R) - note=" bla gene- Ap(r) determinant " - complement(1629..2486) \ 
     rep_origin      6977..6977
                     /label= RNaseH cleavage point _ORI
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="33" - label=ORI - note=" RNaseH cleavage point " - 867..867 \ 
     CDS             complement(7739..8596)
                     /label=AmpR
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="4" - label=AmpR - complement(1629..2486) \ 
     misc_feature    8597..8661
                     /label=bla\promoter
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="21" - label=blapromoter - 2487..2551 \ 
     promoter        79..341
                     /label=U6 promoter
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    6243..6264
                     /label=M13R-20
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6794..6813
                     /label=pUC19 R
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    363..446
                     /label=crRNA:tracrRNA chimera
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    365..365
                     /label=Wobble correction + U stretch interruption
                     /SerialCloner_Color=&h666666
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2202..2204
                     /label=ATG
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2211..2231
                     /label=SV40 NLS
                     /SerialCloner_Color=&h21FE06
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5652..5657
                     /label=STOPs
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5142..5144
                     /label=PI Domain start
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="P" \ 
     misc_feature    343..348
                     /label=BsmBI
                     /SerialCloner_Color=&hFB02FE
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    356..361
                     /label=BsmBI
                     /SerialCloner_Color=&hFB02FE
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     5141..5487
                     /label=insert from PCR(U6_sgRNA_CAG_hSth1Cas9_TH1477_AscI_finale.xdna) [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from PCR(U6_sgRNA_CAG_hSth1Cas9_TH1477_AscI_finale.xdna) [525  nt] : (AflII[7] / NotI[535]) \ 
                     5488..5488
                     /label=insert from PCR(U6_sgRNA_CAG_hSth1Cas9_TH1477_AscI_finale.xdna) [*] [5']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from PCR(U6_sgRNA_CAG_hSth1Cas9_TH1477_AscI_finale.xdna) [525  nt] : (AflII[7] / NotI[535]) \ 
                     5491..5498
                     /label=insert from PCR(U6_sgRNA_CAG_hSth1Cas9_TH1477_AscI_finale.xdna) [*] [3'] [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from PCR(U6_sgRNA_CAG_hSth1Cas9_TH1477_AscI_finale.xdna) [525  nt] : (AflII[7] / NotI[535]) \ 
                     5499..5499
                     /label=insert from PCR(U6_sgRNA_CAG_hSth1Cas9_TH1477_AscI_finale.xdna) [*] [3'] [*] [5']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from PCR(U6_sgRNA_CAG_hSth1Cas9_TH1477_AscI_finale.xdna) [525  nt] : (AflII[7] / NotI[535]) \ 
                     5503..5665
                     /label=insert from PCR(U6_sgRNA_CAG_hSth1Cas9_TH1477_AscI_finale.xdna) [*] [3'] [*] [3']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from PCR(U6_sgRNA_CAG_hSth1Cas9_TH1477_AscI_finale.xdna) [525  nt] : (AflII[7] / NotI[535]) \ 
     misc_feature    5626..5646
                     /label=SV40 NLS
                     /SerialCloner_Color=&h00FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - label="SV40 NLS" - 5147..5167 \ 
     misc_feature    5153..5487
                     /label=insert from TH1477_Gblock.xdna [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from TH1477_Gblock.xdna [514  nt] : (AscI[17] / NotI[535]) \ 
     misc_feature    5488..5488
                     /label=insert from TH1477_Gblock.xdna [*] [5']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from TH1477_Gblock.xdna [514  nt] : (AscI[17] / NotI[535]) \ 
     misc_feature    5491..5498
                     /label=insert from TH1477_Gblock.xdna [*] [3'] [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from TH1477_Gblock.xdna [514  nt] : (AscI[17] / NotI[535]) \ 
