LOCUS       Exported                9951 bp ds-DNA     circular SYN 26-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pNL(CMV)Fluc/CMV/WPRE-Delta-U3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 2, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1272..1452
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      2079..2214
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    2492..2725
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     enhancer        3224..3527
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3528..3731
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3761..3770
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3767..5419
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=firefly luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    5426..6014
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5897..5908)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6089..6322
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     rep_origin      complement(8133..8721)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8892..9752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9753..9857)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
       61 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
      121 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
      181 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
      241 cttcgctatt acgccagggg aggcagagat tgcagtaagc tgagatcgca gcactgcact
      301 ccagcctggg cgacagagta agactctgtc tcaaaaataa aataaataaa tcaatcagat
      361 attccaatct tttcctttat ttatttattt attttctatt ttggaaacac agtccttcct
      421 tattccagaa ttacacatat attctatttt tctttatatg ctccagtttt ttttagacct
      481 tcacctgaaa tgtgtgtatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
      541 agcagctaac aatgctgctt gtgcctggct agaagcacaa gaggaggaag aggtgggttt
      601 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
      661 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaaa gaagacaaga
      721 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
      781 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
      841 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
      901 gagcctgcat ggaatggatg accctgagag agaagtgtta gagtggaggt ttgacagccg
      961 cctagcattt catcacgtgg cccgagagct gcatccggag tatctagatg gagttccgcg
     1021 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     1081 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     1141 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc
     1201 ccgttgacgc aaatgggcgg taggcgtgta ctctagatgc tacatataag cagctgcttt
     1261 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     1321 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgctcaaa gtagtgtgtg
     1381 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     1441 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagtaaagc cagaggagat
     1501 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     1561 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     1621 cgtcggtatt aagcggggga gaattagata aatgggaaaa aattcggtta aggccagggg
     1681 gaaagaaaca atataaacta aaacatatag tatgggcaag cagggagcta gaacgattcg
     1741 cagttaatcc tggcctttta gagacatcag aaggctgtag acaaatactg ggacagctac
     1801 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca atagcagtcc
     1861 tctattgtgt gcatcaaagg atagatgtaa aagacaccaa ggaagcctta gataagatag
     1921 aggaagagca aaacaaaagt aagaaaaagg cacagcaagc agcagctgac acaggaaaca
     1981 acagccaggt cagccaaaat taccctatag tgcagaacct ccaggggcaa atggtacatc
     2041 aggccatatc acctagaact ttaaatatga ctccgcccat cccgccccta actccgccca
     2101 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     2161 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     2221 tttgcaaatg cataaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2281 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2341 aaattttcgg gtttattaca gggacagcag agatccacaa ttggagaagt gaattatata
     2401 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     2461 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     2521 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     2581 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     2641 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     2701 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     2761 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaata
     2821 acatgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     2881 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     2941 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     3001 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctgtag
     3061 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaatcccga
     3121 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     3181 ccattcgatt agtgaacgga tctagttaac tagatctaga gtccgttaca taacttacgg
     3241 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3301 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     3361 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3421 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3481 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3541 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3601 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3661 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     3721 taagcagagc tggtttagtg aaccgtcaga tccgctagcc gccaccatgg aagatgccaa
     3781 aaacattaag aagggcccag cgccattcta cccactcgaa gacgggaccg ccggcgagca
     3841 gctgcacaaa gccatgaagc gctacgccct ggtgcccggc accatcgcct ttaccgacgc
     3901 acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc ggctggcaga
     3961 agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag
     4021 cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg tggccccagc
     4081 taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc agcccaccgt
     4141 cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat
     4201 catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc aaagcatgta
     4261 caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg tgcccgagag
     4321 cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta ccggattgcc
