LOCUS       pVAX_CBI_AcrIIA5_D1126_v3    3940 bp      DNA     circular   2019-11-20
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pVAX-CBI [3408  nt] : (XbaI[1933] / EcoRI[1205
            ]) to Gblock_AcrIIA5_D1126_v3.xdna [524  nt] : (EcoRI[1] / X
            baI[529])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     polyA_signal    1770..1994
                     /label=BGH\pA
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="25" - label=BGHpA - 1970..2194 \ 
     misc_feature    2167..2961
                     /label=KanR
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="21" - label=KanR - 2367..3161 \ 
     rep_origin      3261..3934
                     /label=pUC\ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="33" - label=pUCori - 3461..4134 \ 
     promoter        29..607
                     /label=CMV\promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="29" - label=CMVpromoter - 29..607 \ 
     intron          755..1133
                     /label=CB\intron
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="15" - label=CBintron - 755..1133 \ 
                     1210..1733
                     /label=insert from Gblock_AcrIIA5_D1126_v3.xdna
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from Gblock_AcrIIA5_D1126_v3.xdna [524  nt] : (EcoRI[1] / XbaI[529]) \ 
     misc_feature    1220..1267
                     /label=NPM1 NLS
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="KRPAATKKAGQAKKKK" \ 
     misc_feature    1301..1720
                     /label=AcrIIA5_D1126
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="MAYGKSRYNSYRKRNFSISDNQRREYAKKMKELEQAFENLDGWYLSSMKDSAYKDFGKYEIRLSNHSADNRYHDLENGRLIVNVKASKLNFVDIIENKLGKIIEKIDTLDLDKYRFINATKLERDIKCYYKGYKTKKDVI" \ 
ORIGIN
        1 gactcttcgc gatgtacggg ccagatatac gcgtggagct agttattaat agtaatcaat 
       61 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 
      121 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 
      181 tcccatagta acgtcaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 
      241 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 
      301 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 
      361 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca 
      421 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat 
      481 tgacgtcaat gggagtttgt tttgcaccaa aatcaacggg actttccaaa atgtcgtaac 
      541 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 
      601 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc 
      661 catagaagac accgggaccg atccagcctc cgcggattcg aatcccggcc gggaacggtg 
      721 cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag 
      781 gcccacaaaa aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact 
      841 ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca 
      901 ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa 
      961 atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac 
     1021 aatccagcta ccattctgct tttattttat ggttgggata aggctggatt attctgagtc 
     1081 caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg 
     1141 gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agaattggga ttcgaacatc 
     1201 gattgaattc ctgtacaagA AAAGGCCGGC GGCCACGAAA AAGGCCGGCC AGGCAAAAAA 
     1261 GAAAAAGggc ggaggctctg gcggcggaag cgcggccgcc ATGGCCTATG GTAAAAGTAG 
     1321 GTATAACTCC TATCGCAAAC GCaacTTCag catcTCTgac aatCAAAGGA GAGAATACGC 
     1381 AaagaaaATG aaggaaCTTG AAcagGCATT TGAGAACCTC GACGGATGGT ACTTGAGTAG 
     1441 CATGAAAGAT TCTGCGTATA AGGATTTCGG TAAGTACGAA ATCCGACTTT CAAATCACTC 
     1501 AGCCGACAAT cgcTATCATG ACCTGGAGAA CGGCCGCTTG ATCGTGAATg tgAAAGCAAG 
     1561 CAAACTTAAC TTTGTCGATA TTATCGAGAA TAAACTCggc AAGATCATCG AAAAAATTGA 
     1621 TaccCTGGAC CTTGATAAAT ATCGCTTCAT CAATGCGACC aagCTGGAGa ggGACATTAA 
     1681 GTGCTACTAT AAAGGGtatA AGACTAAGAA AgacGTTATC tgataactcg agtctagagg 
     1741 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 
     1801 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 
     1861 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 
     1921 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 
     1981 ggtgggctct atggcttcta ctgggcggtt ttatggacag caagcgaacc ggaattgcca 
     2041 gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttctcg 
     2101 ccgccaagga tctgatggcg caggggatca agctctgatc aagagacagg atgaggatcg 
     2161 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg 
     2221 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg 
     2281 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat 
     2341 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca 
     2401 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg 
     2461 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat 
     2521 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa 
     2581 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg 
     2641 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg 
     2701 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg 
     2761 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat 
     2821 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac 
     2881 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc 
     2941 cttcttgacg agttcttctg aattattaac gcttacaatt tcctgatgcg gtattttctc 
     3001 cttacgcatc tgtgcggtat ttcacaccgc atacaggtgg cacttttcgg ggaaatgtgc 
     3061 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 
     3121 aataaccctg ataaatgctt caataatagc acgtgctaaa acttcatttt taatttaaaa 
     3181 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 
     3241 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 
     3301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 
     3361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 
     3421 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 
     3481 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 
     3541 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 
     3601 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 
     3661 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 
     3721 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 
     3781 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 
     3841 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 
     3901 ggttcctggg cttttgctgg ccttttgctc acatgttctt 
//