LOCUS       BPK880:_pCAG-Dmr        5474 bp    DNA     circular UNA 21-NOV-2019
DEFINITION  .
ACCESSION   urn.local...dg-b853lzc
VERSION     urn.local...dg-b853lzc
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     enhancer        1..380
                     /note="human cytomegalovirus immediate early enhancer"
                     /X-Transferred_From="BPK1179 CAG SEQ; BPK617 CAG SEQ;
                     BPK880 CAG SEQ; JEH127 CAG SEQ; pCAG-DmrC-NLS-3xFLAG-VPR
                     (MMW948)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     BPK1179 CAG SEQ, BPK617 CAG SEQ, BPK880 CAG SEQ, JEH127
                     CAG SEQ"
                     /Transferred_Similarity="100.00%"
                     /label="CMV enhancer"
     promoter        1..1724
                     /SerialCloner_Color="&h84A4C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /modified_by="User"
                     /X-Transferred_From="BPK1179 CAG SEQ; BPK617 CAG SEQ;
                     BPK880 CAG MAP; BPK880 CAG SEQ; JEH127 CAG MAP; JEH127 CAG
                     SEQ; pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-DmrC-NLS-3xFLAG-VPR (MMW948);
                     pCAG-DmrC-NLS-3xFLAG-p65 (BPK1169);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-3xFLAG-p65 (BPK1160)"
                     /X-Transferred_Similarity="100.00% -> 98.55%; 99.94% ->
                     98.55%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     BPK1179 CAG SEQ, BPK617 CAG SEQ, BPK880 CAG SEQ, JEH127
                     CAG SEQ"
                     /Transferred_Similarity="100.00%"
                     /label="CAG promoter"
     misc_feature    381..659
                     /modified_by="User"
                     /X-Transferred_From="BPK1179 CAG SEQ; BPK617 CAG SEQ;
                     BPK880 CAG MAP; BPK880 CAG SEQ; JEH127 CAG MAP; JEH127 CAG
                     SEQ; pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-DmrC-NLS-3xFLAG-VPR (MMW948);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-3xFLAG-p65 (BPK1160)"
                     /X-Transferred_Similarity="100.00% -> 99.10%; 100.00% ->
                     99.28%; 99.28% -> 99.10%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     BPK1179 CAG SEQ, BPK617 CAG SEQ, BPK880 CAG SEQ, JEH127
                     CAG SEQ"
                     /Transferred_Similarity="100.00%"
                     /label="chicken beta-actin promoter"
     misc_feature    660..1631
                     /SerialCloner_Color="&hC01400"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /modified_by="User"
                     /X-Transferred_From="BPK1179 CAG SEQ; BPK617 CAG SEQ;
                     BPK880 CAG MAP; BPK880 CAG SEQ; JEH127 CAG MAP; JEH127 CAG
                     SEQ; pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-DmrC-NLS-3xFLAG-VPR (MMW948);
                     pCAG-DmrC-NLS-3xFLAG-p65 (BPK1169);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-3xFLAG-p65 (BPK1160)"
                     /X-Transferred_Similarity="100.00%; 100.00% -> 97.84%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     BPK1179 CAG SEQ, BPK617 CAG SEQ, BPK880 CAG SEQ, JEH127
                     CAG SEQ"
                     /Transferred_Similarity="100.00%"
                     /label="Chicken beta-actin intron"
     primer_bind     1600..1622
                     /note="Chimeric intron in CAG promoter, forward primer"
                     /X-Transferred_From="BPK1169 from Addgene; BPK1179 CAG
                     SEQ; BPK617 CAG SEQ; BPK880 CAG MAP; BPK880 CAG SEQ;
                     JEH127 CAG MAP; JEH127 CAG SEQ; MMW948 from Addgene;
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-DmrC-NLS-3xFLAG-VPR (MMW948);
                     pCAG-DmrC-NLS-3xFLAG-p65 (BPK1169);
                     pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-3xFLAG-p65 (BPK1160);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     BPK1179 CAG SEQ, BPK617 CAG SEQ, BPK880 CAG MAP, BPK880
                     CAG SEQ, JEH127 CAG MAP, JEH127 CAG SEQ,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="pCAGGS-5"
     misc_feature    1632..1724
                     /SerialCloner_Color="&hA304C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /modified_by="User"
                     /X-Transferred_From="BPK1179 CAG SEQ; BPK617 CAG SEQ;
                     BPK880 CAG MAP; BPK880 CAG SEQ; JEH127 CAG MAP; JEH127 CAG
                     SEQ; pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-DmrC-NLS-3xFLAG-VPR (MMW948);
                     pCAG-DmrC-NLS-3xFLAG-p65 (BPK1169);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-3xFLAG-p65 (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     BPK1179 CAG SEQ, BPK617 CAG SEQ, BPK880 CAG MAP, BPK880
                     CAG SEQ, JEH127 CAG MAP, JEH127 CAG SEQ,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="Rabbit globin intron"
     primer_bind     1684..1703
                     /SerialCloner_Color="&hDC143C"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""pCAG_F_primer"" -
                     translation=""MFMPSSFSYSSWATCWLLCCLIILAKNSAVDGTAGPGSTGRHHG
                     -
                     EQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGRCHLRQADPEVHLHHRQAARALAH
                     -
                     PRDHPDLRRAVLQPLPRPHEAARLLQVRHARRLRPGAHHLLQGRRQLQDPRRGEVRGR
