LOCUS       pVAX_CBI_AcrIIA6_DT1_v3.xdna    4069 bp      DNA     circular   2019-11-20
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pVAX-CBI [3408  nt] : (XbaI[1933] / EcoRI[1205
            ]) to Gblock_AcrIIA6_DT1_v3 [653  nt] : (EcoRI[1] / XbaI[658
            ])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     polyA_signal    1899..2123
                     /label=BGH\pA
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="25" - label=BGHpA - 1970..2194 \ 
     misc_feature    2296..3090
                     /label=KanR
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="21" - label=KanR - 2367..3161 \ 
     rep_origin      3390..4063
                     /label=pUC\ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="33" - label=pUCori - 3461..4134 \ 
     promoter        29..607
                     /label=CMV\promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="29" - label=CMVpromoter - 29..607 \ 
     intron          755..1133
                     /label=CB\intron
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="15" - label=CBintron - 755..1133 \ 
                     1210..1862
                     /label=insert from Gblock_AcrIIA6_DT1_v3
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from Gblock_AcrIIA6_DT1_v3 [653  nt] : (EcoRI[1] / XbaI[658]) \ 
     misc_feature    1220..1267
                     /label=NPM1 NLS
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="KRPAATKKAGQAKKKK" \ 
     misc_feature    1301..1849
                     /label=AcrIIA6_DT1
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 gactcttcgc gatgtacggg ccagatatac gcgtggagct agttattaat agtaatcaat 
       61 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 
      121 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 
      181 tcccatagta acgtcaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 
      241 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 
      301 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 
      361 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca 
      421 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat 
      481 tgacgtcaat gggagtttgt tttgcaccaa aatcaacggg actttccaaa atgtcgtaac 
      541 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 
      601 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc 
      661 catagaagac accgggaccg atccagcctc cgcggattcg aatcccggcc gggaacggtg 
      721 cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag 
      781 gcccacaaaa aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact 
      841 ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca 
      901 ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa 
      961 atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac 
     1021 aatccagcta ccattctgct tttattttat ggttgggata aggctggatt attctgagtc 
     1081 caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg 
     1141 gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agaattggga ttcgaacatc 
     1201 gattgaattc ctgtacaagA AAAGGCCGGC GGCCACGAAA AAGGCCGGCC AGGCAAAAAA 
     1261 GAAAAAGggc ggaggctctg gcggcggaag cgcggccgcc ATGAAGATCA ACaacGACAT 
     1321 AAAGGAATTG ATCCTCGAAT ACgtgTCCCG ATATTTTAAA TTCGAGAATG ACTTCTATAA 
     1381 ACTGCCGGGA ATCAAATTTA CCGATGCTAA CTGGCAGAAG TTCAAAAATG GGgaaACTtc 
     1441 cATCGAGAAG ATGGGGGCGG CCAGGGTGAA TGCGATGCTT TCATGTCTCT TCGAAGACTT 
     1501 CGAGTTGGCA ATGATTGGAA AAGCCCAAAC TAACTATTAT ATTGACAATT CTCTCAAGCT 
     1561 TAATATGCCA TTTTACGCTT ATTACGACAT GTTTAAAAAG CAGTTGCTTA TTAACTGGCT 
     1621 GAAAAATAAT CGCGACGACG TTATTggcGG CACGGGTAGG ATGTATACTG CGTCAGGGAA 
     1681 CTATATTGCA AACGCGTACC TGGAGGTGGC TCTGGAGTCT AGCtcaCTGG GCtcaGGAag 
     1741 cTATATGata CAGATGCGCT TCAAAgatTA TAGTaaagga CAAGAGCCTA TCCCATCAGG 
     1801 ACGAaagAAC CGACTTGAAT GGATCGAAAA CAATCTTGAG AACATCCGGt gataactcga 
     1861 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc 
     1921 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 
     1981 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 
     2041 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 
     2101 tggggatgcg gtgggctcta tggcttctac tgggcggttt tatggacagc aagcgaaccg 
     2161 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg 
     2221 gctttctcgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca agagacagga 
     2281 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 
     2341 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 
     2401 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 
     2461 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 
     2521 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 
     2581 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 
     2641 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 
     2701 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 
     2761 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 
     2821 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 
     2881 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 
     2941 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 
     3001 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 
     3061 ttctatcgcc ttcttgacga gttcttctga attattaacg cttacaattt cctgatgcgg 
     3121 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacaggtggc acttttcggg 
     3181 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 
     3241 tcatgagaca ataaccctga taaatgcttc aataatagca cgtgctaaaa cttcattttt 
     3301 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 
     3361 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 
     3421 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 
     3481 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 
     3541 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 
     3601 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 
     3661 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 
     3721 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 
     3781 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 
     3841 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 
     3901 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 
     3961 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 
     4021 cctttttacg gttcctgggc ttttgctggc cttttgctca catgttctt
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