LOCUS       Exported                9875 bp ds-DNA     circular SYN 23-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9875)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Feb 23, 2019 from SnapGene 4.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9875
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        79..341
                     /label=U6 promoter
     misc_feature    343..348
                     /label=BsmBI
     misc_feature    356..361
                     /label=BsmBI
     misc_feature    363..446
                     /label=crRNA:tracrRNA chimera
     misc_feature    365
                     /label=Wobble correction + U stretch interruption
     promoter        451..2151
                     /label=CAG promoter
                     /note="created_by=""User"" - label=""CAG promoter"" - 
                     18..1718"
     promoter        451..814
                     /label=CMV Enhancer
                     /note="SerialCloner_Color=""&h03C000"" - 
                     SerialCloner_Show=""True"" - SerialCloner_Protect=""True"" 
                     - SerialCloner_Arrow=""True"" - Color=""#ff5555"" - 
                     modified_by=""User"" - X-Transferred_From=""MSP469; MSP469 
                     updated; MSP680; - MSP977; pCMV-T7-Cas9 (MLM3613); 
                     pCMV-T7-Cas9"
     primer_bind     2146..2173
                     /label=Fw a EcoRI
     primer_bind     2158..2177
                     /label=Fragment 1.FOR
     misc_feature    2165..2173
                     /label=kozak
     misc_feature    2207..2227
                     /label=SV40 NLS
     misc_feature    2228..3319
                     /label=ABE?
     misc_feature    3320..3415
                     /label=linker
     primer_bind     complement(3400..3424)
                     /label=Fragment 1.REV
     primer_bind     complement(3400..3415)
                     /label=Rev b + St1Cas9 begining
     primer_bind     3407..3431
                     /label=Fragment 2.FOR
     misc_feature    3416..3478
                     /label=St1Cas9_LMD9 D10A
     misc_feature    3437..3439
                     /label=D10A
                     /note="note=""direct strand translation"" 
                     translation=""A"""
     misc_feature    3479..6775
                     /label=St1Cas9 LMD-9
                     /note="note=""direct strand 
                     translation""translation=""SDLVLGLDIGIGSVGVGILNKVTGEIIHKNSR
                     IFPAAQAENNLVRRTNRQGRRLARRKKHRRVRLNRLFEESGLITDFTKISINLNPYQLR
                     VKGLTDELSNEELFIALKNMVKHRGISYLDDASDDGNSSVGDYAQIVKENSKQLETKTP
                     GQIQLERYQTYGQLRGDFTVEKDGKKHRLINVFPTSAYRSEALRILQTQQEFNPQITDE
                     "
     primer_bind     6303..6331
                     /label=Fw 1 BE Orth
     primer_bind     complement(6785..6808)
                     /label=Primer 1
     primer_bind     6785..6808
                     /label=Primer 2
     misc_feature    6788..6808
                     /label=SV40 NLS
                     /note="created_by=""User"" - label=""SV40 NLS"" - 
                     5147..5167"
     misc_feature    6809..6811
                     /label=STOP
     terminator      6829..7332
                     /label=RBGpA
                     /note="translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLT - 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     - 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     - 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     - MVLLEFVTAAGI"
     misc_feature    7391..7412
                     /label=M13R-20
     promoter        complement(7427..7456)
                     /label=lac promoter
                     /note="translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLT - 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     - 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     - 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     - MVLLEFVTAAGI"
     unsure          7562..7741
                     /label=SV40 promoter
                     /note="created_by=""User"" - label=""SV40 promoter"" - 
                     5921..6100"
     unsure          7597..7674
                     /label=SV40 origin
                     /note="created_by=""User"" - Color=""#aa00aa"" - 
                     modified_by=""User"" - label=""SV40 origin"" - 5956..6033"
     terminator      7755..7886
                     /label=SV40 PA terminator