     misc_feature    5499..5499
                     /label=insert from TH1477_Gblock.xdna [*] [3'] [*] [5']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from TH1477_Gblock.xdna [514  nt] : (AscI[17] / NotI[535]) \ 
     misc_feature    5503..5666
                     /label=insert from TH1477_Gblock.xdna [*] [3'] [*] [3']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from TH1477_Gblock.xdna [514  nt] : (AscI[17] / NotI[535]) \ 
     misc_feature    5626..5646
                     /label=SV40 NLS
                     /SerialCloner_Color=&h21FE06
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="PKKKRKV" \ 
     misc_feature    5653..5658
                     /label=STOPs
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="*" \ 
     misc_feature    5625..5645
                     /label=SV40 NLS
                     /SerialCloner_Color=&h00FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - label="SV40 NLS" - 5147..5167 \ 
     misc_feature    5152..5487
                     /label=insert from TH1477_Gblock.xdna [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from TH1477_Gblock.xdna [514  nt] : (AscI[17] / NotI[535]) \ 
     misc_feature    5488..5488
                     /label=insert from TH1477_Gblock.xdna [*] [5']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from TH1477_Gblock.xdna [514  nt] : (AscI[17] / NotI[535]) \ 
     misc_feature    5491..5498
                     /label=insert from TH1477_Gblock.xdna [*] [3'] [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from TH1477_Gblock.xdna [514  nt] : (AscI[17] / NotI[535]) \ 
     misc_feature    5499..5499
                     /label=insert from TH1477_Gblock.xdna [*] [3'] [*] [5']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from TH1477_Gblock.xdna [514  nt] : (AscI[17] / NotI[535]) \ 
     misc_feature    5503..5665
                     /label=insert from TH1477_Gblock.xdna [*] [3'] [*] [3']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from TH1477_Gblock.xdna [514  nt] : (AscI[17] / NotI[535]) \ 
     misc_feature    5625..5645
                     /label=SV40 NLS
                     /SerialCloner_Color=&h21FE06
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="PKKKRKV" \ 
     misc_feature    5652..5657
                     /label=STOPs
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="*" \ 
                     4349..5136
                     /label=insert from PCR(U6_sgRNA_CAG_hSth1Cas9_LMD9 (1).xdna)
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from PCR(U6_sgRNA_CAG_hSth1Cas9_LMD9 (1).xdna) [788  nt] : (SbfI[7] / AflII[800]) \ 
     misc_feature    4692..4694
                     /label=CTD Domain start
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="VDS" \ 
     misc_feature    2256..5612
                     /label=St1Cas9 LMD9:TH1477 chimera [*]
                     /SerialCloner_Color=&h0F7FFE
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5139..5141
                     /label=S963K
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="K" \ 
     misc_feature    5136..5138
                     /label=V962L
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="L" \ 
     misc_feature    5151..5153
                     /label=A967T
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="T" \ 
     misc_feature    5172..5174
                     /label=T974N
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5208..5210
                     /label=A986S
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5214..5216
                     /label=L988M
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5238..5240
                     /label=T996K
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5244..5246
                     /label=K998S
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5253..5255
                     /label=Q1001K
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5259..5261
                     /label=K1003Q
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5265..5267
                     /label=N1005E
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5268..5270
                     /label=D1006N
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5277..5279
                     /label=K1009V
