LOCUS       pVAX_CBI_AcrIIA4_v2.xdna    3781 bp      DNA     circular   2019-11-20
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pVAX-CBI [3408  nt] : (XbaI[1933] / EcoRI[1205
            ]) to Gblock_AcrIIA4_v2 [365  nt] : (EcoRI[1] / XbaI[370])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     polyA_signal    1611..1835
                     /label=BGH\pA
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="25" - label=BGHpA - 1970..2194 \ 
     misc_feature    2008..2802
                     /label=KanR
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="21" - label=KanR - 2367..3161 \ 
     rep_origin      3102..3775
                     /label=pUC\ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="33" - label=pUCori - 3461..4134 \ 
     promoter        29..607
                     /label=CMV\promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="29" - label=CMVpromoter - 29..607 \ 
     intron          755..1133
                     /label=CB\intron
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="15" - label=CBintron - 755..1133 \ 
                     1210..1574
                     /label=insert from Gblock_AcrIIA4_v2
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from Gblock_AcrIIA4_v2 [365  nt] : (EcoRI[1] / XbaI[370]) \ 
     misc_feature    1220..1267
                     /label=NPM1 NLS
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="KRPAATKKAGQAKKKK" \ 
     misc_feature    1301..1561
                     /label=AcrIIA4
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="MNINDLIREIKNKDYTVKLSGTDSNSITQLIIRVNNDGNEYVISESENESIVEKFISAFKNGWNQEYEDEEEFYNDMQTITLKSELN" \ 
ORIGIN
        1 gactcttcgc gatgtacggg ccagatatac gcgtggagct agttattaat agtaatcaat 
       61 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 
      121 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 
      181 tcccatagta acgtcaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 
      241 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 
      301 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 
      361 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca 
      421 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat 
      481 tgacgtcaat gggagtttgt tttgcaccaa aatcaacggg actttccaaa atgtcgtaac 
      541 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 
      601 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc 
      661 catagaagac accgggaccg atccagcctc cgcggattcg aatcccggcc gggaacggtg 
      721 cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag 
      781 gcccacaaaa aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact 
      841 ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca 
      901 ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa 
      961 atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac 
     1021 aatccagcta ccattctgct tttattttat ggttgggata aggctggatt attctgagtc 
     1081 caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg 
     1141 gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agaattggga ttcgaacatc 
     1201 gattgaattc ctgtacaagA AAAGGCCGGC GGCCACGAAA AAGGCCGGCC AGGCAAAAAA 
     1261 GAAAAAGggc ggaggctctg gcggcggaag cgcggccgcc ATGaacatta acgacctcat 
     1321 acgagagatt aagaacaaag attacaccgt caaactgtca ggaactgata gtaactcaat 
     1381 cacccagctt attatcaggg taaacaatga tgggaatgaa tatgtgatat ctgagagcga 
     1441 aaacgagtct atcgtcgaga aattcatttc cgcttttaag aacgggtgga atcaggaata 
     1501 tgaggatgaa gaagaatttt acaatgacat gcagacgatc acgttgaaaa gtgaactgaa 
     1561 ctgataactc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc 
     1621 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 
     1681 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 
     1741 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 
     1801 tagcaggcat gctggggatg cggtgggctc tatggcttct actgggcggt tttatggaca 
     1861 gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa 
     1921 gtaaactgga tggctttctc gccgccaagg atctgatggc gcaggggatc aagctctgat 
     1981 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct 
     2041 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc 
     2101 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc 
     2161 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc 
     2221 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg 
     2281 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag 
     2341 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc 
     2401 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt 
     2461 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc 
     2521 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc 
     2581 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg 
     2641 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag 
     2701 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg 
     2761 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattattaa cgcttacaat 
     2821 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacaggtg 
     2881 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 
     2941 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatag cacgtgctaa 
     3001 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 
     3061 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     3121 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     3181 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     3241 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 
     3301 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     3361 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     3421 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     3481 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
     3541 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     3601 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     3661 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     3721 ccagcaacgc ggccttttta cggttcctgg gcttttgctg gccttttgct cacatgttct 
     3781 t
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