                     - HPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLYHGRQAEERHQGELQ \"
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
                     /X-Transferred_From="BPK1169 from Addgene; BPK1179 CAG
                     SEQ; BPK617 CAG SEQ; BPK880 CAG MAP; BPK880 CAG SEQ;
                     JEH127 CAG MAP; JEH127 CAG SEQ; MMW948 from Addgene;
                     SQT367; pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-DmrC-NLS-3xFLAG-VPR (MMW948);
                     pCAG-DmrC-NLS-3xFLAG-VPR (MMW948) Addgene;
                     pCAG-DmrC-NLS-3xFLAG-p65 (BPK1169);
                     pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-3xFLAG-p65 (BPK1160);
                     pCAG-NLS-dCas9-NLS-FLAG-DmrA(x4b) (BPK1179)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     BPK1179 CAG SEQ, BPK617 CAG SEQ, BPK880 CAG MAP, BPK880
                     CAG SEQ, JEH127 CAG MAP, JEH127 CAG SEQ,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /Sequence="GCAACGTGCTGGTTATTGTG"
                     /Hairpin_Tm="41.2"
                     /Tm="58.3"
                     /Self_Dimer_Tm="3.2"
                     /%GC="50.0"
                     /modified_by="User"
                     /label="pCAG-F"
     regulatory      1769..1778
                     /SerialCloner_Color="&hB400C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="note=""direct strand translation"" \;
                     translation=""AT"" \"
                     /modified_by="User"
                     /X-Transferred_From="pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="Kozak sequence"
     ORF             1775..2353
                     /created_by="User"
                     /Transferred_From="pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880)"
                     /Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /label="DmrC-NLS-3xFLAG-p65"
     domain          1787..2065
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain
                     of human FRAP"
                     /note="binds synthetic ligands such as AP21967"
                     /X-Transferred_Translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEV
                     LEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVF
                     RRISK"
                     /X-Transferred_From="BPK1169 DmrC-p65; BPK1169 from
                     Addgene; MMW948 from Addgene"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /Transferred_From="pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880)"
                     /Transferred_Similarity="100.00%"
                     /label="DmrC (FRB T2098L)"
     sig_peptide     2078..2098
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /X-Transferred_Translation="PKKKRKV"
                     /created_by="User"
                     /X-Transferred_From="BPK1169 DmrC-p65; BPK1169 from
                     Addgene; MMW948 from Addgene; pCAG-DmrC-NLS-3xFLAG-VP64
                     (BPK880); pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="SV40 NLS"
     motif           2105..2170
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /X-Transferred_Translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /X-Transferred_From="BPK1169 DmrC-p65; BPK1169 from
                     Addgene; MMW948 from Addgene; pCAG-DmrC-NLS-3xFLAG-VP64
                     (BPK880); pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="3xFLAG"
     domain          2201..2350
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /modified_by="User"
                     /X-Transferred_From="MMW948 from Addgene;
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /X-Transferred_Similarity="100.00%; 100.00% -> 76.00%;
                     76.00%"
                     /X-Transferred_Translation="DALDDFDLDMLGSDALDDFDLDMLGSDALD
                     DFDLDMLGSDALDDFDLDML"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="VP64"
     misc_feature    complement(2431..2453)
                     /Sequence="CAGAGGGAAAAAGATCTCAGTGG"
                     /Hairpin_Tm="None"
                     /Tm="58.7"
                     /Self_Dimer_Tm="None"
                     /%GC="47.8"
                     /created_by="primer3"
                     /label="oBK23"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(2440..2459)
                     /note="Rabbit beta-globin polyA region, reverse primer"
                     /X-Transferred_From="BPK1169 from Addgene; MMW948 from
                     Addgene; pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="Bglob-pA-R"
     polya_site      2505..2560
                     /note="rabbit beta-globin polyadenylation signal"
                     /X-Transferred_From="BPK1169 DmrC-p65; BPK1169 from
                     Addgene; MMW948 from Addgene; pCAG-DmrC-NLS-3xFLAG-VP64
                     (BPK880); pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="-beta-globin poly(A) signal"
     terminator      2528..3031
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""rb_glob_PA_terminator"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHY \"
                     /modified_by="User"
                     /X-Transferred_From="pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /X-Transferred_Similarity="99.40%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1"