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5280..5282
                     /label=K1010R
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5295..5297
                     /label=S1015E
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5298..5300
                     /label=D1016N
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5343..5345
                     /label=V1031L
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5352..5354
                     /label=T1034S
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5358..5360
                     /label=T1036N
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5361..5363
                     /label=K1037G
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5370..5372
                     /label=Q1040I
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5376..5378
                     /label=F1042L
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5385..5387
                     /label=L1045T
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5394..5396
                     /label=T1048N
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5397..5399
                     /label=P1050D
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5396..5397
                     /label=M1049 deleted
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5400..5402
                     /label=K1051T
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5403..5405
                     /label=Q1052S
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5433..5435
                     /label=D1062N
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5436..5438
                     /label=K1063R
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5454..5456
                     /label=G1069S
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5460..5462
                     /label=A1071Y
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5472..5474
                     /label=V1075S
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5481..5483
                     /label=N1077T
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5490..5492
                     /label=N1081K
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5493..5495
                     /label=S1082G
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5505..5507
                     /label=K1086I
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 gtcgacattg attattgact agctgtacaa aaaagcaggc tttaaaggaa ccaattcagt 
       61 cgactggatc cggtaccaAG GTCGGGCAGG AAGAGGGCCT ATTTCCCATG ATTCCTTCAT 
      121 ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA 
      181 CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG 
      241 TTTTAAAATT ATGTTTTAAA ATGGACTATC Atatgcttac cgtaacttga aagtatttcg 
      301 atttcttggc tttatatatc ttgtggaaag gacgaaacac ccgagacgat taatgcgtct 
      361 cgGTcTTTGT ACTCTGGTAC CAGAAGCTAC AAAGATAAGG CTTCATGCCG AAATCAACAC 
      421 CCTGTCATTT TATGGCAGGG TGTTTTTTTT Actagttatt aatagtaatc aattacgggg 
      481 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 
      541 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 
      601 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 
      661 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 
      721 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 
      781 cagtacatct acgtattagt catcgctatt accatgggtc gaggtgagcc ccacgttctg 
      841 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta 
      901 attattttgt gcagcgatgg gggcgggggg ggggggggcg cgcgccaggc ggggcggggc 
      961 ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg 
     1021 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa 
     1081 gcgcgcggcg ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct 
     1141 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg 
     1201 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt 
     1261 ggctgcgtga aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg 
     1321 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg 
     1381 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag 
     1441 cgcggccggg ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg 
     1501 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc 
