LOCUS pCk3 6973 bp ds-DNA circular 18-NOV-2019 DEFINITION . KEYWORDS "Addgene" "OP.kit.position:Plate01-A03" "Type.plasmid:AP" "Description:pCk3 with lacZ blue-white screening" "Use:Acceptor plasmid for odd level position 3" "Plasmid size, bp:6973" "Bacteria resistance:Kan" "Plant resistance" "Phytozome.ID.v3" "Reference:Sauret-Gueto S, Frangedakis E, et al (in preparation)" "Primary publication:this study and https\://doi.org/10.1111/nph.15625" "Registry.ID.JHLab:HM_AP_o018" "Name.JHLab" FEATURES Location/Qualifiers misc_feature 4618..4812 /label="pVS1 oriV origin of replication" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature complement(920..940) /label="lac\operator" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 468..474 /label="SapI recog site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 2421..3050 /label="pVS1 StaA stability protein" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 480..483 /label="Loop A fusion site GGAG" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 1068..1073 /label="BsaI recog site" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 3479..4552 /label="pVS1 RepA replication protein" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 1080..1082 /label="CAG fusion site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 5482..6070 /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 1075..1078 /label="Loop F fusion site CGCT" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature complement(1084..1090) /label="SapI recog site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 2182..3182 /label="STA\region\from\pVS1\plasmid" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd CDS complement(581..902) /label="LacZ" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 433..458 /label="LB" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature complement(947..976) /label="lac\promoter" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 5156..5296 /label="basis of mobility region from pBR322" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature complement(6158..6973) /label="nptI / KanR" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 1096..1120 /label="RB" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature complement(485..490) /label="BsaI recog site" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 476..478 /label="Loop g fusion site TAC" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc ORIGIN 1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta 61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga 121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga 181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta 241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag 301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg 361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg 421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcatacag 481 gagagagacc cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 541 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg 601 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 661 gcatcaggcg ctcatccgcc attcaggctg cgcaactgtt gggaagggcg atcggcgcag 721 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg 781 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc 841 ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggcgt aatcatggtc 901 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 961 aagcatatag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 1021 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctggggt ctctcgctac 1081 agtgaagagc aattctgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta 1141 ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg 1201 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg 1261 ctatagtgca gtcggcttct gacgttcagt gcagccgtca tctgaaaacg acatgtcgca 1321 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc 1381 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa 1441 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt 1501 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat 1561 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg 1621 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga 1681 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc 1741 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt 1801 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg 1861 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct 1921 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc 1981 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg 2041 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga 2101 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt 2161 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac 2221 ctctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc 2281 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta 2341 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa 2401 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag 2461 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc 2521 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc 2581 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg 2641 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg 2701 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg 2761 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac 2821 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg 2881 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct 2941 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg 3001 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg 3061 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag 3121 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt 3181 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac 3241 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat 3301 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag 3361 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg 3421 gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt 3481 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt 3541 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc 3601 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc 3661 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt 3721 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt 3781 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg 3841 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt 3901 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga 3961 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc 4021 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca 4081 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg 4141 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat 4201 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt 4261 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg 4321 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga 4381 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg 4441 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct 4501 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga 4561 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt 4621 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg 4681 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa 4741 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct 4801 ggcctgtgca taactgtctg gccagcgcac agccgaagtg ctgcaaaaag cgcctaccct 4861 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg 4921 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc 4981 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg 5041 gtgaaaacct ctgacacatg cagctcccgg tgacggtcac agcttgtctg taagcggatg 5101 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 5161 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 5221 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 5281 aaaataccgc atcaggcgct catccgcttc ctcgctcact gactcgctgc gctcggtcgt 5341 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 5401 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 5461 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 5521 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 5581 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 5641 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 5701 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 5761 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 5821 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 5881 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 5941 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 6001 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 6061 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 6121 ctcacgttaa gggattttgg tcatgcattc taggtgatta gaaaaactca tcgagcatca 6181 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt 6241 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc 6301 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa 6361 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa 6421 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat 6481 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgagt cgaaatacgc 6541 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg 6601 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg 6661 ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct 6721 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa 6781 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc 6841 catacaatcg gtagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc 6901 catataaatc agcatccatg ttggaattta atcgcggcct tgagcaagac gtttcccgtt 6961 gaatatggct cat //