                     /Transferred_Similarity="100.00%"
                     /label="rb_glob_PA_terminator"
     primer_bind     complement(2559..2578)
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
                     /X-Transferred_From="BPK1169 from Addgene; MMW948 from
                     Addgene; pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="rbglobpA-R"
     primer_bind     complement(2921..2937)
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Hairpin_Tm="None"
                     /Tm="49.1"
                     /Self_Dimer_Tm="None"
                     /%GC="47.1"
                     /modified_by="User"
                     /X-Transferred_From="M13-R (-26);
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880)"
                     /Transferred_Similarity="100.00%"
                     /label="M13 Reverse"
     protein_bind    2945..2961
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /X-Transferred_From="BPK1169 DmrC-p65; BPK1169 from
                     Addgene; MMW948 from Addgene; pCAG-DmrC-NLS-3xFLAG-VP64
                     (BPK880); pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="lac operator"
     promoter        complement(2969..2999)
                     /note="promoter for the E. coli lac operon"
                     /X-Transferred_From="BPK1169 DmrC-p65; BPK1169 from
                     Addgene; MMW948 from Addgene; pCAG-DmrC-NLS-3xFLAG-VP64
                     (BPK880); pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="lac promoter"
     protein_bind    3014..3035
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /X-Transferred_From="BPK1169 DmrC-p65; BPK1169 from
                     Addgene; MMW948 from Addgene; pCAG-DmrC-NLS-3xFLAG-VP64
                     (BPK880); pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="CAP binding site"
     promoter        3094..3289
                     /note="SV40 early promoter"
                     /X-Transferred_From="BPK1169 DmrC-p65; BPK1169 from
                     Addgene; MMW948 from Addgene; pCAG-DmrC-NLS-3xFLAG-VP64
                     (BPK880); pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="SV40 promoter"
     rep_origin      3140..3275
                     /note="SV40 origin of replication"
                     /X-Transferred_From="BPK1169 DmrC-p65; BPK1169 from
                     Addgene; MMW948 from Addgene; pCAG-DmrC-NLS-3xFLAG-VP64
                     (BPK880); pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="SV40 ori"
     primer_bind     3202..3221
                     /note="SV40 promoter/origin, forward primer"
                     /X-Transferred_From="BPK1169 from Addgene; MMW948 from
                     Addgene; pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="SV40pro-F"
     polya_signal    3295..3429
                     /note="SV40 polyadenylation signal"
                     /X-Transferred_From="BPK1169 DmrC-p65; BPK1169 from
                     Addgene; MMW948 from Addgene; pCAG-DmrC-NLS-3xFLAG-VP64
                     (BPK880); pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(3332..3351)
                     /note="SV40 polyA, reverse primer"
                     /X-Transferred_From="BPK1169 from Addgene; MMW948 from
                     Addgene; pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="SV40pA-R"
     primer_bind     3386..3405
                     /note="SV40 polyA terminator, reverse primer"
                     /X-Transferred_From="BPK1169 from Addgene; MMW948 from
                     Addgene; pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179)"
                     /Transferred_Similarity="100.00%"
                     /label="EBV-rev"
     terminator      3454..3585
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""SV40_PA_terminator"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLST \"
                     /modified_by="User"
                     /X-Transferred_From="pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="SV40_PA_terminator"
     primer_bind     complement(3497..3514)
                     /note="L4440 vector, forward primer"
                     /X-Transferred_From="BPK1169 from Addgene; MMW948 from
                     Addgene; pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="L4440"
     rep_origin      order(complement(<3987..4308),complement(3668..>3986))
                     /created_by="User"
                     /label="pBR322 Origin"
     primer_bind     complement(3748..3767)
                     /note="pBR322 origin, forward primer"
                     /X-Transferred_From="BPK1169 from Addgene; MMW948 from
                     Addgene; pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322ori-F"
     cds             complement(4479..5339)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /X-Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVK
                     VKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKEL
                     TAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQ
                     LIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTT
                     GSQATMDERNRQIAEIGASLIKHW"
                     /X-Transferred_From="BPK1169 DmrC-p65; BPK1169 from
                     Addgene; MMW948 from Addgene; pCAG-DmrC-NLS-3xFLAG-VP64