     1561 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg 
     1621 tgcggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc 
     1681 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg 
     1741 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc 
     1801 gagagggcgc agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc 
     1861 gccgcacccc ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg 
     1921 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg 
     1981 ctgccgcagg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg 
     2041 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta 
     2101 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattctgc 
     2161 agtcgacggt accgcgggcc cgggatccac cggtcgccac cATGGGCGCC CCAAAGAAGA 
     2221 AGCGGAAGGT CGGTATCCAC GGAGTCCCAG CAGCCAGCGA CCTGGTGCTG GGCCTGGACA 
     2281 TCGGCATCGG CAGCGTGGGC GTGGGCATCC TGAACAAGGT GACCGGCGAG ATCATCCACA 
     2341 AGAACAGTCG CATCTTCCCT GCTGCTCAGG CTGAGAACAA CCTGGTGCGC CGCACCAACC 
     2401 GCCAGGGTCG CCGGCTTGCT CGCCGCAAGA AGCACCGGCG CGTGCGCCTG AACCGCCTGT 
     2461 TCGAGGAGAG CGGCCTGATC ACCGACTTCA CCAAGATCAG CATCAACCTG AACCCCTACC 
     2521 AGCTGCGCGT GAAGGGCCTG ACCGACGAGC TGAGCAACGA GGAGCTGTTC ATCGCCCTGA 
     2581 AGAACATGGT GAAGCACCGC GGCATCAGCT ACCTGGACGA CGCCAGCGAC GACGGCAACA 
     2641 GCAGCGTGGG CGACTACGCC CAGATCGTGA AGGAGAACAG CAAGCAGCTG GAGACCAAGA 
     2701 CCCCCGGCCA GATCCAGCTG GAGCGCTACC AGACCTACGG CCAGCTGCGC GGCGACTTCA 
     2761 CCGTGGAGAA GGACGGCAAG AAGCACCGCC TGATCAACGT GTTCCCCACC AGCGCCTACC 
     2821 GCAGCGAGGC CCTGCGCATC CTGCAGACCC AGCAGGAGTT CAACCCCCAG ATCACCGACG 
     2881 AGTTCATCAA CCGCTACCTG GAGATCCTGA CCGGCAAGCG CAAGTACTAC CACGGCCCCG 
     2941 GCAACGAGAA GAGCCGCACC GACTACGGCC GCTACCGCAC CAGCGGCGAG ACCCTGGACA 
     3001 ACATCTTCGG CATCCTGATC GGCAAGTGCA CCTTCTACCC CGACGAGTTC CGCGCCGCCA 
     3061 AGGCCAGCTA CACCGCCCAG GAGTTCAATC TGCTGAACGA CCTGAACAAC CTGACCGTGC 
     3121 CCACCGAGAC CAAGAAGCTG AGCAAGGAGC AGAAGAACCA GATCATCAAC TACGTGAAGA 
     3181 ACGAGAAGGC TATGGGCCCC GCCAAGCTGT TCAAGTACAT CGCCAAGCTG CTGAGCTGCG 
     3241 ACGTGGCCGA CATCAAGGGC TACCGCATCG ACAAGAGCGG CAAGGCCGAG ATCCACACCT 
     3301 TCGAGGCCTA CCGCAAGATG AAGACCCTGG AGACCCTGGA CATCGAGCAG ATGGACCGAG 
     3361 AGACCCTGGA CAAGCTGGCC TACGTGCTGA CCCTGAACAC CGAGCGCGAG GGCATCCAGG 
     3421 AGGCCCTGGA GCACGAGTTC GCCGACGGCA GCTTCAGCCA GAAACAGGTG GACGAGCTGG 
     3481 TGCAGTTCCG CAAGGCCAAC AGCAGCATCT TCGGCAAGGG CTGGCACAAC TTCAGCGTGA 
     3541 AGCTGATGAT GGAGCTGATC CCCGAGCTGT ACGAGACCAG CGAGGAGCAG ATGACCATCC 
     3601 TGACCCGCCT GGGCAAGCAG AAGACCACCA GCAGCAGCAA CAAGACCAAG TACATCGACG 
     3661 AGAAGCTGCT GACCGAGGAG ATCTACAACC CCGTGGTGGC CAAGAGCGTG CGCCAGGCCA 
     3721 TCAAGATCGT GAACGCCGCC ATCAAGGAGT ACGGCGACTT CGACAACATC GTGATCGAGA 
     3781 TGGCCCGCGA GACCAACGAG GACGACGAGA AGAAGGCCAT CCAGAAGATC CAGAAGGCCA 
     3841 ACAAGGACGA GAAGGACGCC GCCATGCTGA AGGCCGCCAA CCAGTACAAC GGCAAGGCCG 
     3901 AGCTGCCCCA CAGCGTGTTC CACGGCCACA AGCAGCTGGC CACCAAGATC CGCCTGTGGC 
     3961 ACCAGCAGGG CGAGCGCTGC CTGTACACCG GCAAGACCAT CAGCATCCAC GACCTGATCA 
     4021 ACAACAGCAA CCAGTTCGAG GTGGACCACA TCCTGCCCCT GAGCATCACC TTCGACGACA 
     4081 GCCTGGCCAA CAAGGTGCTG GTGTACGCCA CCGCCAACCA GGAGAAGGGC CAGCGCACCC 
     4141 CCTACCAGGC CCTGGACAGC ATGGACGACG CCTGGAGCTT CCGCGAGCTG AAGGCCTTCG 
     4201 TGCGCGAGAG CAAGACCCTG AGCAACAAGA AGAAGGAGTA TCTGCTGACC GAGGAGGACA 
     4261 TCAGCAAGTT CGACGTGCGC AAGAAGTTCA TCGAGCGCAA CCTGGTGGAC ACCCGCTACG 
     4321 CCAGCCGCGT GGTGCTGAAC GCCCtgcaGG AGCACTTCCG CGCCCACAAG ATCGACACCA 
     4381 AGGTGAGCGT GGTGCGCGGC CAGTTCACCA GCCAGCTGCG CCGCCACTGG GGCATCGAGA 
     4441 AGACCCGCGA CACCTACCAC CACCACGCCG TGGACGCCCT GATCATTGCG GCTTCTAGCC 
     4501 AGCTGAACCT GTGGAAGAAG CAGAAGAACA CCCTGGTGAG CTACAGCGAG GACCAGCTGC 
     4561 TGGACATCGA GACCGGCGAG CTGATCAGCG ACGACGAGTA CAAGGAGAGC GTGTTCAAGG 
     4621 CCCCCTACCA GCACTTCGTG GACACCCTGA AGAGCAAGGA GTTCGAGGAC AGCATCCTGT 
     4681 TCAGCTACCA GGTGGACAGC AAGTTCAACC GCAAGATCAG CGACGCCACC ATCTACGCCA 
     4741 CCCGCCAGGC CAAGGTGGGC AAGGACAAGG CCGACGAGAC CTACGTGCTG GGCAAGATCA 
     4801 AGGACATCTA CACCCAGGAC GGCTACGACG CCTTCATGAA GATCTACAAG AAGGACAAGA 
     4861 GCAAGTTCCT GATGTACCGC CACGACCCCC AGACCTTCGA GAAGGTGATC GAGCCCATCC 