     4381 caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc
     4441 catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc catttcacca
     4501 cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg tcgtgctcat
     4561 gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga ttcaatctgc
     4621 cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg acaagtacga
     4681 cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga
     4741 ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc tgacagaaac
     4801 aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag taggcaaggt
     4861 ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac tgggtgtgaa
     4921 ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc
     4981 cgaggctaca aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta
     5041 ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa
     5101 gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt
     5161 cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt
     5221 cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca
     5281 ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg
     5341 actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg
     5401 cggcaagatc gccgtgtaac tcgagaatca acctctggat tacaaaattt gtgaaagatt
     5461 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5521 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5581 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5641 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5701 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5761 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5821 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5881 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5941 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6001 ggccgcctcc ccgcctgggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6061 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca aagaagacaa
     6121 gatctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6181 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6241 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6301 tcagtgtgga aaatctctag cacccaggag gtagaggttg cagtgagcca agatcgcgcc
     6361 actgcattcc agcctgggca agaaaacaag actgtctaaa ataataataa taagttaagg
     6421 gtattaaata tatttataca tggaggtcat aaaaatatat atatttgggc tgggcgcagt
     6481 ggctcacacc tgcgcccggc cctttgggag gccgaggcag gtggatcacc tgagtttggg
     6541 agttccagac cagcctgacc aacatggaga aaccccttct ctgtgtattt ttagtagatt
     6601 ttattttatg tgtattttat tcacaggtat ttctggaaaa ctgaaactgt ttttcctcta
     6661 ctctgatacc acaagaatca tcagcacaga ggaagacttc tgtgatcaaa tgtggtggga
     6721 gagggaggtt ttcaccagca catgagcagt cagttctgcc gcagactcgg cgggtgtcct
     6781 tcggttcagt tccaacaccg cctgcctgga gagaggtcag accacagggt gagggctcag
     6841 tccccaagac ataaacaccc aagacataaa cacccaacag gtccaccccg cctgctgccc
     6901 aggcagagcc gattcaccaa gacgggaatt aggatagaga aagagtaagt cacacagagc
     6961 cggctgtgcg ggagaacgga gttctattat gactcaaatc agtctcccca agcattcggg
     7021 gatcagagtt tttaaggata acttagtgtg tagggggcca gtgagttgga gatgaaagcg
     7081 tagggagtcg aaggtgtcct tttgcgccga gtcagttcct gggtgggggc cacaagatcg
     7141 gatgagccag tttatcaatc cgggggtgcc agctgatcca tggagtgcag ggtctgcaaa
     7201 atatctcaag cactgattga tcttaggttt tacaatagtg atgttacccc aggaacaatt
     7261 tggggaaggt cagaatcttg tagcctgtag ctgcatgact cctaaaccat aatttctttt
     7321 ttgttttttt ttttttattt ttgagacagg gtctcactct gtcacctagg ctggagtgca
     7381 gtggtgcaat cacagctcac tgcagcctca acgtcgtaag ctcaagcgat cctcccacct
     7441 cagcctgcct ggtagctgag actacaagcg acgccccagt taatttttgt atttttggta
     7501 gaggcagcgt tttgccgtgt ggccctggct ggtctcgaac tcctgggctc aagtgatcca
     7561 gcctcagcct cccaaagtgc tgggacaacc ggggccagtc actgcacctg gccctaaacc
     7621 ataatttcta atcttttggc taatttgtta gtcctacaaa ggcagtctag tccccaggca
     7681 aaaagggggt ttgtttcggg aaagggctgt tactgtcttt gtttcaaact ataaactaag
     7741 ttcctcctaa acttagttcg gcctacaccc aggaatgaac aaggagagct tggaggttag
     7801 aagcacgatg gaattggtta ggtcagatct ctttcactgt ctgagttata attttgcaat
     7861 ggtggttcaa agactgcccg cttctgacac cagtcgctgc attaatgaat cggccaacgc
     7921 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7981 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8041 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8101 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8161 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8221 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8281 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8341 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8401 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8461 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8521 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8581 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8641 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8701 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8761 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8821 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8881 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8941 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9001 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9061 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9121 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9181 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9241 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9301 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9361 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9421 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9481 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9541 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9601 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9661 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9721 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9781 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9841 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     9901 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg t
//