                     /note="translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLT - 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     - 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     - 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     - MVLLEFVTAAGI"
     misc_feature    7942..7961
                     /label=pUC19 R
     rep_origin      complement(8110..8729)
                     /direction=LEFT
                     /label=pBR322 origin
                     /note="translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLT - 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     - 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     - 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     - MVLLEFVTAAGI"
     rep_origin      8125
                     /direction=RIGHT
                     /label=RNaseH cleavage point _ORI
                     /note="vntifkey=""33"" - label=ORI - note="" RNaseH 
                     cleavage point "" - 867..867"
     CDS             complement(8884..9744)
                     /label=ampR
                     /note="Imported_from=""Pla - sMapper"" - 
                     modified_by=""User"" - created_by=""User"" - 
                     X-Transferred_From=""BPK1520; BPK2301; MSP469; MSP680; - 
                     MSP977; VVT1; pCAG-hCas9-nls-3XFLAG (SQT817); - 
                     pCAG-hCas9-nls-3XFLAG delta Nco"
     CDS             complement(8887..9744)
                     /label=bla gene- Ap(r) determinant _AP(R)
                     /note="vntifkey=""4"" - label=AP(R) - note="" bla gene- 
                     Ap(r) determinant "" - complement(1629..2486)"
     CDS             complement(8887..9744)
                     /label=AmpR
                     /note="vntifkey=""4"" - label=AmpR - 
                     complement(1629..2486)"
     misc_feature    9745..9809
                     /label=bla\promoter
                     /note="vntifkey=""21"" - label=bla\promoter - 2487..2551"
     promoter        complement(9786..9814)
                     /label=amp promoter
                     /note="translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQL
                     GARVGY - 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     - 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     - 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     - LRSALPAGWFIA"
ORIGIN
        1 gtcgacattg attattgact agctgtacaa aaaagcaggc tttaaaggaa ccaattcagt
       61 cgactggatc cggtaccaag gtcgggcagg aagagggcct atttcccatg attccttcat
      121 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
      181 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
      241 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
      301 atttcttggc tttatatatc ttgtggaaag gacgaaacac ccgagacgat taatgcgtct
      361 cggtctttgt actctggtac cagaagctac aaagataagg cttcatgccg aaatcaacac
      421 cctgtcattt tatggcaggg tgtttttttt actagttatt aatagtaatc aattacgggg
      481 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      541 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      601 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc
      661 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      721 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      781 cagtacatct acgtattagt catcgctatt accatgggtc gaggtgagcc ccacgttctg
      841 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
      901 attattttgt gcagcgatgg gggcgggggg ggggggggcg cgcgccaggc ggggcggggc
      961 ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg
     1021 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
     1081 gcgcgcggcg ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct
     1141 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     1201 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt
     1261 ggctgcgtga aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg
     1321 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg
     1381 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag
     1441 cgcggccggg ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg
     1501 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc
     1561 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     1621 tgcggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     1681 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg
     1741 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     1801 gagagggcgc agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc
     1861 gccgcacccc ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg
     1921 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg
     1981 ctgccgcagg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     2041 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     2101 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcgag
     2161 agccgccacc atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg
     2221 gaaagtctct gaagtcgagt ttagccacga gtattggatg aggcacgcac tgaccctggc
     2281 aaagcgagca tgggatgaaa gagaagtccc cgtgggcgcc gtgctggtgc acaacaatag
     2341 agtgatcgga gagggatgga acaggccaat cggccgccac gaccctaccg cacacgcaga
     2401 gatcatggca ctgaggcagg gaggcctggt catgcagaat taccgcctga tcgatgccac
     2461 cctgtatgtg acactggagc catgcgtgat gtgcgcagga gcaatgatcc acagcaggat
     2521 cggaagagtg gtgttcggag cacgggacgc caagaccggc gcagcaggct ccctgatgga
     2581 tgtgctgcac caccccggca tgaaccaccg ggtggagatc acagagggaa tcctggcaga
     2641 cgagtgcgcc gccctgctga gcgatttctt tagaatgcgg agacaggaga tcaaggccca
     2701 gaagaaggca cagagctcca ccgactctgg aggatctagc ggaggatcct ctggaagcga
     2761 gacaccaggc acaagcgagt ccgccacacc agagagctcc ggcggctcct ccggaggatc
     2821 ctctgaggtg gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag
     2881 ggcacgcgat gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat
     2941 cggcgagggc tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat
     3001 ggccctgaga cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta
     3061 cgtgacattc gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg
     3121 cgtggtgttt ggcgtgagga acgcaaaaac cggcgccgca ggctccctga tggacgtgct
     3181 gcactacccc ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg
     3241 tgccgccctg ctgtgctatt tctttcggat gcctagacag gtgttcaatg ctcagaagaa
     3301 ggcccagagc tccaccgact ccggaggatc tagcggaggc tcctctggct ctgagacacc
     3361 tggcacaagc gagagcgcaa cacctgaaag cagcgggggc agcagcgggg ggtcaagcga
     3421 cctggtgctg ggcctggcta tcggcatcgg cagcgtgggc gtgggcatcc tgaacaaggt
     3481 gaccggcgag atcatccaca agaacagtcg catcttccct gctgctcagg ctgagaacaa
     3541 cctggtgcgc cgcaccaacc gccagggtcg ccggcttgct cgccgcaaga agcaccggcg
     3601 cgtgcgcctg aaccgcctgt tcgaggagag cggcctgatc accgacttca ccaagatcag
     3661 catcaacctg aacccctacc agctgcgcgt gaagggcctg accgacgagc tgagcaacga
     3721 ggagctgttc atcgccctga agaacatggt gaagcaccgc ggcatcagct acctggacga
     3781 cgccagcgac gacggcaaca gcagcgtggg cgactacgcc cagatcgtga aggagaacag
     3841 caagcagctg gagaccaaga cccccggcca gatccagctg gagcgctacc agacctacgg
     3901 ccagctgcgc ggcgacttca ccgtggagaa ggacggcaag aagcaccgcc tgatcaacgt
     3961 gttccccacc agcgcctacc gcagcgaggc cctgcgcatc ctgcagaccc agcaggagtt
     4021 caacccccag atcaccgacg agttcatcaa ccgctacctg gagatcctga ccggcaagcg
     4081 caagtactac cacggccccg gcaacgagaa gagccgcacc gactacggcc gctaccgcac
     4141 cagcggcgag accctggaca acatcttcgg catcctgatc ggcaagtgca ccttctaccc
     4201 cgacgagttc cgcgccgcca aggccagcta caccgcccag gagttcaatc tgctgaacga
     4261 cctgaacaac ctgaccgtgc ccaccgagac caagaagctg agcaaggagc agaagaacca
     4321 gatcatcaac tacgtgaaga acgagaaggc tatgggcccc gccaagctgt tcaagtacat
     4381 cgccaagctg ctgagctgcg acgtggccga catcaagggc taccgcatcg acaagagcgg
     4441 caaggccgag atccacacct tcgaggccta ccgcaagatg aagaccctgg agaccctgga
     4501 catcgagcag atggaccgag agaccctgga caagctggcc tacgtgctga ccctgaacac
     4561 cgagcgcgag ggcatccagg aggccctgga gcacgagttc gccgacggca gcttcagcca
     4621 gaaacaggtg gacgagctgg tgcagttccg caaggccaac agcagcatct tcggcaaggg
     4681 ctggcacaac ttcagcgtga agctgatgat ggagctgatc cccgagctgt acgagaccag
     4741 cgaggagcag atgaccatcc tgacccgcct gggcaagcag aagaccacca gcagcagcaa
     4801 caagaccaag tacatcgacg agaagctgct gaccgaggag atctacaacc ccgtggtggc
     4861 caagagcgtg cgccaggcca tcaagatcgt gaacgccgcc atcaaggagt acggcgactt
     4921 cgacaacatc gtgatcgaga tggcccgcga gaccaacgag gacgacgaga agaaggccat
     4981 ccagaagatc cagaaggcca acaaggacga gaaggacgcc gccatgctga aggccgccaa
     5041 ccagtacaac ggcaaggccg agctgcccca cagcgtgttc cacggccaca agcagctggc
     5101 caccaagatc cgcctgtggc accagcaggg cgagcgctgc ctgtacaccg gcaagaccat
     5161 cagcatccac gacctgatca acaacagcaa ccagttcgag gtggaccaca tcctgcccct
     5221 gagcatcacc ttcgacgaca gcctggccaa caaggtgctg gtgtacgcca ccgccaacca
     5281 ggagaagggc cagcgcaccc cctaccaggc cctggacagc atggacgacg cctggagctt
     5341 ccgcgagctg aaggccttcg tgcgcgagag caagaccctg agcaacaaga agaaggagta
     5401 tctgctgacc gaggaggaca tcagcaagtt cgacgtgcgc aagaagttca tcgagcgcaa
     5461 cctggtggac acccgctacg ccagccgcgt ggtgctgaac gccctgcagg agcacttccg
     5521 cgcccacaag atcgacacca aggtgagcgt ggtgcgcggc cagttcacca gccagctgcg
     5581 ccgccactgg ggcatcgaga agacccgcga cacctaccac caccacgccg tggacgccct