     4921 TGGAGAACTA CCCCAACAAG CAGATCAACG AGAAAGGCAA GGAGGTGCCC TGCAACCCCT 
     4981 TCCTGAAGTA CAAGGAGGAG CACGGCTACA TCCGCAAGTA CAGCAAGAAG GGCAACGGCC 
     5041 CCGAGATCAA GAGCCTGAAG TACTACGACA GCAAGCTGGG CAACCACATC GACATCACCC 
     5101 CCAAGGACAG CAACAACAAG GTGGTGCTGC AGAGCcttaa gCCCTGGCGC ACCGACGTGT 
     5161 ACTTCAACAA GAACACCGGC AAGTACGAGA TCCTGGGGCT GAAGTACAGC GATATGCAGT 
     5221 TTGAGAAAGG CACAGGCAAG TACAGCATCA GCAAGGAGCA GTACGAGAAC ATCAAGGTGC 
     5281 GCGAGGGCGT GGACGAGAAC AGCGAGTTCA AGTTCACCCT GTACAAGAAC GACCTTCTGC 
     5341 TGCTGAAGGA CAGCGAGAAC GGCGAGCAAA TCCTGCTGCG CTTCACCAGC CGCAACGACA 
     5401 CCAGCAAGCA CTACGTGGAG CTGAAGCCCT ACAACCGCCA GAAGTTCGAG GGCAGCGAGT 
     5461 ACCTGATCAA GAGCCTGGGC ACCGTGGCCA AGGGcGGCCA GTGCATCAAG GGCCTGGGCA 
     5521 AGAGCAACAT CAGCATCTAC AAGGTGCGCA CCGACGTGCT GGGCAACCAG CACATCATCA 
     5581 AGAACGAGGG CGACAAGCCC AAGTTGGACT TCAGCAGGGC TGACcccaag aagaagagga 
     5641 aggtgGGATC CTGATAAGAA TTCGCggccg cactcctcag gtgcaggctg cctatcagaa 
     5701 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc 
     5761 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag 
     5821 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac 
     5881 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac 
     5941 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat 
     6001 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat 
     6061 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca 
     6121 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct 
     6181 tctcttatga agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt 
     6241 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 
     6301 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
     6361 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc 
     6421 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     6481 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 
     6541 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 
     6601 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
     6661 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
     6721 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 
     6781 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     6841 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 
     6901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
     6961 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
     7021 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
     7081 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
     7141 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg 
     7201 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     7261 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     7321 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     7381 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 
     7441 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     7501 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     7561 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     7621 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
     7681 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
     7741 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
     7801 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
     7861 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
     7921 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
     7981 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
     8041 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
     8101 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
     8161 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
     8221 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
     8281 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
     8341 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
     8401 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
     8461 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
     8521 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
     8581 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 
     8641 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 
     8701 gcacatttcc ccgaaaagtg ccacctg
//