LOCUS       pVAX_CBI_AcrIIA2_v2.xdna    3889 bp      DNA     circular   2019-11-20
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pVAX_CBI_AcrIIA2_v2 [3408  nt] : (XbaI[1933] /
             EcoRI[1205]) to Gblock_AcrIIA2_v2 [473  nt] : (EcoRI[1] / X
            baI[478])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     polyA_signal    1719..1943
                     /label=BGH\pA
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="25" - label=BGHpA - 1970..2194 \ 
     misc_feature    2116..2910
                     /label=KanR
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="21" - label=KanR - 2367..3161 \ 
     rep_origin      3210..3883
                     /label=pUC\ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="33" - label=pUCori - 3461..4134 \ 
     promoter        29..607
                     /label=CMV\promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="29" - label=CMVpromoter - 29..607 \ 
     intron          755..1133
                     /label=CB\intron
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="15" - label=CBintron - 755..1133 \ 
                     1210..1682
                     /label=insert from Gblock_AcrIIA2_v2
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from Gblock_AcrIIA2_v2 [473  nt] : (EcoRI[1] / XbaI[478]) \ 
     misc_feature    1220..1267
                     /label=NPM1 NLS
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="KRPAATKKAGQAKKKK" \ 
     misc_feature    1301..1669
                     /label=AcrIIA2
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="MTLTRAQKKYAEAMHEFINMVDDFEESTPDFAKEVLHDSDYVVITKNEKYAVALCSLSTDECEYDTNLYLDEKLVDYSTVDVNGVTYYINIVETNDIDDLEIATDEDEMKSGNQEIILKSELK" \ 
ORIGIN
        1 gactcttcgc gatgtacggg ccagatatac gcgtggagct agttattaat agtaatcaat 
       61 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 
      121 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 
      181 tcccatagta acgtcaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 
      241 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 
      301 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 
      361 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca 
      421 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat 
      481 tgacgtcaat gggagtttgt tttgcaccaa aatcaacggg actttccaaa atgtcgtaac 
      541 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 
      601 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc 
      661 catagaagac accgggaccg atccagcctc cgcggattcg aatcccggcc gggaacggtg 
      721 cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag 
      781 gcccacaaaa aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact 
      841 ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca 
      901 ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa 
      961 atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac 
     1021 aatccagcta ccattctgct tttattttat ggttgggata aggctggatt attctgagtc 
     1081 caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg 
     1141 gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agaattggga ttcgaacatc 
     1201 gattgaattc ctgtacaagA AAAGGCCGGC GGCCACGAAA AAGGCCGGCC AGGCAAAAAA 
     1261 GAAAAAGggc ggaggctctg gcggcggaag cgcggccgcc ATGacgctga cccgcgctca 
     1321 gaaaaaatac gccgaggcga tgcatgagtt tatcaatatg gttgatgact ttgaagaatc 
     1381 aacgcctgac tttgcaaaag aggttctgca cgactccgac tatgtggtca ttacaaaaaa 
     1441 cgagaaatat gccgtggcac tctgtagtct ctccacagat gaatgtgagt acgatactaa 
     1501 cttgtatttg gatgaaaagc tcgtcgatta cagcacagtt gatgtcaacg gagtgacata 
     1561 ttacatcaat atagtggaaa caaatgacat agatgatctt gaaattgcga ccgacgagga 
     1621 cgagatgaag tctggaaacc aagagattat tcttaagtcc gaactgaagt gataactcga 
     1681 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc 
     1741 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 
     1801 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 
     1861 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 
     1921 tggggatgcg gtgggctcta tggcttctac tgggcggttt tatggacagc aagcgaaccg 
     1981 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg 
     2041 gctttctcgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca agagacagga 
     2101 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 
     2161 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 
     2221 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 
     2281 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 
     2341 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 
     2401 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 
     2461 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 
     2521 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 
     2581 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 
     2641 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 
     2701 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 
     2761 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 
     2821 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 
     2881 ttctatcgcc ttcttgacga gttcttctga attattaacg cttacaattt cctgatgcgg 
     2941 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacaggtggc acttttcggg 
     3001 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 
     3061 tcatgagaca ataaccctga taaatgcttc aataatagca cgtgctaaaa cttcattttt 
     3121 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 
     3181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 
     3241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 
     3301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 
     3361 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 
     3421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 
     3481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 
     3541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 
     3601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 
     3661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 
     3721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 
     3781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 
     3841 cctttttacg gttcctgggc ttttgctggc cttttgctca catgttctt
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