     5641 gatcattgcg gcttctagcc agctgaacct gtggaagaag cagaagaaca ccctggtgag
     5701 ctacagcgag gaccagctgc tggacatcga gaccggcgag ctgatcagcg acgacgagta
     5761 caaggagagc gtgttcaagg ccccctacca gcacttcgtg gacaccctga agagcaagga
     5821 gttcgaggac agcatcctgt tcagctacca ggtggacagc aagttcaacc gcaagatcag
     5881 cgacgccacc atctacgcca cccgccaggc caaggtgggc aaggacaagg ccgacgagac
     5941 ctacgtgctg ggcaagatca aggacatcta cacccaggac ggctacgacg ccttcatgaa
     6001 gatctacaag aaggacaaga gcaagttcct gatgtaccgc cacgaccccc agaccttcga
     6061 gaaggtgatc gagcccatcc tggagaacta ccccaacaag cagatcaacg agaaaggcaa
     6121 ggaggtgccc tgcaacccct tcctgaagta caaggaggag cacggctaca tccgcaagta
     6181 cagcaagaag ggcaacggcc ccgagatcaa gagcctgaag tactacgaca gcaagctggg
     6241 caaccacatc gacatcaccc ccaaggacag caacaacaag gtggtgctgc agagcgtgag
     6301 cccctggcgc gccgacgtgt acttcaacaa gaccaccggc aagtacgaga tcctggggct
     6361 gaagtacgcc gatctgcagt ttgagaaagg cacaggcacc tacaagatca gccaggagaa
     6421 gtacaacgac atcaagaaga aggagggcgt ggacagcgac agcgagttca agttcaccct
     6481 gtacaagaac gaccttctgc tggtgaagga caccgagacc aaggagcaac agctgttccg
     6541 cttcctgagc cgcaccatgc ccaagcagaa gcactacgtg gagctgaagc cctacgacaa
     6601 gcagaagttc gagggcggcg aggccctgat caaggtgctg ggcaacgtgg ccaacagcgg
     6661 ccagtgcaag aagggcctgg gcaagagcaa catcagcatc tacaaggtgc gcaccgacgt
     6721 gctgggcaac cagcacatca tcaagaacga gggcgacaag cccaagttgg acttcagcag
     6781 ggctgacccc aagaagaaga ggaaggtgtg agcggccgca ctcctcaggt gcaggctgcc
     6841 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     6901 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     6961 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     7021 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     7081 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     7141 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     7201 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     7261 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     7321 gtccctcttc tcttatgaag atccctcgac ctgcagccca agcttggcgt aatcatggtc
     7381 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7441 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7501 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     7561 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7621 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7681 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7741 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7801 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7861 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     7921 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7981 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8041 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8101 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8161 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8221 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8281 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     8341 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8401 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8461 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8521 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     8581 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8641 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8701 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8761 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8821 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8881 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8941 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9001 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9061 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9121 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9181 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9241 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9301 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9361 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9421 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9481 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9541 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9601 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9661 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9721 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9781 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9841 ggttccgcgc acatttcccc gaaaagtgcc acctg
//