                     (BPK880); pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR"
     primer_bind     5102..5121
                     /note="Ampicillin resistance gene, reverse primer"
                     /X-Transferred_From="BPK1169 from Addgene; MMW948 from
                     Addgene; pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880);
                     pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="Amp-R"
     promoter        complement(5340..5444)
                     /gene="bla"
                     /X-Transferred_From="BPK1169 DmrC-p65; BPK1169 from
                     Addgene; MMW948 from Addgene; pCAG-DmrC-NLS-3xFLAG-VP64
                     (BPK880); pCAG-DmrC-NLS-FLAG-VP64 (BPK880);
                     pCAG-NLS-dCas9-3HA-VPR(JEH127);
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179);
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-VP64 (BPK617);
                     pCAG-NLS-dCas9-NLS-FLAG-p65(MLM) (BPK1160)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BK617_BPK617_CG8_CIRCLE_9341_l1,
                     pCAG-DmrC-NLS-3xFLAG-VP64 (BPK880),
                     pCAG-NLS-dCas9-3HA-VPR(JEH127),
                     pCAG-NLS-dCas9-NLS-3xFLAG-DmrA(x4) (BPK1179),
                     pCAG-NLS-dCas9-NLS-3xFLAG-VP64 (BPK617)"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR promoter"
ORIGIN      
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc tgcagtcgac
     1741 ggtaccgcgg gcccgggatc caccggtcgc caccatggga tccagaatcc tctggcatga
     1801 gatgtggcat gaaggcctgg aagaggcatc tcgtttgtac tttggggaaa ggaacgtgaa
     1861 aggcatgttt gaggtgctgg agcccttgca tgctatgatg gaacggggac cccagactct
     1921 gaaggaaaca tcctttaatc aggcctatgg tcgagattta atggaggccc aagagtggtg
     1981 caggaagtac atgaaatcag ggaatgtcaa ggacctcctc caagcctggg acctctatta
     2041 tcatgtgttc cgacgaatct caaagggcgg cggatccccc aagaagaaga ggaaagtctc
     2101 gagcgactac aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga
     2161 cgatgacaag gctgcaggag gcggtggaag cgggcgcgcc gacgcgctgg acgatttcga
     2221 tctcgacatg ctgggttctg atgccctcga tgactttgac ctggatatgt tgggaagcga
     2281 cgcattggat gactttgatc tggacatgct cggctccgat gctctggacg atttcgatct
     2341 cgatatgtta taaagcggcc gcactcctca ggtgcaggct gcctatcaga aggtggtggc
     2401 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     2461 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     2521 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     2581 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     2641 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     2701 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     2761 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     2821 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     2881 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2941 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3001 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     3061 tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt
     3121 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3181 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     3241 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg
     3301 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3361 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3421 gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3481 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3541 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3601 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3661 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3721 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3781 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3841 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3901 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3961 ccggtaacta tcgtcttgag tccaaccgtt cagcccgacc gctgcgcctt atccggtaac
     4021 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4081 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4141 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4201 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4261 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4321 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4381 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4441 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4501 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4561 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4621 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4681 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4741 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4801 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4861 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4921 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4981 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5041 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5101 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5161 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5221 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5281 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5341 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5401 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5461 gtgccacctg ggtc
//
